FastQCFastQC Report
Wed 29 May 2019
HGKGNBGXB_n01_dpr3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGKGNBGXB_n01_dpr3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3324398
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGG143270.43096524543691817No Hit
CCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACC109360.3289618150414No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAC24350.029.7541941
TATACCG5750.026.1708985
CTACAGT34050.025.5917644
GTAGGAC25050.025.5659163
TCCTACA27800.025.4294912
TAGGACC14200.024.89154
TACAGTG36250.024.8109365
CCTACAG29900.022.7064133
AATCACG15250.022.02958734
TGTAGGA29650.021.8362522
ATCACGG15550.020.92943635
TCACGGA15250.019.96431436
GGAATAT34400.019.93896512
TCACGTC13900.019.88979557
AATATGG44450.019.44599514
CTGTAGG32050.019.438771
AGTGGAC20000.019.4221788
ATATGGC42500.019.18544815
GAATATG37300.018.95167213
CGACTAG3550.018.73023856
Produced by FastQC (version 0.11.5)