FastQCFastQC Report
Wed 29 May 2019
HGKGNBGXB_n01_dpr2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGKGNBGXB_n01_dpr2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7702142
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAC35500.021.5927451
GTAGGAC40350.021.0752643
TAGGACC21600.020.7379844
CTATACT23500.019.8058784
TCCTACA43950.018.393462
CTACAGT50250.018.246334
TAGGACG30200.018.1929454
TGTAGGA47750.017.882442
CTACACT31300.017.777194
TATACTG30150.017.5266915
TACAGTG61350.016.8274295
TACACTG50350.016.6810025
ACGAGAC56750.016.67477670
GTATAAG23950.016.5144841
CCTACAG47550.016.4121193
CTGTAGG53600.016.1949271
GTGTATA47100.016.0518441
CGACTAG8350.015.92654456
GTCCTAG19850.015.8698971
TCTATAC22100.015.6766493
Produced by FastQC (version 0.11.5)