FastQCFastQC Report
Wed 29 May 2019
HGKGNBGXB_n01_dpr1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGKGNBGXB_n01_dpr1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11865498
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAC50050.020.6307471
TATACTG42000.019.9976255
GTAGGAC52750.019.703823
CTATACT42500.019.1859574
ACGAGAC61250.017.9065370
TACACTG81300.017.5194455
GTATAGG35750.017.03611
CTACACT64250.016.9396234
GTGTAGC63300.016.8652651
TAGGACT52650.016.7501494
TAAGACT49700.016.1952614
CCTATAC38100.015.9823543
TATACAG66550.015.9334955
TGTACTG71950.015.8563525
AAGGCGT28500.015.8401576
TATGGCG30500.015.83345216
TATACCG7700.015.4527115
TAGACTG52800.015.4432415
GGCGTGC27100.015.3664038
TAGCCCT79250.015.1906254
Produced by FastQC (version 0.11.5)