FastQCFastQC Report
Mon 10 May 2021
HGJM2BGXJ_n02_ReSeq_W17_Neg_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGJM2BGXJ_n02_ReSeq_W17_Neg_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences251399
Sequences flagged as poor quality0
Sequence length151
%GC45

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAGAACAGATCCATTAGAAAACAATTATACTCCAGTTTCTTCTGTAA36881.4669907199312646No Hit
GTCCCTACACTGAGCAGCACTATTACTGTTATTGCTCCTGCTCATCATGG23500.9347690324941627No Hit
CTACTGGATCGCTTCCATGATCAAAAGGGCACATATCTCCTCTCATACAG16660.6626915779299043No Hit
ACTGAAAGCTGGTCAGAATTCCATGAAGAGCAGCTGGACCACAACTCCTA14020.5576792270454536No Hit
GAACAGATCCATTAGAAAACAATTATACTCCAGTTTCTTCTGTAACAAAT12980.5163107251818821No Hit
GTGTAGGGACAGGATAGTGGCCAGATGAAATATTTGTTACAGAAGAAACT12920.5139240808435992No Hit
CTATTACTGTTATTGCTCCTGCTCATCATGGAAATAACACTACTGAAAGC12890.5127307586744577No Hit
GTTCAGCACCGTGCATGGCAGCACTGCCAATCCAAATCCTAACCCAGCTC10740.42720933655265136No Hit
GTCCAGGAGGAGGTGGTCCTTCAACAACAGGGGGTTGTGCTGGAAAAGGC9600.38186309412527497No Hit
CTCATACAGAAACCCTTTTCATCATAATCTCTACAGCGTTTCTTTGGCAG8780.3492456215020744No Hit
GTGCTGAACACTGCAGTGTGGGGAAGTGGAGGTCCAGAACTCCCCAGAAA8530.33930127009256206No Hit
CTAAAATACGAAAGGGATTTGGGAAAGCCAAAATATGATATGGATAGAAC8340.3317435630213326No Hit
TCTCTACAGCGTTTCTTTGGCAGCGGAGGTCTTCCATAGGAGTTGTGGTC7990.3178214710480153No Hit
GTACTGGAGGTCTAAGGTTAACAGGAGGAGGACTCAGAATTGGTGGAGGG7870.3130481823714494No Hit
GCTTTCAGTAGTGTTATTTCCATGATGAGCAGGAGCAATAACAGTAATAG7670.30509270124383947No Hit
CAATTATACTCCAGTTTCTTCTGTAACAAATATTTCATCTGGCCACTATC7400.29435280172156614No Hit
GTGGTAAGGGGCCTGGTGGTGGTACTGGAGGTCTAAGGTTAACAGGAGGA7230.2875906427630977No Hit
TTCAACAACAGGGGGTTGTGCTGGAAAAGGCAGAATGCCAGGAAGATTCA7060.28082848380462927No Hit
GTGCTGGAAAAGGCAGAATGCCAGGAAGATTCACATCTTCTACCACTACT6570.2613375550419851No Hit
GATATGGATAGAACAGATCCATTAGAAAACAATTATACTCCAGTTTCTTC6190.24622214089952627No Hit
GGTACCAACAGTTGGTCTCTAAAATACGAAAGGGATTTGGGAAAGCCAAA6030.23985775599743836No Hit
AGCTACAGCAGCTCCATTAAAGATAGTTAATGGAAGTTATGATCTAAACG5950.2366755635463944No Hit
GGTAAGTGGCATCCATTTATTGTTGAACTACTTGAAGGCCCGTGGTGGGG5790.23031117864430647No Hit
AAGTAGGAGCTGGAGCAAAGAGAGACTGCGGGACAGGGATCGAGACCGGA5720.227526760249643No Hit
GTTACAGGTGGAAGGCTAGGTGGTAAGGGGCCTGGTGGTGGTACTGGAGG5700.226731212136882No Hit
GTTCTATCCATATCATATTTTGGCTTTCCCAAATCCCTTTCGTATTTTAG5570.22156014940393556No Hit
ATCAAAATACCATCTATCCAAAATAAGCTTCATAAAATCAGGGGCCAGTA5480.21798018289651114No Hit
TATCTCCTCTCATACAGAAACCCTTTTCATCATAATCTCTACAGCGTTTC5350.21280912016356468No Hit
GAATTGGTGGAGGGGGAGGAAGTCCAGGAGGAGGTGGTCCTTCAACAACA5330.21201357205080368No Hit
AAGCAGAGGTACCAACAGTTGGTCTCTAAAATACGAAAGGGATTTGGGAA5320.2116157979944232No Hit
CTTCTAAGGTGTTTCTATTGTGCCAATCGAGAAAAGGGGAAAGATTTCAT5310.21121802393804273No Hit
AAGAGAGACTGCGGGACAGGGATCGAGACCGGAGCAGAAGCCGAAGCAGA5270.20962692771252073No Hit
GTCCTGTTACAGGTGGAAGGCTAGGTGGTAAGGGGCCTGGTGGTGGTACT5180.2060469612050963No Hit
CCATAGGAGTTGTGGTCCAGCTGCTCTTCATGGAATTCTGACCAGCTTTC5100.20286476875405235No Hit
GAGCAGCACTATTACTGTTATTGCTCCTGCTCATCATGGAAATAACACTA5050.20087589847214987No Hit
ATCCAGTAGTGGTAGAAGATGTGAATCTTCCTGGCATTCTGCCTTTTCCA4960.1972959319647254No Hit
ACAGGGGGTTGTGCTGGAAAAGGCAGAATGCCAGGAAGATTCACATCTTC4910.1953070616828229No Hit
ACTTGAAGGCCCGTGGTGGGGGATGTAAAACATCCTTGTCTGGTTGGCAC4770.18973822489349598No Hit
CTCCTATGGAAGACCTCCGCTGCCAAAGAAACGCTGTAGAGATTATGATG4770.18973822489349598No Hit
GGATTTGGGAAAGCCAAAATATGATATGGATAGAACAGATCCATTAGAAA4740.1885449027243545No Hit
TTCTACCACTACTGGATCGCTTCCATGATCAAAAGGGCACATATCTCCTC4710.18735158055521303No Hit
ATCCTGTCCCTACACTGAGCAGCACTATTACTGTTATTGCTCCTGCTCAT4690.18655603244245203No Hit
ATATGATATGGATAGAACAGATCCATTAGAAAACAATTATACTCCAGTTT4610.18337383999140808No Hit
ATTATACTCCAGTTTCTTCTGTAACAAATATTTCATCTGGCCACTATCCT4600.1829760659350276No Hit
GATCAAAAGGGCACATATCTCCTCTCATACAGAAACCCTTTTCATCATAA4530.18019164754036412No Hit
GTATGAACATGGGGCTTTTTGTACCCAAACCCTCTTGGAGTGAGCTGGGC4500.17899832537122262No Hit
GGTCTAAGGTTAACAGGAGGAGGACTCAGAATTGGTGGAGGGGGAGGAAG4460.17740722914570065No Hit
CATATCTCCTCTCATACAGAAACCCTTTTCATCATAATCTCTACAGCGTT4410.17541835886379817No Hit
GTACCAACAGTTGGTCTCTAAAATACGAAAGGGATTTGGGAAAGCCAAAA4400.1750205848074177No Hit
CTTCCATAGGAGTTGTGGTCCAGCTGCTCTTCATGGAATTCTGACCAGCT4400.1750205848074177No Hit
CTGTAGAGATTATGATGAAAAGGGTTTCTGTATGAGAGGAGATATGTGCC4280.17024729613085177No Hit
GTTATGCAGAGGCAGGCATCATGCTTTAAAAGGTCGGAGCAAGTTTAAGC4160.16547400745428584No Hit
CTCCAAGAGGGTTTGGGTACAAAAAGCCCCATGTTCATACAGCAGCGACG4120.16388291122876383No Hit
TCCTAACCCAGCTCCTCAAACCAGCGAGCTGGAAATGAAGCTCTCACACC4030.16030294472133938No Hit
AATCTCTACAGCGTTTCTTTGGCAGCGGAGGTCTTCCATAGGAGTTGTGG3930.15632520415753443No Hit
GTTATTGCTCCTGCTCATCATGGAAATAACACTACTGAAAGCTGGTCAGA3920.15592743010115395No Hit
CAGGAGGAGGACTCAGAATTGGTGGAGGGGGAGGAAGTCCAGGAGGAGGT3890.15473410793201245No Hit
GTTTGAGGGAGTGCTGGCTGGAAATGTTAATCAACATCTCCGACAAGGCA3890.15473410793201245No Hit
TCCCTACACTGAGCAGCACTATTACTGTTATTGCTCCTGCTCATCATGGA3810.1515519154809685No Hit
CTGTATGAGAGGAGATATGTGCCCTTTTGATCATGGAAGCGATCCAGTAG3790.15075636736820752No Hit
GAGCAGAAGCCGAAGCAGAACACGAAGCAGAGGTACCAACAGTTGGTCTC3770.14996081925544652No Hit
CCATTAAAGATAGTTAATGGAAGTTATGATCTAAACGTAAAATTGATTTT3760.14956304519906605No Hit
GAATTAATATGTTCCCTTGTATTTCTCTAATGTTAGCTTAAGGGCTGGAT3750.14916527114268555No Hit
ATTTTATTGTTTCCTTCTAAGGTGTTTCTATTGTGCCAATCGAGAAAAGG3740.14876749708630504No Hit
GTTAACAGGAGGAGGACTCAGAATTGGTGGAGGGGGAGGAAGTCCAGGAG3740.14876749708630504No Hit
TCTTCTACCACTACTGGATCGCTTCCATGATCAAAAGGGCACATATCTCC3660.14558530463526106No Hit
GGTTAACAGGAGGAGGACTCAGAATTGGTGGAGGGGGAGGAAGTCCAGGA3610.1435964343533586No Hit
GTGCATGGCAGCACTGCCAATCCAAATCCTAACCCAGCTCCTCAAACCAG3550.14120979001507564No Hit
CCTATGGAAGACCTCCGCTGCCAAAGAAACGCTGTAGAGATTATGATGAA3540.14081201595869514No Hit
GTCCTGGTGCTATAAAACTCGGTGAACCTTAACTTTTAATATTTTTCTTC3520.14001646784593416No Hit
CTTCTACCACTACTGGATCGCTTCCATGATCAAAAGGGCACATATCTCCT3520.14001646784593416No Hit
CTCCTGCTCATCATGGAAATAACACTACTGAAAGCTGGTCAGAATTCCAT3510.13961869378955366No Hit
CCTTTAATAAAGTGTATTTAAAACTGGAGCTTTTCCCTCTGTGCTCACTC3490.13882314567679266No Hit
GCACTATTACTGTTATTGCTCCTGCTCATCATGGAAATAACACTACTGAA3470.13802759756403168No Hit
TGTTCATACAGCAGCGACGCTCCTGGTGACGGGTTTGCAGTGTTGCTGTA3460.13762982350765118No Hit
CATAAAAGGCCTCTCAATCTTTGAGATAGGCCATTAAAATCCTCGAGCCC3440.1368342753948902No Hit
ATCATAATCTCTACAGCGTTTCTTTGGCAGCGGAGGTCTTCCATAGGAGT3440.1368342753948902No Hit
CTACTGAAAGCTGGTCAGAATTCCATGAAGAGCAGCTGGACCACAACTCC3420.1360387272821292No Hit
CCACTATCCTGTCCCTACACTGAGCAGCACTATTACTGTTATTGCTCCTG3410.1356409532257487No Hit
CTCTTATCTCTTACCTTTTTTAACACAGCAAATAATTGTATTTTGTCCAA3370.13404985700022673No Hit
GCCAAAATATGATATGGATAGAACAGATCCATTAGAAAACAATTATACTC3360.13365208294384626No Hit
GTAGGAGCTGGAGCAAAGAGAGACTGCGGGACAGGGATCGAGACCGGAGC3360.13365208294384626No Hit
GTCTTGTCCTGGTGCTATAAAACTCGGTGAACCTTAACTTTTAATATTTT3360.13365208294384626No Hit
CAGTAATAGTGCTGCTCAGTGTAGGGACAGGATAGTGGCCAGATGAAATA3320.13206098671832428No Hit
CTGCGGGACAGGGATCGAGACCGGAGCAGAAGCCGAAGCAGAACACGAAG3310.13166321266194378No Hit
CTCTTGGAGTGAGCTGGGCTGGGGGGCGGTCCCCCAGGGGAAGCATGGAG3310.13166321266194378No Hit
ACACTGAGCAGCACTATTACTGTTATTGCTCCTGCTCATCATGGAAATAA3310.13166321266194378No Hit
GTCTAAGGTTAACAGGAGGAGGACTCAGAATTGGTGGAGGGGGAGGAAGT3270.1300721164364218No Hit
CTCTCATACAGAAACCCTTTTCATCATAATCTCTACAGCGTTTCTTTGGC3260.1296743423800413No Hit
TCTCTAATGTTAGCTTAAGGGCTGGATCATTGGGTAAGAAGTATTTTAAT3240.1288787942672803No Hit
CTACAGCGTTTCTTTGGCAGCGGAGGTCTTCCATAGGAGTTGTGGTCCAG3190.12688992398537782No Hit
TTAATAAAGTGTATTTAAAACTGGAGCTTTTCCCTCTGTGCTCACTCCAG3190.12688992398537782No Hit
GTATGAGAGGAGATATGTGCCCTTTTGATCATGGAAGCGATCCAGTAGTG3160.12569660181623632No Hit
GGATTTGGGGCTCCAGGCACTTGAGGGAGAAAACCACTCCGGACTGTGAA3140.12490105370347535No Hit
GGGATCGAGACCGGAGCAGAAGCCGAAGCAGAACACGAAGCAGAGGTACC3120.12410550559071436No Hit
TCCCCACACTGCAGTGTTCAGCACCGTGCATGGCAGCACTGCCAATCCAA3110.12370773153433387No Hit
CCTGTGCACTGCTGCTGGAGAGAGAAGAAACTTTCACCTGCGGCAAAACA3100.12330995747795337No Hit
GTAGAGATTATGATGAAAAGGGTTTCTGTATGAGAGGAGATATGTGCCCT3070.1221166353088119No Hit
GTGCTCACTCCAGTTTAAAAGCAAATTATAGGCTACTTTCAAAATGTCTT3070.1221166353088119No Hit
AAATAACACTACTGAAAGCTGGTCAGAATTCCATGAAGAGCAGCTGGACC3060.12171886125243139No Hit
GAATAAATAAGGAAACAAAGCTCATGTAGAATGGCCTTTGCAGCAAGGTA2980.11853666880138744No Hit
GAGGTACCAACAGTTGGTCTCTAAAATACGAAAGGGATTTGGGAAAGCCA2950.11734334663224597No Hit
CCTCTCATACAGAAACCCTTTTCATCATAATCTCTACAGCGTTTCTTTGG2930.11654779851948496No Hit
CTCCAGTTTCTTCTGTAACAAATATTTCATCTGGCCACTATCCTGTCCCT2920.11615002446310446No Hit
GAGCAAAGAGAGACTGCGGGACAGGGATCGAGACCGGAGCAGAAGCCGAA2920.11615002446310446No Hit
CTGCTAGATAGTGGTTGTGACAAGAAGATTGGTTTTGTTGGTAGTTCACT2910.11575225040672397No Hit
AGTTTCTTCTGTAACAAATATTTCATCTGGCCACTATCCTGTCCCTACAC2910.11575225040672397No Hit
ATCTTATTCTGTACATTGCCTCTTTTCTTCTGCCTCCCCCATACACCTCT2890.11495670229396297No Hit
AGCGAAAGCAGGAGTCGAAGTAGGAGCTGGAGCAAAGAGAGACTGCGGGA2870.114161154181202No Hit
GAAATAACACTACTGAAAGCTGGTCAGAATTCCATGAAGAGCAGCTGGAC2870.114161154181202No Hit
CTCTACAGCGTTTCTTTGGCAGCGGAGGTCTTCCATAGGAGTTGTGGTCC2870.114161154181202No Hit
CACTAAGGCCACTCGGCCATAAACAAGGACACCTTCCACAGAGTGCCATG2860.1137633801248215No Hit
GTCCATAACAAATCTTACTTTGCATTTTTTTTCAGAAAGATGCCATGGCA2830.11257005795568002No Hit
TTATTAAGATGTGTTTTAGAGGCAGTTTGAGGGAGTGCTGGCTGGAAATG2820.11217228389929952No Hit
CTATTGTGCCAATCGAGAAAAGGGGAAAGATTTCATTTCATAAAGTTTTT2800.11137673578653852No Hit
GAGCTGGAGCAAAGAGAGACTGCGGGACAGGGATCGAGACCGGAGCAGAA2780.11058118767377755No Hit
ATCCAAAATAAGCTTCATAAAATCAGGGGCCAGTACAGCCTGGGATGGGA2760.10978563956101654No Hit
GAATAAGATTTTGGACCTGTAGTTGTTTTGTGTGGAGAAAGAGTGATCTG2760.10978563956101654No Hit
CCCCTGGGCTCCAAACAAAGGGAATAACAATTCACACGGGATTTGGGGCT2750.10938786550463607No Hit
CTGTTATTGCTCCTGCTCATCATGGAAATAACACTACTGAAAGCTGGTCA2750.10938786550463607No Hit
ACCAACAGTTGGTCTCTAAAATACGAAAGGGATTTGGGAAAGCCAAAATA2720.10819454333549457No Hit
TATTGCTCCTGCTCATCATGGAAATAACACTACTGAAAGCTGGTCAGAAT2690.10700122116635308No Hit
TATTTCATCTGGCCACTATCCTGTCCCTACACTGAGCAGCACTATTACTG2680.10660344710997259No Hit
GTGTTATTTCCATGATGAGCAGGAGCAATAACAGTAATAGTGCTGCTCAG2680.10660344710997259No Hit
CTTTGAGATAGGCCATTAAAATCCTCGAGCCCGTTTGGTTCACAGAAGTC2680.10660344710997259No Hit
CTATAAAACTCGGTGAACCTTAACTTTTAATATTTTTCTTCTTCTTCCTC2680.10660344710997259No Hit
GATCGAGACCGGAGCAGAAGCCGAAGCAGAACACGAAGCAGAGGTACCAA2630.10461457682807013No Hit
TCATAATTCTGTCCTTGTCTGGGTTGATGTTTTTTGCCATTAACTTACCT2620.10421680277168963No Hit
GTGGTGGTACTGGAGGTCTAAGGTTAACAGGAGGAGGACTCAGAATTGGT2610.10381902871530913No Hit
CTGTGGAAGGTGTCCTTGTTTATGGCCGAGTGGCCTTAGTGTGAGCGCCG2590.10302348060254815No Hit
GTAAGGGGCCTGGTGGTGGTACTGGAGGTCTAAGGTTAACAGGAGGAGGA2590.10302348060254815No Hit
GATTTGGGAAAGCCAAAATATGATATGGATAGAACAGATCCATTAGAAAA2580.10262570654616765No Hit
CTGCAAACCCGTCACCAGGAGCGTCGCTGCTGTATGAACATGGGGCTTTT2550.10143238437702617No Hit
GTGCTATAAAACTCGGTGAACCTTAACTTTTAATATTTTTCTTCTTCTTC2550.10143238437702617No Hit
ATTACTGTTATTGCTCCTGCTCATCATGGAAATAACACTACTGAAAGCTG2550.10143238437702617No Hit
GTCTAAAACAAAAGGGGAAAAATAATGGCAGCTTCCATCCCGAAGCAGGA2550.10143238437702617No Hit
GTTCACAGAAGTCTAAAACAAAAGGGGAAAAATAATGGCAGCTTCCATCC2550.10143238437702617No Hit
CCTTAGAAGGAAACAATAAAATACATGCTTTTTAAAAGTAGCAAAGCAGG2540.10103461032064567No Hit
TTGCAATGCAGTTTATTAAGATGTGTTTTAGAGGCAGTTTGAGGGAGTGC2540.10103461032064567No Hit
GCGGGACAGGGATCGAGACCGGAGCAGAAGCCGAAGCAGAACACGAAGCA2520.10023906220788467No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)