FastQCFastQC Report
Mon 10 May 2021
HGJM2BGXJ_n02_ReSeq_Sp20_HK1073_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGJM2BGXJ_n02_ReSeq_Sp20_HK1073_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2013614
Sequences flagged as poor quality0
Sequence length151
%GC46

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCAACACTGTGTCAAGCTTTCAGGTAGACTGCTTTCTTTGGCATGTCC171140.849914631106061No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGATTCCAACACTGTGTCAAG89290.44343156136181017No Hit
GAGCAAAAGCAGGTAGATGTTGAAAGATGAGTCTTCTAACCGAGGTCGAA77340.3840855297986605No Hit
GTTGTAAGGCTTGCATAAAGGTTATTTGCTCAAAGCTATTCTCTGTAATT52800.26221510180203356No Hit
ACCCATTACTGCCTGGTCCATTCTAATGCAAAGGGGCCCTGTCACTTTCT42370.21041768680591216No Hit
GTGTCAAGCTTTCAGGTAGACTGCTTTCTTTGGCATGTCCGCAAACGATT41290.20505419608723421No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCATTCTGCTGT40440.20083293024383023No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG39120.1942775526987794No Hit
TTCTTAACCTATGTCGCATTTCTTCAATTAACCATCTTATCTCTTCAAAC36190.1797266010268105No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGATTCCAACACTGTGTCAAGCT35590.1767468839608783No Hit
GTGTTATCATTCCATTCAAGTCCTCCGATGAGGACCCCAATTGCATTTTT33940.16855266202956473No Hit
GGCTAGTACCATTCTGTTCTCATGCCTGATTAGTGGGTTGGTGGTAGTCG33870.16820502837187268No Hit
ACTTAGAGCGTTTACAGATGAAGGAGCAATAGTGGGCGAAATCTCACCAT33260.1651756493548416No Hit
CTCATGGAATGGCTAAAGACAAGACCAATCCTGTCACCTCTGACTAAGGG33060.16418241033286418No Hit
GTACTAGCCAGCACTACGGCTAAGGCCATGGAGCAGATGGCTGGATCAAG32740.16259322789770034No Hit
CTATTGCTCCTTCATCTGTAAACGCTCTAAGTAGTATCAGAGCTTCAAGT31310.15549156889056195No Hit
GAACTAGGGTGAGTCCCAATTGTCCTCATAGCCTGCACCATTTGCCTAGC31270.15529292108616646No Hit
GCATTAACCAATCCCTTGACATTTCCTCAAGAGTCATTTCAGTTAGGTAG30900.15345542889550828No Hit
CAACATGGACAGGGCAGTTAAACTATACAAGAAGCTGAAGAGGGAAATGA30600.15196557036254218No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTTTACTTT30370.15082334548726817No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCT30280.15037638792737834No Hit
CTCTTGGTCTGGACATCAGAACTGCCACTCGTGAAGGAAAGCATATAGTG30080.14938314890540094No Hit
TTCTAATGCAAAGGGGCCCTGTCACTTTCTGCTTGGGAATGAGCATTAAC29810.14804227622573143No Hit
GTATAGGTGGGTGGGTGATGTATGCCCCACACGAAAAGAATGCTCTTTCC29570.14685038939935857No Hit
GTCCTGGAAGGGAAGGTAATGGTGAGATTTCGCCCACTATTGCTCCTTCA29080.14441695379551395No Hit
GTCCATTCTAATGCAAAGGGGCCCTGTCACTTTCTGCTTGGGAATGAGCA27760.1378615762504631No Hit
GGTTAATGCTCATTCCCAAGCAGAAAGTGACAGGGCCCCTTTGCATTAGA27670.13741461869057328No Hit
GCATATAGTGGAGCGGATTCTGGAGGAAGAATCTGACGAGGCACTTAAAA27430.1362227318642004No Hit
GTGCAGACCGTGCCAGAAAGATGAGATCTTCAATTTCAGCATTCCCAGGA27170.13493152113562978No Hit
TTACTGCCTGGTCCATTCTAATGCAAAGGGGCCCTGTCACTTTCTGCTTG27050.13433557772244334No Hit
AGAATGGACCAGGCAGTAATGGGTAAAACCATCATATTGAAAGCAAACTT26520.13170349431420322No Hit
CTATCATCCCATCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGACTTGAG26500.13160417041200548No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG26090.1295680304169518No Hit
CTCTCTATCATCCCATCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGACT25760.1279291860306891No Hit
GCACTGGAACTAGGGTGAGTCCCAATTGTCCTCATAGCCTGCACCATTTG25700.12763121432409588No Hit
GTCATATTCCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCA25420.12624067969332753No Hit
ACCTAACTGAAATGACTCTTGAGGAAATGTCAAGGGATTGGTTAATGCTC25390.1260916938400309No Hit
GAGTGGAGATCTCCCATTCTCATCACTGCTTCTCCAAGTGAATCTCTGTA25100.12465149725816367No Hit
GTGCTGGCTAGTACCATTCTGTTCTCATGCCTGATTAGTGGGTTGGTGGT25090.1246018353070648No Hit
AAGCTATTCTCTGTAATTCTTAACCTATGTCGCATTTCTTCAATTAACCA25060.12445284945376821No Hit
TACTAGGCCAAGAGCCACTTCTGTGGTCACTGTTCCCATCCGGTTGTATA25050.12440318750266934No Hit
GTCCATGTTGTTTGGGTCTCCATTCCCATTTAGGGCATTTTGGACAAATC24690.12261535726311001No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAG24010.11923834458838685No Hit
GTCCTACATCGTCGAAAGATCATCAGCTGTAAATGGAACGTGTTACCCTG23960.1189900348328925No Hit
GTATATGAGACCCATGCAACTGGCAAGCGCACCAGTTGAGTAACTGAGTG23810.11824510556640946No Hit
TTCCAGGACATACTGACGAGGATGTCAAAAATGCAATTGGGGTCCTCATC23540.11690423288673996No Hit
ATACTAGGCCAAGAGCCACTTCTGTGGTCACTGTTCCCATCCGGTTGTAT23270.11556336020707048No Hit
CTTCAATGGTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCTTA23130.1148680928916863No Hit
TTCCTTGACCGGCTTCGCCGAGATCAGAAGTCCCTAAGAGGAAGAGGCAG23120.11481843094058741No Hit
CCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACA23110.11476876898948854No Hit
AGCCAGCACTACGGCTAAGGCCATGGAGCAGATGGCTGGATCAAGTGAGC23090.11466944508729082No Hit
CCCTAAGAGGAAGAGGCAGCACTCTTGGTCTGGACATCAGAACTGCCACT23060.1145204592339942No Hit
CTCATAGCCTGCACCATTTGCCTAGCCTGACTTGCGACTTCCATGGCTTC22960.1140238397230055No Hit
CTCATAGACTCAGGCACTCCTTCCGTAGAAGGCCCTCTTTTCAAACCGTA22610.11228567143454506No Hit
CCCTAGTTCCAGTGCAGGTCTAAAAGATGATCTTATTGAAAATTTGCAGG22580.11213668558124844No Hit
CTTTTAGACCTGCACTGGAACTAGGGTGAGTCCCAATTGTCCTCATAGCC22420.11134209436366653No Hit
GCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATAT22370.11109378460817218No Hit
AAGTAGTATCAGAGCTTCAAGTCGATTAAAAATCACACTAAAGTTTGCTT22150.11000122168399702No Hit
ATCCCAATGATACTTGCTGCAACAACGAGAGGATCACTTGAATCTCTGCA22100.10975291192850269No Hit
CTCATATACAACCGGATGGGAACAGTGACCACAGAAGTGGCTCTTGGCCT22070.10960392607520608No Hit
GCATAAAGGTTATTTGCTCAAAGCTATTCTCTGTAATTCTTAACCTATGT22050.10950460217300834No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAG21650.10751812412905352No Hit
GTTGTATATGAGACCCATGCAACTGGCAAGCGCACCAGTTGAGTAACTGA21550.10702150461806482No Hit
AGCCTGCACCATTTGCCTAGCCTGACTTGCGACTTCCATGGCTTCTGCTG21250.10553164608509873No Hit
CTCATATGCAATCCTTGTTCTTCGTCCATTATCGCCTCTCCAGAAGTTCC21250.10553164608509873No Hit
AGCGAAAGCAGGGTGACAAAAACATAATGGATTCCAACACTGTGTCAAGC21190.1052336743785055No Hit
ATACAAGAAGCTGAAGAGGGAAATGACATTCCATGGAGCAAAGGAAGTTG21050.10453840706312133No Hit
GTCCCAATTGTCCTCATAGCCTGCACCATTTGCCTAGCCTGACTTGCGAC20820.10339618218784732No Hit
GTTTTACCCATTACTGCCTGGTCCATTCTAATGCAAAGGGGCCCTGTCAC20490.10175733780158462No Hit
GTATCAGAGCTTCAAGTCGATTAAAAATCACACTAAAGTTTGCTTTCAAT20480.10170767585048575No Hit
ATATACAACCGGATGGGAACAGTGACCACAGAAGTGGCTCTTGGCCTAGT20410.10136004219279365No Hit
ATCAAGTGCAATATCCCAATGATACTTGCTGCAACAACGAGAGGATCACT20230.10046612707301399No Hit
CTCTTGCTCCACTTCAAGCAATAGTTGTAAGGCTTGCATAAAGGTTATTT20140.10001916951312417No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)