FastQCFastQC Report
Mon 10 May 2021
HGJM2BGXJ_n02_ReSeq_Sp19_Neg_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGJM2BGXJ_n02_ReSeq_Sp19_Neg_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24862
Sequences flagged as poor quality0
Sequence length151
%GC56

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTATGGACTGCTTCATTGATTGCTATGGACTTTCTGCCTGTTAATCACT1140.45853109162577427No Hit
CCCTTGTACCCTGCAGAGTGACAGGAGGGAGGTAAGAGAGGCGATCAGAG1020.41026466092832437No Hit
GCTAATGGTCTGTACCAATTTATTCCACTTAGGCAGGGGCACTTCTCCCT990.39819805325396185No Hit
ATCTGAGACAGAGTGGTGACATTATGCTAGATGGAAAGCAAGGCTGTCAG950.38210924302147853No Hit
GTCTCAGATGGTGCTGAGCCTCCTCACCAGCAAGACCCGTCCCTCGCACC840.3378650148821495No Hit
ACCTACAGGATCAGCCCGCAATGGCGCTTGGGCGGCGGCTTGAACGCACG780.3137317995334245No Hit
GTCACAGCTCACAGAAATGGGCTGTGACTCCCAGCTCCCATTGGGCTTGG610.24535435604537045No Hit
GTCGTACTGGCCGCCGAGCTCGACGTTGACCGATTGCTCCGGCGGGATGC610.24535435604537045No Hit
GACTCAGACTGATGGCTCCCAGGCTGAGTCCACCTGGGCTCTGAACCAAT590.2373099509291288No Hit
GTTCCAGCCCAGCGCCCAGCTGTCACTGCATCTGATGGGCGCGACCTCGT590.2373099509291288No Hit
GTCCAGCAGTGCGCGGGCACGGGCGACGCCGGCGGCATCGAGGATCTGGG570.22926554581288713No Hit
GTGTAGAGCGTGTCGGCGTAGCGCTTGTTGGCCACATTGTCGACGTTGAG570.22926554581288713No Hit
CATTAAGGAACGTGGAAAGACATGATGCTAAATAAGAGGAGTTGTGTGAC570.22926554581288713No Hit
CACCCAGCCCTGTGCATAAAGGATCTCTGTCCTGTTCAGTTCAGCCATGC570.22926554581288713No Hit
GTCTGAGTCTGTGTGCTACAAGGCACGAAGGGTGCTGCATGAAACAGAGC560.2252433432547663No Hit
TTCCTGGGTCTTCTCTGCCTTTTCTTTGCAACAGAAAGAGAAGGGATTTA560.2252433432547663No Hit
TCCCAGTCCACTCCCACAACATAACATGACACAGAGATCACTCCTCAGCT560.2252433432547663No Hit
CTTCAAGCCCTTTCTTCTCTAAGTGGCCCCAGTAGAGGGAGGGAGTCCCT540.21719893813852467No Hit
CATCTAAGGAGCATGGCCTATTGGTTCAGAGCCCAGGTGGACTCAGCCTG530.21317673558040381No Hit
ACATAACATGACACAGAGATCACTCCTCAGCTGGCAAAGCACTGCACAGC530.21317673558040381No Hit
GTGCTGGAGATCACTTTGCCTTCCTTTCTCAGGACGTCACACTGCCTTGC530.21317673558040381No Hit
ATCCAGAGCAGTGCCGACAGCTCCCTCCCTCCCCCCTGCCGGAGGAGGAC510.20513233046416218No Hit
GAGGAGGAAGGAGCTCTCCCCTGCGTTTCTTTGAGAGTGGAACGGAGAGG500.20111012790604135No Hit
TCTCAGAGCCTTCCCTGGCTTTTCCTCCTGTCGCAGGGAGAAGTGCCCCT490.19708792534792052No Hit
GCTCTGAGATGGCCCCTTAAGGAATTGGTTGTTGCTCTCCTCACTCACGG480.1930657227897997No Hit
GTCTGTGAGAGGCAAGAGTTACCTTATTCCCACAGCCTGAACCGCCTTCT470.18904352023167886No Hit
TTGTAGCACACAGACTCAGACTGATGGCTCCCAGGCTGAGTCCACCTGGG450.1809991151154372No Hit
GTGTTGCGCTCCTGCAGCTTGGTCGCGCGGAAGATGCCGAAGCGCGTCGT450.1809991151154372No Hit
GTCCTGTTCAGTTCAGCCATGCAATGTCTGTGAGAGGCAAGAGTTACCTT450.1809991151154372No Hit
CTCCCACACTCGCTCCCTCTCTCATTCTTTCCTTCTCTTACCGCCACGCT440.1769769125573164No Hit
GCCGTAGACCTCCCAGGCGTCGGTGATGCGGCCGGTGATGTCGACGTCCA440.1769769125573164No Hit
CTGCAGAGAGGCAGACCTGGGGAACTGATGGTCCACGGAGGAGGAAGGAG440.1769769125573164No Hit
GGCCTGGACGTCGACATCACCGGCCGCATCACCGACGCCTGGGAGGTCTA430.17295470999919557No Hit
GCTATGGACTTTCTGCCTGTTAATCACTATTTCTCATCCCAGTCCACTCC420.16893250744107474No Hit
CTCTCATTCTTTCCTTCTCTTACCGCCACGCTGCAGAGAGGCAGACCTGG420.16893250744107474No Hit
CCTGTAGGTCGTCCACAGGCTGCCGGAGTAGCGCGGCGTCAGCGACGGCC410.1649103048829539No Hit
CTCCTCAGCTGGCAAAGCACTGCACAGCCATCTCCTCTTCCTCAAAACAA410.1649103048829539No Hit
GTCCACGGAGGAGGAAGGAGCTCTCCCCTGCGTTTCTTTGAGAGTGGAAC400.16088810232483308No Hit
GGATTTAGGAGGAGGGGGAACTGAAATCCCTTTCATTCGTGCCAGCAGTG400.16088810232483308No Hit
GCTTCATTGATTGCTATGGACTTTCTGCCTGTTAATCACTATTTCTCATC390.15686589976671225No Hit
CCTTAGATGTCACACAACTCCTCTTATTTAGCATCATGTCTTTCCACGTT390.15686589976671225No Hit
GTACCAATTTATTCCACTTAGGCAGGGGCACTTCTCCCTGCGACAGGAGG390.15686589976671225No Hit
CTGCTGGACACCGCACCCTGGGCCGATGGCCGCAGCAGCGCCGGCACCCA390.15686589976671225No Hit
ATTCAAAGCAGCTGTTCACCCACTAGAGTGGGCTCCCTTCAGCAACCTCC390.15686589976671225No Hit
GTGCTACAAGGCACGAAGGGTGCTGCATGAAACAGAGCTCAGCCTGACAG390.15686589976671225No Hit
CCACAGGAGAGGGGAGATGCTCTCCTGGCTCAGCAGCAAGAGGGAAGCTT380.15284369720859142No Hit
GTGCAATTGTCCTCACATTAAGGAACGTGGAAAGACATGATGCTAAATAA380.15284369720859142No Hit
GTGTAGCGGTTGAACAGCGGCGGCAGCACGCGCTTGGGCAGCGTGGCCGA370.14882149465047062No Hit
CTCCTGGCTCAGCAGCAAGAGGGAAGCTTGAGGCTGGAAGGGATGGGTGC370.14882149465047062No Hit
ATTTAAAGGGTGCAGAAGGGGTCTTGGGGGCTGCAGTGCTGGAGATCACT370.14882149465047062No Hit
GAGTGGGAGGGCGCAAGAAAGACTCCAAGCAATTTTTCTATCTCTTTCAG360.1447992920923498No Hit
TCTCTGCCTTTTCTTTGCAACAGAAAGAGAAGGGATTTAGGAGGAGGGGG360.1447992920923498No Hit
GTACTGTGGTGTTCAGCCATCTCAGAAATCTGCTGCTGAGCATGAGTCAG360.1447992920923498No Hit
GCCTGAACCGCCTTCTCTCTCCATCGTGGCTGGGAGGCTGCTCCCATTCA360.1447992920923498No Hit
CTCCTGTGGCAGTAAACTCACTGGGTGATGCCGTGAGTGAGGAGAGCAAC360.1447992920923498No Hit
CGCTTGGGCAGCGTGGCCGAGAAGAAGCCGGGATGGCCGTCCAGCGCCTG350.14077708953422896No Hit
GCCATCTCCTCTTCCTCAAAACAAACAAAAACAAAAAACAACAAAAAAAC350.14077708953422896No Hit
GTGCAGAAGGGGTCTTGGGGGCTGCAGTGCTGGAGATCACTTTGCCTTCC340.13675488697610813No Hit
CCTTAATGTGAGGACAATTGCACCAAGCCCAATGGGAGCTGGGAGTCACA340.13675488697610813No Hit
GCTCAGGGGCGACTACGACAGCTTCAACTACAGCTACAACGCGACCGGCA340.13675488697610813No Hit
GTATCAGGAGATAAGATCTTATTCTGAGCCCTTCAAGCCCTTTCTTCTCT340.13675488697610813No Hit
CTACAGGATCAGCCCGCAATGGCGCTTGGGCGGCGGCTTGAACGCACGCA340.13675488697610813No Hit
CGCCTATACCTCACGCTCGGCCGGCGCCTATCTGCAGGACATGATCTCGA340.13675488697610813No Hit
GTGTGTGTGTGTGTGTTGTTCTTTTTTTTTGTTTGTTTGTTTTGTGATTT330.1327326844179873No Hit
CTTTAAGCAGCCTCAAAGTTGGCTCTCACCACTGCTGGCACGAATGAAAG330.1327326844179873No Hit
CTCCCACTCCCTGCCCATTGCATGCACACATACACACACCCAGCCCTGTG330.1327326844179873No Hit
CCCACTAGAGTGGGCTCCCTTCAGCAACCTCCTCCTTCTCCCCTCCCTTT330.1327326844179873No Hit
GCCTGGGAGCCATCAGTCTGAGTCTGTGTGCTACAAGGCACGAAGGGTGC330.1327326844179873No Hit
GAACGGAGAGGCAGAGAGATGATGAAGGAGCGTTTTGGTTGCTGCCTGGA330.1327326844179873No Hit
TCTCTGTCCTGTTCAGTTCAGCCATGCAATGTCTGTGAGAGGCAAGAGTT330.1327326844179873No Hit
CCCTCGCACCCATCCCTTCCAGCCTCAAGCTTCCCTCTTGCTGCTGAGCC330.1327326844179873No Hit
ACATGACACAGAGATCACTCCTCAGCTGGCAAAGCACTGCACAGCCATCT330.1327326844179873No Hit
GTACGACAGCGCCGACGGCAAGCTGACGACGCGCTTCGGCATCTTCCGCG320.12871048185986647No Hit
GCCATGCAATGTCTGTGAGAGGCAAGAGTTACCTTATTCCCACAGCCTGA320.12871048185986647No Hit
CACATACACACACCCAGCCCTGTGCATAAAGGATCTCTGTCCTGTTCAGT320.12871048185986647No Hit
TCTCTAAGTGGCCCCAGTAGAGGGAGGGAGTCCCTAAGGTCCCACTGCAC320.12871048185986647No Hit
GCTTAAAGAGAAGAGATTTCCAAGTGTGTGTGTGTGTGTGTGTTGTTCTT310.12468827930174563No Hit
CACTTGGAAATCTCTTCTCTTTAAGCAGCCTCAAAGTTGGCTCTCACCAC310.12468827930174563No Hit
TCCTGCAGGCGGTCAGAACTTGTAGGTGCCGGTCAGCGCGACGATGCGGC310.12468827930174563No Hit
GTACAAGGGTGGGCTCTGGGGGGCACAGGGCCATTGAAGTCCCCAAGCAG310.12468827930174563No Hit
GGAGAGGGGAGATGCTCTCCTGGCTCAGCAGCAAGAGGGAAGCTTGAGGC300.1206660767436248No Hit
GTGCTGAGCCTCCTCACCAGCAAGACCCGTCCCTCGCACCCATCCCTTCC300.1206660767436248No Hit
GCACAGCCATCTCCTCTTCCTCAAAACAAACAAAAACAAAAAACAACAAA300.1206660767436248No Hit
GGTCTACAGCGACAGCCCCGGACTGACGTTCGCGCAACGCAGCAACTTCT300.1206660767436248No Hit
GTCCAGGCCTGCGGCGTGGCGCTTGCCGGACAGGGTCACGACATTGGTGT300.1206660767436248No Hit
ACCGTGTACTCGGCCATCAGGTTGGCGATGATGAACTTGGGCGCATAAAA300.1206660767436248No Hit
GCCTAGCTGTGAGCGTGGCATTCTGAGGCATATGTACTGTGGTGTTCAGC290.11664387418550398No Hit
CTGCACAGCCATCTCCTCTTCCTCAAAACAAACAAAAACAAAAAACAACA290.11664387418550398No Hit
AGCCTGAACCGCCTTCTCTCTCCATCGTGGCTGGGAGGCTGCTCCCATTC290.11664387418550398No Hit
CCTGCAGCTTGGTCGCGCGGAAGATGCCGAAGCGCGTCGTCAGCTTGCCG290.11664387418550398No Hit
GTATTTAAAGGGTGCAGAAGGGGTCTTGGGGGCTGCAGTGCTGGAGATCA290.11664387418550398No Hit
ATCCTGTAGGTCGTCCACAGGCTGCCGGAGTAGCGCGGCGTCAGCGACGG290.11664387418550398No Hit
CTCTTACCGCCACGCTGCAGAGAGGCAGACCTGGGGAACTGATGGTCCAC280.11262167162738315No Hit
GATTTAGGAGGAGGGGGAACTGAAATCCCTTTCATTCGTGCCAGCAGTGG280.11262167162738315No Hit
CTCCTCACTCACGGCATCACCCAGTGAGTTTACTGCCACAGGAGAGGGGA280.11262167162738315No Hit
GGCAGAGAGATGATGAAGGAGCGTTTTGGTTGCTGCCTGGATTAAAAAAG280.11262167162738315No Hit
CTTTAATCCCTGGGAACAGAGGCACGATTCAAAGCAGCTGTTCACCCACT280.11262167162738315No Hit
CTCCCTCTCTCATTCTTTCCTTCTCTTACCGCCACGCTGCAGAGAGGCAG280.11262167162738315No Hit
CACTTAGGCAGGGGCACTTCTCCCTGCGACAGGAGGAAAAGCCAGGGAAG280.11262167162738315No Hit
GCAGAGAGGCAGACCTGGGGAACTGATGGTCCACGGAGGAGGAAGGAGCT280.11262167162738315No Hit
CCTGGGAACAGAGGCACGATTCAAAGCAGCTGTTCACCCACTAGAGTGGG280.11262167162738315No Hit
ACACAGAGATCACTCCTCAGCTGGCAAAGCACTGCACAGCCATCTCCTCT280.11262167162738315No Hit
TGATGAAGGAGCGTTTTGGTTGCTGCCTGGATTAAAAAAGAATACATAAA280.11262167162738315No Hit
GAGTTACCTTATTCCCACAGCCTGAACCGCCTTCTCTCTCCATCGTGGCT270.10859946906926234No Hit
TACACACACCCAGCCCTGTGCATAAAGGATCTCTGTCCTGTTCAGTTCAG270.10859946906926234No Hit
TTCCTTCTCTTACCGCCACGCTGCAGAGAGGCAGACCTGGGGAACTGATG270.10859946906926234No Hit
CCTAAATCCCTTCTCTTTCTGTTGCAAAGAAAAGGCAGAGAAGACCCAGG270.10859946906926234No Hit
ATGCTAGATGGAAAGCAAGGCTGTCAGGCTGAGCTCTGTTTCATGCAGCA260.1045772665111415No Hit
GATGATGAAGGAGCGTTTTGGTTGCTGCCTGGATTAAAAAAGAATACATA260.1045772665111415No Hit
CTTGTACCCTGCAGAGTGACAGGAGGGAGGTAAGAGAGGCGATCAGAGTT260.1045772665111415No Hit
TGCCAGCAGTGGTGAGAGCCAACTTTGAGGCTGCTTAAAGAGAAGAGATT260.1045772665111415No Hit
GCTCAGCACCATCTGAGACAGAGTGGTGACATTATGCTAGATGGAAAGCA260.1045772665111415No Hit
CCCACAGCCTGAACCGCCTTCTCTCTCCATCGTGGCTGGGAGGCTGCTCC260.1045772665111415No Hit
GGTCTTGCTGGTGAGGAGGCTCAGCACCATCTGAGACAGAGTGGTGACAT260.1045772665111415No Hit
CTATTTCTCATCCCAGTCCACTCCCACAACATAACATGACACAGAGATCA260.1045772665111415No Hit
GCCTTGCCTGGGGAAGGCTGGCTGGGAAAAAAATGCCCATGTCTCGCCTC260.1045772665111415No Hit
AAATAAGAGGAGTTGTGTGACATCTAAGGAGCATGGCCTATTGGTTCAGA250.10055506395302068No Hit
ATGCTGGCATTGGCGGGGCTCAGCGAATAGGTGTCGCCCGAGGTGTTGAA250.10055506395302068No Hit
GCTCTACACCGGCCATTACGTTCCGGGCAAGGGCCGCATCGTCGCGCTGA250.10055506395302068No Hit
GGATGGGTGCGAGGGACGGGTCTTGCTGGTGAGGAGGCTCAGCACCATCT250.10055506395302068No Hit
GTGCATAAAGGATCTCTGTCCTGTTCAGTTCAGCCATGCAATGTCTGTGA250.10055506395302068No Hit
GAGGAGGAGGAGGCCAACGGGATGCGGGAGGAAGGAGCCAGAGCGTGGCT250.10055506395302068No Hit
GAGGCAGACCTGGGGAACTGATGGTCCACGGAGGAGGAAGGAGCTCTCCC250.10055506395302068No Hit
GCACTGCACAGCCATCTCCTCTTCCTCAAAACAAACAAAAACAAAAAACA250.10055506395302068No Hit
CTCGACGACCGTGTACTCGGCCATCAGGTTGGCGATGATGAACTTGGGCG250.10055506395302068No Hit
AGTGGAAGCTGCTGGCCGGCCTGCGCTACGACAAGCTCAGGGGCGACTAC250.10055506395302068No Hit
CTGTCGTACTGGCCGCCGAGCTCGACGTTGACCGATTGCTCCGGCGGGAT250.10055506395302068No Hit
GTTAATCACTATTTCTCATCCCAGTCCACTCCCACAACATAACATGACAC250.10055506395302068No Hit
GTGCTGCATGAAACAGAGCTCAGCCTGACAGCCTTGCTTTCCATCTAGCA250.10055506395302068No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)