FastQCFastQC Report
Mon 10 May 2021
HGJM2BGXJ_n02_ReSeq_Sm18_HK1073_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGJM2BGXJ_n02_ReSeq_Sm18_HK1073_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2764759
Sequences flagged as poor quality0
Sequence length151
%GC45

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCAACACTGTGTCAAGCTTTCAGGTAGACTGCTTTCTTTGGCATGTCC184910.6688105545546645No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGATTCCAACACTGTGTCAAG127620.46159538679501544No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAAAGAATAAAAGAACTAA97210.35160388301475826No Hit
GAGCAAAAGCAGGTAGATGTTGAAAGATGAGTCTTCTAACCGAGGTCGAA85640.30975575086291424No Hit
GTTGTAAGGCTTGCATAAAGGTTATTTGCTCAAAGCTATTCTCTGTAATT84860.3069345284706551No Hit
GCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATAT70110.2535844896426777No Hit
GTGTTATCATTCCATTCAAGTCCTCCGATGAGGACCCCAATTGCATTTTT63770.2306530153261098No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG58460.21144700134803793No Hit
CTATTGCTCCTTCATCTGTAAACGCTCTAAGTAGTATCAGAGCTTCAAGT58000.209783203526962No Hit
GCATATAGTGGAGCGGATTCTGGAGGAAGAATCTGACGAGGCACTTAAAA57980.20971086449126305No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCATTCTGCTGT55810.20186207911792675No Hit
TTCTTAACCTATGTCGCATTTCTTCAATTAACCATCTTATCTCTTCAAAC54740.19799194070803278No Hit
AAGCTATTCTCTGTAATTCTTAACCTATGTCGCATTTCTTCAATTAACCA53870.19484519265512834No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG49980.18077525021168211No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGATTCCAACACTGTGTCAAGCT49800.18012419889039152No Hit
GTATAGGTGGGTGGGTGATGTATGCCCCACACGAAAAGAATGCTCTTTCC47700.17252860014200153No Hit
GTGTCAAGCTTTCAGGTAGACTGCTTTCTTTGGCATGTCCGCAAACGATT46980.16992439485683924No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGTTCTTA45520.1646436452508157No Hit
GGTTAATGCTCATTCCCAAGCAGAAAGTGACAGGGCCCCTTTGCATTAGA45030.1628713388761914No Hit
CTCTTGGTCTGGACATCAGAACTGCCACTCGTGAAGGAAAGCATATAGTG42020.15198431400349904No Hit
GCTTTAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATAT41330.14948861727188517No Hit
AATATATTCAATATGGAAAGAATAAAAGAACTAAGAAATTTGATGTCGCA40980.1482226841471535No Hit
GTAATGGGTAAAACCATCATATTGAAAGCAAACTTTAGTGTGATTTTTAA40880.14786098896865876No Hit
GAATAAAAGAACTAAGAAATTTGATGTCGCAATCTCGCACTCGCGAGATA39970.14456956284435643No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTTTACTTT39770.1438461724873669No Hit
ACTTAGAGCGTTTACAGATGAAGGAGCAATAGTGGGCGAAATCTCACCAT39710.14362915538027005No Hit
GTACTAGCCAGCACTACGGCTAAGGCCATGGAGCAGATGGCTGGATCAAG39700.14359298586242056No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAG39560.1430866126125279No Hit
AAGTAGTATCAGAGCTTCAAGTCGATTAAAAATCACACTAAAGTTTGCTT39360.14236322225553835No Hit
AGAATGGACCAGGCAGTAATGGGTAAAACCATCATATTGAAAGCAAACTT39140.1415674928628499No Hit
GCATAAAGGTTATTTGCTCAAAGCTATTCTCTGTAATTCTTAACCTATGT38930.1408079329880109No Hit
GTTCTTATCTCTTGCTCCACTTCAAGCAATAGTTGTAAGGCTTGCATAAA37990.13740799831016012No Hit
ACCTAACTGAAATGACTCTTGAGGAAATGTCAAGGGATTGGTTAATGCTC37600.13599738711403056No Hit
GTATAACAATAGAGAGAATGGTTCTCTCCGCATTTGATGAAAGGAGGAAC37180.13447826736435256No Hit
GGTCAAATATATTCAATATGGAAAGAATAAAAGAACTAAGAAATTTGATG36910.13350169038241672No Hit
CTCATAGACTCAGGCACTCCTTCCGTAGAAGGCCCTCTTTTCAAACCGTA36890.13342935134671774No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAACATCATGG36810.13313999520392195No Hit
GTCCTGGAAGGGAAGGTAATGGTGAGATTTCGCCCACTATTGCTCCTTCA36490.1319825706327387No Hit
GGCTAGTACCATTCTGTTCTCATGCCTGATTAGTGGGTTGGTGGTAGTCG36290.13125918027574918No Hit
ACCCATTACTGCCTGGTCCATTCTAATGCAAAGGGGCCCTGTCACTTTCT35200.12731670283015625No Hit
GCAAAAGCAGGTCAAATATATTCAATATGGAAAGAATAAAAGAACTAAGA35160.12717202475875836No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCT34630.12525504031273613No Hit
CTGTAATTCTTAACCTATGTCGCATTTCTTCAATTAACCATCTTATCTCT34150.12351890345596125No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC33680.12181893611703587No Hit
GAACTAGGGTGAGTCCCAATTGTCCTCATAGCCTGCACCATTTGCCTAGC33610.12156574949208955No Hit
CTATCATCCCATCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGACTTGAG33550.12134873238499269No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT33320.12051683347445473No Hit
CTCATGGAATGGCTAAAGACAAGACCAATCCTGTCACCTCTGACTAAGGG33030.11946791745681992No Hit
GAGTGGAGATCTCCCATTCTCATCACTGCTTCTCCAAGTGAATCTCTGTA32210.11650201699316287No Hit
CCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACA31930.11548927049337754No Hit
CAACATGGACAGGGCAGTTAAACTATACAAGAAGCTGAAGAGGGAAATGA31750.11483821917208697No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCT30440.11010001233380559No Hit
CTTGTGAACAGATTGCTGATGCCCAACATCGGTCCCACAGGCAGATGGCG30130.10897875728047182No Hit
GTCATATTCCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCA30130.10897875728047182No Hit
AGCCAGCACTACGGCTAAGGCCATGGAGCAGATGGCTGGATCAAGTGAGC29490.10666390813810535No Hit
GGGTAAAACCATCATATTGAAAGCAAACTTTAGTGTGATTTTTAATCGAC29430.10644689103100849No Hit
CTCCCATTCTCATCACTGCTTCTCCAAGTGAATCTCTGTAGAGTTTCAGA29240.10575967019186845No Hit
CTCTTGCTCCACTTCAAGCAATAGTTGTAAGGCTTGCATAAAGGTTATTT28980.10481926272778207No Hit
GTCCTACATCGTCGAAAGATCATCAGCTGTAAATGGAACGTGTTACCCTG28950.10471075417423363No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT28930.10463841513853468No Hit
GTATCAGAGCTTCAAGTCGATTAAAAATCACACTAAAGTTTGCTTTCAAT28880.10445756754928731No Hit
CTTCAATGGTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCTTA28770.10405970285294307No Hit
CACCTATACCGAGCAAACAAATTTGTACATAAGAAACGACACAACAACAA28730.10391502478154516No Hit
ATATAGTGGAGCGGATTCTGGAGGAAGAATCTGACGAGGCACTTAAAATG28500.10308312587100721No Hit
CCCTAAGAGGAAGAGGCAGCACTCTTGGTCTGGACATCAGAACTGCCACT28380.10264909165681349No Hit
GAACTGGGTGATGCCCCATTCCTTGACCGGCTTCGCCGAGATCAGAAGTC27980.10120231094283444No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)