FastQCFastQC Report
Mon 10 May 2021
HGJM2BGXJ_n02_ReSeq_2869_d10_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGJM2BGXJ_n02_ReSeq_2869_d10_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAATAAATTCTTCTCCAATGACCATATCCTGACTCCAATACGAGGATGTG11.2345679012345678No Hit
ATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCG11.2345679012345678No Hit
TCTGTAAAATATGCATAATCATCTCTGTCTCAGCGTTATGAAGTAAAAGG11.2345679012345678No Hit
ATGTAATATGGTGGTACCCTCATTTGTATTCTGCAGCAGAAAGGAACATT11.2345679012345678No Hit
GTCAAAAGTATGTGAATTCACAACTTACATGTAAGTCAAGTTCTACTTGT11.2345679012345678No Hit
CAGAAGGACAGGCCATGTGGCACATAGGTGGGAACAGGTGTCAGCATTCA11.2345679012345678No Hit
GATGCCTAACCAACTGAGCTAACGAGACACCCCATCTTCCCCGGACTCCA11.2345679012345678No Hit
ATATGCAGTGTGTAGATCTCGGTGGTCGCCGGATCATTATAAAAAGCCGG11.2345679012345678Illumina Single End PCR Primer 1 (96% over 29bp)
GGGCAACTAGGTGGCTCAGTCGATTGTCTGCTTTCACCTCAGGTCATGAT11.2345679012345678No Hit
GGCTAGACTCTGCACACGGCAGCGAGCCGATCCCTCCTGCTTGCTGCACA11.2345679012345678No Hit
CCCCTCCCTCTGTGTCCGTGGAGGCCACCTGGTAGCAATGTGTACCCTCC11.2345679012345678No Hit
GCACAGATAGATCACAGCCTGTGTGTCGTCAGGCTTCTCATTTATTTTGG11.2345679012345678No Hit
GTCAAGGGATTGGTTAATGATCATTCCCAATAATAAATTGACAGGGCACC11.2345679012345678No Hit
GCTCCGTCTTTCTAATTACTCAGTTACTCGAGACACACTGGGACAGGGAA11.2345679012345678No Hit
GATTCGTAGAATTCACAGGTGAAACCATGGGGTCCTGGACTTTTGCTTGT11.2345679012345678No Hit
GATGAGGGGACAGGGCAGGACCCTCAGGGCCCCATGGGATCATGGGCCAC11.2345679012345678No Hit
GTAGTGCCCTTCATTGGCTCTTGTTACAGAGTTTGGATTTTTTCTTGTTC11.2345679012345678No Hit
GTTGGCACAGGGAATGTTGTGATGAATAAGGCAGGTACAATCCTCTCATT11.2345679012345678No Hit
GTTTACAGATGAAGGAGCAATAGTGGGCGAAATCTAACCATTACCTTACC11.2345679012345678No Hit
GCATTCCTTCCTCTTGTTTTTCTGGCAGAGTTGGAGAAGGATTGGTATTC11.2345679012345678No Hit
CTTATCTATAAAGACTTTGTTATTTTAGCAACTGGAGCAAGCTTTCATCA11.2345679012345678No Hit
ATGCGTGTGGGTTTGTGTTCAAATTTCTTCTTATGACAACACCAATCACA11.2345679012345678No Hit
TCTCAGGCCTTTCGTGTCACCTGAGAACCTTAGACAAACACCCCCTGGCT11.2345679012345678No Hit
GGTGCTGGGAAAAGCGTGTCTTGGTGTGAGACTGAAATAAGTACAAGACA11.2345679012345678No Hit
GGGAATGGCCATCCTTTTAGCATTCATGATGGAAGACTGGGCCATCCCTC11.2345679012345678No Hit
ATTGAGTCCTGCATGAGGTCCCTGGTCAGCACGGGGAGCCCCTTTCTCCC11.2345679012345678No Hit
CCCCCTGCCTGTGCTCTCTCCGTCTCTCTCTCTCAAATAAATAAATAAAA11.2345679012345678No Hit
TGTCTTTACGGGCTATCTTAAGGAGTAGTCGTGAATTATGCTTAATGATT11.2345679012345678No Hit
GTACCCGCCATCCTCAAGGAATGGCTCAGTACACCCAGCACCGTTTCACA11.2345679012345678No Hit
CACAAAGGTTTTGCAATTTTTCTGTAACAGACCCAACCAATAGATTAAAA11.2345679012345678No Hit
GTGCTGGAATCCATAGCTTCCACGTTCTAATTTGAAGCAATCTGAACTCC11.2345679012345678No Hit
GCTCAATGAATTCCACTAAGGGTAAATATAAAAAATCGACTCTGATACAT11.2345679012345678No Hit
GTTCTTATTAACCCCATATACTGAATTTTTTAATTTTGAAAGAATGCTTT11.2345679012345678No Hit
GGGTGGGGGGCGGGGGGGGGGTGGGGGTGCGGGGGGGGGGGGGGGGGGGG11.2345679012345678No Hit
CTGTAATACCTAAAAACAATACAAATGTCCATCAATAACTGGATAAACAA11.2345679012345678No Hit
CTCCGGAAGGACCCTGACATACCCAAACGCATCCACAACCACTAACTGCA11.2345679012345678No Hit
GTAAAACAAAGATAATAAAAACCACCTGGGATAGTGCTCTAAGAACTGAA11.2345679012345678No Hit
TGTACGTGGTGGTCACGCGAGAAGGTGACTATGGAGACGTGAAAAGGTAT11.2345679012345678No Hit
GTGTGGGGACAGTGGAAAACAGATGTTCTTCCCAGCATACTCGTTTGCTA11.2345679012345678No Hit
GCTGTGTGGAGGGCAGCTCTGGGAAGGGCACCCCCCAGAGCTGGCCAGGG11.2345679012345678No Hit
GAGCAAAAGCAGGGGGAGTGGGAGAGGGAGAAGCAGACTCCCCGCTGAGC11.2345679012345678No Hit
GACCCTGAGATTATGACCTGAGCCAAAAGCAGAGGATTAACCCACTGAGC11.2345679012345678No Hit
TTGTTTATTTAAACAGGAAAAAAAAAAAGAAAAACATACTTAGAAAAGGT11.2345679012345678No Hit
AGAGAGAGCAGAGCGCGCACACATGCAAGCAGGGGGGAGGGAGAGAGGGA11.2345679012345678No Hit
GAGCAAAAGCAGGGTGGAGCCAGGCAAGCTGTTTTCTCTGAGCTGAAGGC11.2345679012345678No Hit
CATTTTTGATTTGTGGATGTTGAACCACCATTGCATCCCTGAAATACATC11.2345679012345678No Hit
GAAGGACAGGCCATGTGGCACATAGGTGGGAACAGGTGTCAGCATTCAAA11.2345679012345678No Hit
GGATCACATAGCACCGTGTTCAACCTTGAGAATTTCTGGTAACAACTCAT11.2345679012345678No Hit
AGTGAGAAGTGCTACGGTGCACGGGGACCCAGTGACTGCAGAAGAGCAGG11.2345679012345678No Hit
GTATGACACTGAGCGATGTGGTCTGTATCTGAGCCTGCACATCTCCTTCC11.2345679012345678No Hit
GAGCAAAAGCAGGGGGAGAGGGAGAAACAGGCTTCTTGCTGAGGAGGGAG11.2345679012345678No Hit
ATTTGGAGCCAACTGAAATCCTCAATGGCTTAGTACTGCTCGTGTGTATT11.2345679012345678No Hit
CCACAAGCAAATCTGTCACCAGTCACAAGGACGTGTGGCCAGGTGTTGAG11.2345679012345678No Hit
AAGCAGGAGAAGGGCGTGTTTGAGTTGTCACTGTTATTTGGAGATATGCT11.2345679012345678No Hit
GTATGAAGCTCGGCTGTTACATATATATTATGTTGCAATGGTTAGTTGTA11.2345679012345678No Hit
TTAGCTGAGTGTGCCAGGGAGCGGGAACAGTGTGGCGTGGGCAATTCTCT11.2345679012345678No Hit
CCACTGACTAATCGACTAGACTAATCAACTAATCGGGTGCCCCATTTTAG11.2345679012345678No Hit
GCCATGGGCAAGAGAAGTGTCACCGGCTCCCTGTGTGGAGAGATGGAAGC11.2345679012345678No Hit
GGAAAGTCACTGGCGGGCCACAGAACCACCAGCTGACCCCGGTGGCTTGT11.2345679012345678No Hit
GTTATGTGTTCGCCTGTGGCTCAAGGCCTCAATTTAGTGGCCTGGAAGTG11.2345679012345678No Hit
CATAATGGAAACATGGGCAAGAACTGGATCCTCCAGGTAGGGCTTGTAGG11.2345679012345678No Hit
AAAGCAGGGACAGAGAGAGGGAGGAGTCCTGGGGACTCTTCCAAAGGGAG11.2345679012345678No Hit
GTTCAAGGGAGTGGAGGTGTCAGGAGGACTCTCCAAGCTCCCAGAAAGAA11.2345679012345678No Hit
GTTAACACTTGTTAACATGAAAAACTGGGCTGAGAGAAACTGAATCTTTG11.2345679012345678No Hit
GGGATGTATTTATTGGTTTTGAGCCAGTGATGAGAGACACAAGTGGAAAA11.2345679012345678No Hit
GAAGCACAGGTCGGGGGGGCAGTGATTTGGTAGCAGCGCTATCAAATGCT11.2345679012345678No Hit
TTTATAAATATTTACACACCCAGCAGTGCAGATACTAAATATGTAGATCA11.2345679012345678No Hit
GGTACGTGATCCTACGGAAGTGTCCAGATAGTCACAAGGTTGCTGCAGCA11.2345679012345678No Hit
CTCCCTGCACTGAGCACCATTGTGCACCTCAGAGGCCTTCCGGGTGCTCC11.2345679012345678No Hit
GAGCAACTAAATATGTAGAGCATATATGACCAATCCTAAGAGAAGCAATC11.2345679012345678No Hit
GTATTTCTGTGTTCCTATTTTGGTTTGCCCACGAACCCAATTGGTGGTTC11.2345679012345678No Hit
GCTAAACAAGGACCCTGATTCGGGGCTTGATCCCAGGACTTTGGAATCAT11.2345679012345678No Hit
GTAAATACCATCAAATATATCCCTCGAGTGATACAAACAGCTGACGGGTA11.2345679012345678No Hit
TCTGAGTTCGGAATGGGGTCAGGTGGGACCACCGCGCTAAGGCCGCCAGG11.2345679012345678No Hit
ACACAGGAGTCAGCAGTCAGAAATATACCTTGCTGTCCTCGTGAGCCTTG11.2345679012345678No Hit
GATCTGATTACAGCTTCTCAAAATCTCGTTAAAAACCACCGGAAGAACAC11.2345679012345678No Hit
GTGATCCTATNATGTNNNNNNNNNNNAANNNANANGTNNTNNNTTANNNT11.2345679012345678No Hit
GGGGGAGGGAGATGAGTCAAATTTGCAGAATGACAGCTGTCAGGGCCCCA11.2345679012345678No Hit
CCCAAGACTCTGAGATCATGACCTGAGCTGAAGGCAGCGGCTTAACCCAC11.2345679012345678No Hit
GCAGTGCAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAA11.2345679012345678No Hit
GGCAGAGGGCATCTGCGGGAGGGCCACCTAGTGATGGATGGATCTGGGGG11.2345679012345678No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)