FastQCFastQC Report
Mon 10 May 2021
HGJM2BGXJ_n02_ReSeq_2251_d04_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGJM2BGXJ_n02_ReSeq_2251_d04_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1484285
Sequences flagged as poor quality0
Sequence length151
%GC43

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAGTGCAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAA62090.4183158894686667No Hit
GCATGGAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAAG51680.3481811107705057No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG48150.3243986161687277No Hit
ACCCCTTACTTGGATGGACTTGACAGATACACACAGAACCCTCCATCCAA45650.3075554896802164No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA40750.27454296176273424No Hit
GTAGCATGTTTTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG35960.2422715314107466No Hit
GTTTTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTGGAATTGA31420.21168441370761007No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC31010.20892214096349418No Hit
GGATCATTCCTTCCTCTTGTTTTTCTGGCAGAGTTGGAGAAGGATTGGTA27690.18655446898675118No Hit
CCCAACTGGTTGTTCAGTAGCATGTTTTTTAGTCTCTGTGTTTATGAATT25870.17429267290311495No Hit
CTTATATGGACTTGACAGATACACACAGAACCCTCCATCCAAAAGCAGCA24760.16681432474221597No Hit
CATCACCCTCAGACAACCCCTTACTTGGATGGACTTGACAGATACACACA24600.16573636464695124No Hit
GTTTTCTTCTTGTAATTGATTTCTAGTTCTATACCACTGTGGTTGGAAAA24240.1633109544326056No Hit
TTACTGCCCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA24040.16196350431352471No Hit
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT23970.1614918967718464No Hit
GTTGACATGGTCCTATGATGTTGATCCTTCGTTTTGTGAATGCGGCCCAT20400.13743991214625223No Hit
GCAGTGCAGCTACTAAATATGTAGAGCATATATGAACAAACCTAAGAAAA19980.13461026689618233No Hit
AAGTTGGAGAACGTTCTAGGGGCACTTGAGAGGAGTATATTCCTTTTGCT18930.1275361537710076No Hit
CTTCCCTCAATGATCCCCCATCCAGACAGAAGATCACAAAGGAAACATCA18870.12713191873528332No Hit
ATTTCCAGTTTTCTTCTTGTAATTGATTTCTAGTTCTATACCACTGTGGT18300.12329168589590273No Hit
ACCCCTTACTTGGATGGACTTGACAGATACACACAGAACTCTCCATCCAA17820.12005780561010856No Hit
CTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTCATT17570.11837349296125745No Hit
GCATGGAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAAAAG17220.11601545525286586No Hit
ACCTGTTACTTAGATGGACTTGACAGATACACACAGAACCCTCCATCCAA16860.11359004503852023No Hit
GTATTGGGAGTCAGGATATGGTCATTGGAGAAGAATTTATTGTGTTTATT16340.11008667472890987No Hit
GCTCTTAAGAATGTACTATGAACAATTACACCCCAACAAGCTGGATAATC15940.10739177449074808No Hit
AGTCTGTTCAGATGTCTTTTTCTTTAGGATTCAATCTTAGTAGGTTGTAT15460.10415789420495392No Hit
CTTCCCGTATTGTAGAATTTGGTTTGCAGATATTTGGGTGAGGATTTTTA15320.10321467912159726No Hit
GTTTTCTTCTTGGAATTGATTTCTAGTTCTATACCACTGTGGTTGGAAAA15240.10267569907396491No Hit
GTTTACTTCTTGTAATTGATTTCTAGTTCTATACCACTGTGGTTGGAAAA15190.10233883654419468No Hit
GCCCACGAACCCAATTGGTTGTTCAGTAGAATGTTGTTTAGTCTCTGTGT15060.10146299396679209No Hit
GTTGACATGGTCCTATGATGTTGATCCTTTGTTTTGTGAATGCGGCACAT14960.10078926890725164No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)