FastQCFastQC Report
Mon 10 May 2021
HGJM2BGXJ_n02_ReSeq_2241_d04_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGJM2BGXJ_n02_ReSeq_2241_d04_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1706284
Sequences flagged as poor quality0
Sequence length151
%GC44

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG31560.18496334725051633No Hit
GCAGTGCAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAA25220.14780657850627446No Hit
GTGTGGGGTTGAAACTCAAAACCACAAGATCAGGAGTCACAGAGCTCCAC23480.13760897951337528No Hit
ACCCCTTACTTGGATGGACTTGACAGATACACACAGAACCCTCCATCCAA22760.1333892833783825No Hit
GCATGGAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAAG22730.13321346270609113No Hit
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC20590.12067158808264042No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA20220.11850313312438024No Hit
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG19440.11393179564480474No Hit
GTATTGGGAGTCAGGATATGGTCATTGGAGAAGAATTTATTGTGTTTATT18460.10818832034995346No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)