FastQCFastQC Report
Mon 10 May 2021
HGJM2BGXJ_n02_ReSeq_2238_d12_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGJM2BGXJ_n02_ReSeq_2238_d12_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCACAGAGCTTGTCACAAGAGAGGCAGGATACAACCCCAGAGCCCAAAGG11.282051282051282No Hit
TCCATAACTGTCTCAAAAGTGACCATAAGCTCTCCTGAATCAGGAGAGAA11.282051282051282No Hit
GTGTGGGGCAGTGATTTGCTAGCGGCGCTAACAAATCCTTTTTATTCATC11.282051282051282No Hit
TTATAGCATTATTCAAGCCTTTAAAGTTAGCAGTTAAATGATGATCTAAA11.282051282051282No Hit
ATCCCGGAAGGACGTCCTGATGAGGTTTGCTTAAACATTATCAAATCGCA11.282051282051282No Hit
GAAACAGGGACCTGGGTTCATGACCTCAGACAAAACCAGACGCATAAAAA11.282051282051282No Hit
CTATCAATATCATTATAAATTATATTGAATTCTCTCTTCCTCCAAGATTT11.282051282051282No Hit
CAGTATCAATACCAAATAGTACCATTGACACAACTATTATAGTGCATGTG11.282051282051282No Hit
CCACTGAGCAGGCCATTTTAATGGGCGTCCGGGAATTCGCATCCAAAACA11.282051282051282No Hit
GTTAATAGTTGGCCATAGGCACAATTCAACAATTTAGGGTCCTGGAATTT11.282051282051282No Hit
AGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTA11.282051282051282No Hit
CCATATAGCCCCTCAAGATCAAAGCTTCCAGGTTCCAGGTTGTACATAAG11.282051282051282No Hit
ATTATTACCTGCAGACCATTGACAAGGGGCCTTGGCCCTCTCACTGGTTT11.282051282051282No Hit
CCCACCCGGCCTATCAACGTCGTCGTCTTCAACGTTCCTTAAGGAGACTC11.282051282051282No Hit
GCTTAGATGCTTTCAGCACTTATCTCTTCCGCATTTAGCTACCGGGCAGT11.282051282051282No Hit
TTCCAGACTCGAAGTCAATCCGTGCATCAATTCGGGCCCTAGACACCATG11.282051282051282No Hit
GTCCTCAAGCTCATAGAGACAGAGAAATAGAATGATGGTTACCAGGGACT11.282051282051282No Hit
GACTTAGAGTCTCCTGAAGGAACGTTGAAGACGACGACGTTGATAGGCCG11.282051282051282No Hit
GGTTAAGCCTCACGGGTCATTAGTACCGGTTAGCTCAACGCATCGCTGCG11.282051282051282No Hit
GCCATACTGACATCCCCTGAACCCAGAATGCACAGACGCCACCCCGTCAT11.282051282051282No Hit
CTATTGCACCGTTCGTGACAGTAAAGATGTGGTGAACACCACTGCGCAGG11.282051282051282No Hit
TCCTGAAGGAACGTTGAAGACGACGACGTTGATAGGCCGGGTGTGTAAGC11.282051282051282No Hit
GGCCAGCGCTCAACGGATTAAAGGTGATGCGGGGATAACAGGCTGATACA11.282051282051282No Hit
GTTCATAGATTGAGAACAAACCGATAAACATCAGAAAATCAATCAAATCA11.282051282051282No Hit
CTTTAAATATGATGGTTATACAAATTACAGACAGGTCAAATAAAATTAAA11.282051282051282No Hit
TTCCTGGTTGTGTCGGGGAAGATTTGGATTCTTTGGTAGGAACTAGCGGA11.282051282051282No Hit
GTATACTTAAAACGGGTGCGTTTTATGGCGTGTTCAACCCACCTTTAAAC11.282051282051282No Hit
TCGTGGAGCTTGGATGGACTGAGCATTCCTGAGCCCAGCAGGTGTCCTGG11.282051282051282No Hit
ATCCTAGGGAAATAATTAAGGACACACAAGGACACTATTCTTGGTGTTCT11.282051282051282No Hit
TATCAACAGTGATGGGGTCCTGGATGAACAGGAGCTGGAGGCCCTCTTCA11.282051282051282No Hit
AGTTCTAACTATAATCCCAACATGCACCCTAAAATACGAGATCGCGAAGA11.282051282051282No Hit
GGCTAGGCAAATGGTGCAGGCTATGAGGACAATTGGGACTCACCCTAGTT11.282051282051282No Hit
GATTAAATCAGAACGCAGAAGCGGTCTGATAAACAGAATTTGCCTGGCGG11.282051282051282No Hit
GGCACCCTGCGGCTCAGACGTTCATAACAACGTTTTATTACGGGATCCCG11.282051282051282No Hit
GTATCATTTTCAACACATTCTGTTGACCAAAAGGAAAGTGTTAGGGCCCA11.282051282051282No Hit
GTCCCCAAGGTGGCTTCTGCAGAGCACCCAGCAGGTGGGCTCTGTGGAGT11.282051282051282No Hit
ACCCATAACCTTCAGTTATAGATTAAATGTTTGTGCCCACCCCCAAATCC11.282051282051282No Hit
CCTGTAGAATGATGCTGATCATGGTCTGCTTGGTCCTGTGGATGTAACAT11.282051282051282No Hit
ATAATAGAATTGACAAATATAGAGAAAATAAATCCAAATCTTTAAAAATA11.282051282051282No Hit
GTTCCCGGCTACTACCGAGTTCAGTCCTGCACGTGGGAATGCAAGAAACT11.282051282051282No Hit
AACAGTTAAACTGACTGAGCCACCAAGGCATACCAGCAAATCAATTTAGA11.282051282051282No Hit
CTCTCTATCATCCCATCAGGCCCCCACAAAGCCGAGAACGCGCAGAGACT11.282051282051282No Hit
CGTGTCCAATGGTCGTCTCTCATACGATGAATGGTAAAGAAACTAAACGA11.282051282051282No Hit
GTATTACTCAGTCTGCTGCTGTTGTATGTTATGTCAGGCATTTTACTGAT11.282051282051282No Hit
CTCGAATACCGCCTTCACGTTTATGTCCAGACTACATATCTCTCTAAGCA11.282051282051282No Hit
ATAAAAAACTACAAATCATCAAGACACCACAATAAAAACGAACTAATAAT11.282051282051282No Hit
GTTTCAGGCTGAAAATCTTCTCTCATCCGCCAAAACATCTTCGGCGTTGT11.282051282051282No Hit
CTCTGATAACATACCAAACAAGTCATCCGATCCGCACCACAATTGCAATA11.282051282051282No Hit
GACCGATGCATGGCTTGATACAATGAAACTTAGATCATGATCTGAGACAA11.282051282051282No Hit
GAATACATATACTGGAACTGCATAAGTAAAATGCCTGACATAACATACAA11.282051282051282No Hit
CTCCTGATCTTGTGGTTTTTAGTTTCAACCCCACACTGGGCATAGAGCTC11.282051282051282No Hit
GTGGTACTCAGGGATAGTGCGGTCCTGGGATTGTTTAAGCAGAAATAATC11.282051282051282No Hit
GGAGCATTCCTTACTCTTGTTTTTCTGGCAGAGTTGGAGAAGGATTGGTA11.282051282051282No Hit
GACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCAT11.282051282051282No Hit
CAACAAATATACCTTCTTGAGATCACACTCTAGCTCTGTCTATCAGTAAA11.282051282051282No Hit
ATATACAACCGGATGAGAACAGTGACCACATAATTGTCTATTTGCCTAGT11.282051282051282No Hit
CCGCGCTAAGGCCGCCAGGCAAATTCTGTTTCATTAACCCGCTTGTCGCC11.282051282051282No Hit
GAGCACATTGCCTGATGCGACGCTGCGCGTTTTATCAGGCCTCCGGTGAT11.282051282051282No Hit
CATAAAGGGTGTAGTCAGCTTTACTGGCCATTTCTTCTCAAGTGAATGAA11.282051282051282No Hit
GGTATTAATTGATATGCAAAAAACCCCGGCCTTATTGACCGGGGTTTTTC11.282051282051282No Hit
GCTATACCCCCAACATACAGTATTAAAAGGTAAATAACATCCAGTGAGTG11.282051282051282No Hit
AAACATGGTTGTCAACAGTAAAATTCCTTCTATAAAGGACCTCATATCTA11.282051282051282No Hit
GTGCTAAGCTTGGCACAGGGGCGCGCTGGGGATGTCCCAGGGCCGCCAAC11.282051282051282No Hit
GAGTGAACAACGACCAGGACTAGCTGGTAATCCACGGATAGCTGCCCGCC11.282051282051282No Hit
GATTTAATCTGTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACATCT11.282051282051282No Hit
GGCCCTTACAACCAGGATTTATCAGATCCTTTGGAACTCCTCTTCTGCGC11.282051282051282No Hit
GTCGCAAGTCAGGCTAGGCAAAAGGTGCAGGCTATGAGGACAATTGGGAC11.282051282051282No Hit
CACTAGAACAATTGCAGGACTAAAAGATGATATTAATTAAAATTTTCAGT11.282051282051282No Hit
CTGTGGGAGCATAATTTTAACCCTTGCCTCCATCCCACATCGCCCTTTCC11.282051282051282No Hit
GAATAAAGGAGTAGTGGATGGAAGGGGAGGGACGTGGCTTTGATGCCAGA11.282051282051282No Hit
CTAATCATGAATGATAACATAATGTTAATAGACATAACAACACCTAAGAA11.282051282051282No Hit
GAATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGATAACAG11.282051282051282No Hit
GTTTGAAGCTTATAGCAAGTTTATTGGGCATGAACAGCACGTTGCGCTGG11.282051282051282No Hit
GATCCTAGCTGTGGAGGGCAGATCGAACCACCAGACATCTTCCGGACACT11.282051282051282No Hit
GGGATCATTCCTTCCTCTTGTTTTTCTGGCAGAGTTGGAGAAGGATTGGT11.282051282051282No Hit
CTTGTAGTATAAATCAAGTTCTAAACAACAAAGGTTCGAAAAGATGGTAT11.282051282051282No Hit
CTTTTATGTGCTGGATTCTCATTTGGTCTAATAAGACTGAAGACCTGACT11.282051282051282No Hit
ACCAAAGATTTCTAATGACATTCTTCGTGACTAGGATACTAAGATCACAT11.282051282051282No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)