FastQCFastQC Report
Mon 10 May 2021
HGJM2BGXJ_n02_ReSeq_2232_d04_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGJM2BGXJ_n02_ReSeq_2232_d04_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCCACTTAGCTTACCAACATTTTATATAAAGATTCAACTGAAGTATCA14.545454545454546No Hit
GGCTCGAGCAGGGGAGGGGTAGAAAGAGAGGGAGAGAGAGAATCTCAAGC14.545454545454546No Hit
GCTGGGGGCCTTGAGGTGACACTCTTGCGGAGAGGAAGGACAGCATCCGT14.545454545454546No Hit
GAAAGAGGAGTGTAAACGTTGATAGGTCCCTACATTCTCAAACCCTCCCA14.545454545454546No Hit
AGCCGGGACTAGGGCCCCGTGAGGCGTAGCCTGACAGCGCCGCAGCTGTG14.545454545454546No Hit
AATCTATTCTCTAAAGCATTGTTTTCTTGCTGGAATATTTTGAAGCAATC14.545454545454546No Hit
TTAGAACACTGTGTCGAGGTTTCAGGTAGACTGCTTTCTTTGGCATGTCC14.545454545454546No Hit
CCTTTATTCTTCACCATTATATTAAAAAGAAAATAATGAATTACACTAAA14.545454545454546No Hit
AGATTGAATCCTAAACAAACAGAAATCTGAAAAGACAAATGAGAAGTAGA14.545454545454546No Hit
ATCCACAGCATTCTGCTGTTACTGCCGATACTCTTCCCTCATAGACTCAG14.545454545454546No Hit
GTTGTTAACTAGGAATTGAAATACTCATTCCTTGGGGTGGAGATTTCTTG14.545454545454546No Hit
AAAAAAATACAATATCTGAATTGGAAAAGCCACTGCAGGGCCTTAACAGA14.545454545454546No Hit
AAATAAAATCTTATGAATAAACAAATAGGAAAGCAACATTCTAACAACAA14.545454545454546No Hit
TTTGGAGGCAGAGGAGATCTCCCATACTCATCACTGCTTCTCCAAGTGAA14.545454545454546No Hit
TATTTTCTTCTGTCTTTCCTTCCTTCTGGATTCCAGTTATACCAAATGAT14.545454545454546No Hit
GGGGTGGGGAGTAGATCAGTCTACCCAGAGTCAAGGGTTTGTTTAGGGAA14.545454545454546No Hit
ATACTGAGGACCCAGATGAAGGAACAGCAGGAGTAGAGTCTGCAGTATTG14.545454545454546No Hit
GATATGAACTTCTAGGGAAGAATTACTCGAATAGAGTCAACTTCTGGTAC14.545454545454546No Hit
GTCATACCGCGTCAGCTCTCTCACCCATTTCCCGTATCTCCAGCGGTAGA14.545454545454546No Hit
AGATCATTCTTGGTCACACAACATCATCGCATGTAGAACTAGATCCGAAG14.545454545454546No Hit
GGATGGCATTTCAATAAAATCGAAATAATCCTGATAAATTCTTATAAAGA14.545454545454546No Hit
GTGGATTGAAAATGCCGTGCGCGGCCCCTTTGATCATCAATGTCTGATTT14.545454545454546No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)