FastQCFastQC Report
Mon 10 May 2021
HGJM2BGXJ_n02_ReSeq_2231_d04_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGJM2BGXJ_n02_ReSeq_2231_d04_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1319715
Sequences flagged as poor quality0
Sequence length151
%GC50

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGACGGATAAGCAAAAGTAGGCTGGATAAGACGCGCCAG142201.0775053704777169No Hit
GAGCAAAAGCAGGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATC96300.7297030040576943No Hit
GGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGA91730.6950743152877705No Hit
GGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACG90870.6885577567883975No Hit
GGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGAT74070.5612575442425068No Hit
GTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACATCTTCGGCGTTGT73290.5553471772314477No Hit
GGATAAGACGCGCCAGCGTCGCATCCGGTATTAATTGATATGCAAAAAAC69680.527992786321289No Hit
TCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGG65800.49859249913807147No Hit
GTATTAATTGATATGCAAAAAACCCCGGCCTTATTGACCGGGGTTTTTCT58470.4430502040213228No Hit
GGGTCAGGTGGGACCACCGCGCTAAGGCCGCCAGGCAAATTCTGTTTCAT58400.4425197864690482No Hit
GTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGC53640.40645139291437926No Hit
CATTAGTACCGGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGCCTA52930.40107144345559465No Hit
GGTGGGACCACCGCGCTAAGGCCGCCAGGCAAATTCTGTTTCATTAACCC52520.39796471207798656No Hit
GCAAAAGCAGGACGGATAAGCAAAAGTAGGCTGGATAAGACGCGCCAGCG49710.37667223605096556No Hit
AATCTAATCTGTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACATCT49660.37629336637076943No Hit
CGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTG47010.356213273320376No Hit
GTGTAAGCGCAGCGATGCGTTGAGCTAACCGGTACTAATGAACCGTGAGG43590.3302985871949625No Hit
GGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCG42440.3215845845504522No Hit
CGCTAAGGCCGCCAGGCAAATTCTGTTTCATTAACCCGCTTGTCGCCGGT40570.30741485851111794No Hit
GTACTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGATGTTTTGGC40040.3033988399010393No Hit
GTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTT39780.3014287175640195No Hit
TTGTAAGGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCAACGCATC37850.28680434790844994No Hit
AATCTGTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACATCTTCGGC37300.28263678142629284No Hit
GAGTGGACGCATCACTGGTGTTCGGGTTGTCATGCCAATGGCACTGCCCG37010.2804393372811554No Hit
GACTTAGAGTCTCCTGAAGGAACGTTGAAGACGACGACGTTGATAGGCCG35940.2723315261249588No Hit
CATCACTGGTGTTCGGGTTGTCATGCCAATGGCACTGCCCGGTAGCTAAA35610.26983098623566454No Hit
ACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATG35560.2694521165554684No Hit
GTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGA35130.26619383730578194No Hit
ACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTG34840.2639963931606445No Hit
TAGTACGAGAGGACCGGAGTGGACGCATCACTGGTGTTCGGGTTGTCATG34400.26066233997491883No Hit
AAGCAGGACGGATAAGCAAAAGTAGGCTGGATAAGACGCGCCAGCGTCGC34370.2604350181668012No Hit
CTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGATGTTTTGGCGGA34340.2602076963586835No Hit
CTCTAAGTCTCAGGGAGAACTCATCTCGGGGCAAGTTTCGTGCTTAGATG33540.25414578147554584No Hit
TCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGT32910.24937202350507498No Hit
GTGCTGAAAGCATCTAAGCACGAAACTTGCCCCGAGATGAGTTCTCCCTG31680.24005182937225084No Hit
GTTTGGCACCTCGATGTCGGCTCATCACATCCTGGGGCTGAAGTAGGTCC31100.23565694108197605No Hit
TCACTGGTGTTCGGGTTGTCATGCCAATGGCACTGCCCGGTAGCTAAATG30560.2315651485358581No Hit
GCAAAAGCAGGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCGC30310.2296708001348776No Hit
TCATTAACCCGCTTGTCGCCGGTTAATCTAATCTGTATCAGGCTGAAAAT30300.22959502619883837No Hit
TCCTGGGGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGT30180.22868573896636774No Hit
GGAGTGGACGCATCACTGGTGTTCGGGTTGTCATGCCAATGGCACTGCCC29590.22421507674005373No Hit
GCCTTATTGACCGGGGTTTTTCTAATTTGATGCCTGGCAGTTCCCTACTC29230.2214872150426418No Hit
GTCATGCCAATGGCACTGCCCGGTAGCTAAATGCGGAAGAGATAAGTGCT29170.22103257142640645No Hit
CAATGGCACTGCCCGGTAGCTAAATGCGGAAGAGATAAGTGCTGAAAGCA28940.21928977089750437No Hit
ATCTAATCTGTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACATCTT28100.21292476027020987No Hit
TCGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCA27550.20875719378805271No Hit
AGCGAAAGCAGGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCG27240.20640820177083688No Hit
CTTACACACCCGGCCTATCAACGTCGTCGTCTTCAACGTTCCTTCAGGAG27190.20602933209064078No Hit
GATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATA27050.20496849698609168No Hit
GAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAG26400.20004319114354235No Hit
GTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCTAGTA26300.19928545178315016No Hit
CTATTGCACCGTTCGTGACAGTAAAGATGTGGTAAACACCACTGAGCAGG25990.1969364597659343No Hit
GTTCTGAATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGAT25950.19663336402177745No Hit
GGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGCCTATCAACGTCGT25550.1936024065802086No Hit
ATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGT25230.19117764062695353No Hit
GCCCGGTAGCTAAATGCGGAAGAGATAAGTGCTGAAAGCATCTAAGCACG24940.18898019648181616No Hit
GGTCAGGTGGGACCACCGCGCTAAGGCCGCCAGGCAAATTCTGTTTCATT24580.1862523347844042No Hit
CTCCTGAAGGAACGTTGAAGACGACGACGTTGATAGGCCGGGTGTGTAAG24510.18572191723212966No Hit
GTGTTCGGGTTGTCATGCCAATGGCACTGCCCGGTAGCTAAATGCGGAAG24280.18397911670322759No Hit
TTGATAGGCCGGGTGTGTAAGCGCAGCGATGCGTTGAGCTAACCGGTACT23690.17950845447691358No Hit
GCCTATCAACGTCGTCGTCTTCAACGTTCCTTCAGGAGACTCTAAGTCTC23580.17867494118048216No Hit
GAGCAAAAGCAGGTCGCGGCGTATATTTTGGCGGCTCAGATTTTGCCTGC23100.17503779225059957No Hit
CCCTGAGACTTAGAGTCTCCTGAAGGAACGTTGAAGACGACGACGTTGAT22960.17397695714605046No Hit
AGCGAAAGCAGGACGGATAAGCAAAAGTAGGCTGGATAAGACGCGCCAGC22830.1729918959775406No Hit
AGCAAAAGCAGGACGGATAAGCAAAAGTAGGCTGGATAAGACGCGCCAGC22580.1710975475765601No Hit
GCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCAC22480.17033980821616787No Hit
CGCTTACACACCCGGCCTATCAACGTCGTCGTCTTCAACGTTCCTTCAGG22340.1692789731116188No Hit
CCTAGTACGAGAGGACCGGAGTGGACGCATCACTGGTGTTCGGGTTGTCA22300.16897587736746192No Hit
GTACGAGAGGACCGGAGTGGACGCATCACTGGTGTTCGGGTTGTCATGCC22000.1667026592862853No Hit
GAATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGATAACAG21850.16556605024569698No Hit
GTACCGGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGCCTATCAAC21780.16503563269342245No Hit
ATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGATAAAA21730.16465676301322635No Hit
TATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCTAGTACGAGA21710.1645052151411479No Hit
CATCACATCCTGGGGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCAT21090.1598072311067162No Hit
GAGTAATACAGTGCTATCAAGCTGTTCAAGTGCCATACTGACATACCCTG21070.15965568323463777No Hit
CCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGG20770.15738246515346116No Hit
GTTCGGTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCC20720.15700359547326506No Hit
GAGCAAAAGCAGGTTCTGAAAGTGAGTATGCAAAGCAATGCACCTGTGCC20500.1553365688804022No Hit
GTCTCAGGGAGAACTCATCTCGGGGCAAGTTTCGTGCTTAGATGCTTTCA20290.1537453162235786No Hit
CTTCAGGAGACTCTAAGTCTCAGGGAGAACTCATCTCGGGGCAAGTTTCG20260.1535179944154609No Hit
GTTGTAAGGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCAACGCAT19920.15094168059012741No Hit
GGGTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTG19590.14844114070083314No Hit
CCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGG19580.14836536676479392No Hit
GTGTGTAAGCGCAGCGATGCGTTGAGCTAACCGGTACTAATGAACCGTGA19480.1476076274044017No Hit
CTGGAGAACTGAGGGGGGCTGCTCCTAGTACGAGAGGACCGGAGTGGACG19190.1454101832592643No Hit
GAGGGGGGCTGCTCCTAGTACGAGAGGACCGGAGTGGACGCATCACTGGT19060.14442512209075442No Hit
GTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC18680.14154571252126405No Hit
GCGCTTACACACCCGGCCTATCAACGTCGTCGTCTTCAACGTTCCTTCAG18560.14063642528879342No Hit
TTTCAGCACTTATCTCTTCCGCATTTAGCTACCGGGCAGTGCCATTGGCA18470.1399544598644404No Hit
CCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTT18280.13851475507969524No Hit
ACCTTACAACGCCGAAGATGTTTTGGCGGATGAGAGAAGATTTTCAGCCT18240.13821165933553836No Hit
AAAGTAGGCTGGATAAGACGCGCCAGCGTCGCATCCGGTATTAATTGATA17990.13631731093455784No Hit
CACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCA17290.1310131354118124No Hit
GACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCAT17070.12934610881894956No Hit
TCCTTCAGGAGACTCTAAGTCTCAGGGAGAACTCATCTCGGGGCAAGTTT17020.12896723913875346No Hit
CTCCTAGTACGAGAGGACCGGAGTGGACGCATCACTGGTGTTCGGGTTGT17000.128815691266675No Hit
GCTCAACGCATCGCTGCGCTTACACACCCGGCCTATCAACGTCGTCGTCT16800.12730021254589058No Hit
AGTACGAGAGGACCGGAGTGGACGCATCACTGGTGTTCGGGTTGTCATGC16530.12525431627283162No Hit
GCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCA16470.1247996726565963No Hit
GAGCTAACCGGTACTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAG16440.12457235084847865No Hit
GTATTACTCAGTCTGCTGCTGTTGTATGTTATGTCAGGCATTTTACTGAT16440.12457235084847865No Hit
GGGCTGCTCCTAGTACGAGAGGACCGGAGTGGACGCATCACTGGTGTTCG16080.12184448915106671No Hit
GCTCCTAGTACGAGAGGACCGGAGTGGACGCATCACTGGTGTTCGGGTTG15760.11941972319781166No Hit
GCCTGATACAGATTAGATTAACCGGCGACAAGCGGGTTAATGAAACAGAA15630.1184346620293018No Hit
GTAGCTAAATGCGGAAGAGATAAGTGCTGAAAGCATCTAAGCACGAAACT15620.11835888809326256No Hit
CCTGAAGGAACGTTGAAGACGACGACGTTGATAGGCCGGGTGTGTAAGCG15570.11798001841306646No Hit
GATTAGATTAACCGGCGACAAGCGGGTTAATGAAACAGAATTTGCCTGGC15390.11661608756436048No Hit
TTCCATAACTGTCTCAAAAGTGACCATAAGCTCTCCTGAATCAGGAGAGC15370.11646453969228206No Hit
GGGGTCAGGTGGGACCACCGCGCTAAGGCCGCCAGGCAAATTCTGTTTCA15350.1163129918202036No Hit
GCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCTAGTACGAGAGGACC15310.11600989607604673No Hit
GATAAGTGCTGAAAGCATCTAAGCACGAAACTTGCCCCGAGATGAGTTCT15290.11585834820396829No Hit
GGCTTAACCTTACAACGCCGAAGATGTTTTGGCGGATGAGAGAAGATTTT14940.11320626044259556No Hit
AACCGGTACTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGATGTT14940.11320626044259556No Hit
CTATTGCACCGTTCGTGACACAGACTCGGGATCAATTTAGCTTTGTTGAG14860.11260006895428179No Hit
GTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGGGCTGAAGTAGGT14790.11206965140200727No Hit
AGCAAAAGTAGGCTGGATAAGACGCGCCAGCGTCGCATCCGGTATTAATT14780.11199387746596803No Hit
ATCACATCCTGGGGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATT14550.11025107693706596No Hit
TGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTT14510.10994798119290908No Hit
GATAAGCAAAAGTAGGCTGGATAAGACGCGCCAGCGTCGCATCCGGTATT14320.10850827640816389No Hit
ACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCAC14060.10653815407114416No Hit
GGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCG14050.10646238013510494No Hit
CCTTCAGGAGACTCTAAGTCTCAGGGAGAACTCATCTCGGGGCAAGTTTC14010.10615928439094803No Hit
CTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGG13960.10578041471075193No Hit
CTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTAAGGCCGCCA13880.10517422322243816No Hit
GCATGGGGTCAGGTGGGACCACCGCGCTAAGGCCGCCAGGCAAATTCTGT13860.10502267535035974No Hit
GGTTAATCTAATCTGTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAAC13850.10494690141432052No Hit
AGGTGGGACCACCGCGCTAAGGCCGCCAGGCAAATTCTGTTTCATTAACC13790.1044922577980852No Hit
AAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGAC13780.10441648386204597No Hit
GGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTT13770.10434070992600675No Hit
AGCAAAAGCAGGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCG13610.10312832694937923No Hit
GTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTC13590.10297677907730078No Hit
CATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAG13590.10297677907730078No Hit
TACACACCCGGCCTATCAACGTCGTCGTCTTCAACGTTCCTTCAGGAGAC13520.10244636152502624No Hit
GACCGGAGTGGACGCATCACTGGTGTTCGGGTTGTCATGCCAATGGCACT13450.1019159439727517No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)