FastQCFastQC Report
Mon 10 May 2021
HGJM2BGXJ_n02_ReSeq_1971_d04_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGJM2BGXJ_n02_ReSeq_1971_d04_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1303806
Sequences flagged as poor quality0
Sequence length151
%GC51

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATATTACAACCAGCAAAGCCCTCATCCCCAACAGCAGCAGCCTCCATA58740.45052714897768537No Hit
GGGAATATGCAGGCTGCTGCTGCTGCGGCTGTGACTGAGGGTATGAAGAT57460.4407097374916207No Hit
CTCCTAAAGAGCACAGCTCAAGTCCTGGTTGGTGGCACACTTGCAAGATT51960.3985255475124367No Hit
GGAATATGCAGGCTGCTGCTGCTGCGGCTGTGACTGAGGGTATGAAGATG51040.3914692830068277No Hit
GTACTAACACGTGGCTCTTTTTCTGCACAGGGGAAGGGGCTGCAGTCTTA45560.3494384900821134No Hit
ATTCTAGCCTGCCCCATGAATAAAATTCCACCTTGCTGCAATAAAGTATG42800.3282696965652866No Hit
GAATATGCAGGCTGCTGCTGCTGCGGCTGTGACTGAGGGTATGAAGATGA41140.3155377410442965No Hit
CTCGTATGCTCAGCAGATTCCACCTTATGGACAGCAGGGTCCCAGTGCAT38540.29559612396322765No Hit
ATGCAGGCTGCTGCTGCTGCGGCTGTGACTGAGGGTATGAAGATGACTGC37360.2865456977495118No Hit
CTGTAAGGGCAGAGCAAGTCCCAGACCAACTCATGAGACTGAATTTGGAC36690.28140689642477484No Hit
ATCCCAGACCCCTCACTCTCAGCCTTCTTATCAGCAGCAGCAACAGCCTC36600.28071660967966094No Hit
GAGTAGGGAGGCTGATTCTGTTGGTGCTGCTGGGCTGTGGACTGCTGCTG36180.27749527153579595No Hit
GTCCCAGTGCATATGGGCAGCAAAGCCAGACTCCATATTACAACCAGCAA35630.2732768525378776No Hit
GACTGAGGGTATGAAGATGACTGCTGAGAAAGAGCCGAGGAAGCTGTCTG35560.2727399628472334No Hit
GTGCATATGGGCAGCAAAGCCAGACTCCATATTACAACCAGCAAAGCCCT32970.25287504429339946No Hit
GTACAATGGGCAGCATCTCGTATGCTCAGCAGATTCCACCTTATGGACAG32950.2527216472389297No Hit
GTCCAGGTACCATCCAGATGGCTGCATCTTGAGTCAGGTCCCTTTGGTCA32810.2516478678576414No Hit
GTATGAAGATGACTGCTGAGAAAGAGCCGAGGAAGCTGTCTGCTGAGTTT32420.24865662529548108No Hit
ATCCAAAGCTGCTCCCAAACCCAGTGGATTTATAATAAACTCTGATGATA32320.2478896400231323No Hit
TGTTAGTACAGGTTTGTTCAGAAGGAACATCTCAGCAGAAGCACTTCAGT32270.24750614738695786No Hit
GTTCTCCATCATATTTGAAAAGTCGTGGCTGTCAGGCAAAGTTCCTGGGG29670.22756453030588908No Hit
GTCCTAAAGGAGCTGGCTGATGTGGTTGCTGAGCTGTTCTCCATCATATT29440.22580046417948682No Hit
GTCTTACTCCGCTCTCTGGAGCCTCTCCAGGGTTTGGATGTGATGAGGGA29390.22541697154331242No Hit
GGGTATGAAGATGACTGCTGAGAAAGAGCCGAGGAAGCTGTCTGCTGAGT29230.22418979510755435No Hit
GAATATAGGGGACCATGTTAAAGGCCTTGCAGAAGTTCAGGTGGATGACA28710.22020147169134058No Hit
GCTTTGGATAGTTATGTCAAAACTGAACCATGTGTGTATTCATTCCTCAA27150.2082365014426993No Hit
CTGCTGGCTCATGTTAAGTTTTTGATCAACCAGAACCTTAAGTCCTTCTC26240.20125693546432524No Hit
GGGGAAGGGGCTGCAGTCTTACTCCGCTCTCTGGAGCCTCTCCAGGGTTT25620.19650162677576263No Hit
GTCTACAACACTCACAAATTTGTTTCCCCGTAAATAAAACCTCAGAGGTT25590.196271531194058No Hit
TCCATACTCTCAGCAGACACCATCCCAGACCCCTCACTCTCAGCCTTCTT25510.195657942976179No Hit
GTTAAAGGCCTTGCAGAAGTTCAGGTGGATGACATCAGATGCCTTCCCTT25300.1940472739042465No Hit
GCAATAAAGTATGTTTATTGTTAAAAACCTCCAAGCTGCCCTTGCTGCTG25130.19274339894125353No Hit
ATCATAAAATGGCATCAGATTGGTCAGGCATGATCTTCCCTTGGTGAAGC24780.19005895048803273No Hit
GTGTAATGGGCCCTCCAAGCTCTGCCAAGCATTTGGGATTGATAAGGCTT24740.1897521563790932No Hit
CCTATATTCTTATCTCTAAATTGGAGAGAGAGGGATTTGAAAGATGGACT24550.18829488436163047No Hit
TCCCAATACCAATCCTGGTCGTGGCTACCACGTCCTGCTCCCCTGGCAGC23890.1832327815641284No Hit
GCTGATAGGGTGACTGTGACTGCTGCTGTGAGTAGGGAGGCTGATTCTGT23700.1817755095466657No Hit
GTACCTGGACAGGAGCTGCCAGGGGAGCAGGACGTGGTAGCCACGACCAG23670.18154541396496104No Hit
GAGTAAGACTGCAGCCCCTTCCCCTGTGCAGAAAAAGAGCCACGTGTTAG23510.18031823752920295No Hit
GAAGATGACTGCTGAGAAAGAGCCGAGGAAGCTGTCTGCTGAGTTTGCTG23180.17778718613045194No Hit
CTTATGGACAGCAGGGTCCCAGTGCATATGGGCAGCAAAGCCAGACTCCA23110.17725029643980778No Hit
CTCTCAGCCTTCTTATCAGCAGCAGCAACAGCCTCAGTCTCAAGCTCCAC23050.1767901052763985No Hit
ATAATAAACTCTGATGATAAGGTCTCCAGCCAGCAAATGAGAGGAGAAAG22720.17425905387764745No Hit
CTCTGGAGCCTCTCCAGGGTTTGGATGTGATGAGGGAGCTGCGCAGCACT22490.1724949877512452No Hit
GATATGGAGAAGGCTGAGGTACTCAATGAGTTCTTAGCCTGAGGCTGGCT22330.1712678113154871No Hit
ATATTACAACCAGCAAAGCCCTCATCCCCAACAGCAGCAGCCTCCATACT22190.1701940319341988No Hit
TCCCTGAACCTCACATCCCTGAACCTGAGCCTCCAGGTGGGGAACTGCCC22150.16988723782525927No Hit
TTCCTCAACATCGTGGTGATATAAGAAAAACCTCTTGCCCTCTGGCAAGC21850.16758628200821288No Hit
GTATCACCCAAGGCCGTGTTTGTCACACCCACACCTCAGCCTGTCACTTG21820.16735618642650824No Hit
CCTTATGGACAGCAGGGTCCCAGTGCATATGGGCAGCAAAGCCAGACTCC21410.16421154680987815No Hit
CCTCAACATCGTGGTGATATAAGAAAAACCTCTTGCCCTCTGGCAAGCCT20980.16091351013877833No Hit
GGTCAAAGCAGTGACCCTTCACTAGTCATCCACCTTACCAGAGCCTGGAT20920.16045331897536905No Hit
CCTCTGTATCACCCAAGGCCGTGTTTGTCACACCCACACCTCAGCCTGTC20770.15930284106684584No Hit
ATTACAACCAGCAAAGCCCTCATCCCCAACAGCAGCAGCCTCCATACTCT20550.15761547346767846No Hit
TCTTCCAGGAGAGCTGTTCCATGATCTTCCTGGGCACAGAGGTGAGGCTC20170.15470092943275301No Hit
GCATAGGGAAGGGAGTGCACTGACCACGGGTTAGCAGCCTGAGGGAAGCA19870.15239997361570665No Hit
TCCTTGAAGTGCTGCGCAGCTCCCTCATCACATCCAAACCCTGGAGAGGC19580.1501757163258951No Hit
GTGCAGAACCTTGCATTTTGCTTAGTTGAATCTCATTAGTTCCACACGGG19270.14779806198161383No Hit
TCCCAGACCCCTCACTCTCAGCCTTCTTATCAGCAGCAGCAACAGCCTCA18850.14457672383774886No Hit
ATTCAGTGGATAAGGAATCGGTTGGAAGGTCACAGCCAAAGGCTTGTGGT18790.1441165326743396No Hit
AGCGAAAGCAGGGAATATGCAGGCTGCTGCTGCTGCGGCTGTGACTGAGG18620.14281265771134663No Hit
GTTAAAAACCTCCAAGCTGCCCTTGCTGCTGCCCCAACATGGCTTGAGAC18450.14150878274835368No Hit
TATGCAGGCTGCTGCTGCTGCGGCTGTGACTGAGGGTATGAAGATGACTG18210.13966801809471655No Hit
GTGTGTATTCATTCCTCAACATCGTGGTGATATAAGAAAAACCTCTTGCC18190.13951462104024678No Hit
GTACAGGTTTGTTCAGAAGGAACATCTCAGCAGAAGCACTTCAGTTGCAT18160.13928452545854214No Hit
GTAATGGGCCCTCCAAGCTCTGCCAAGCATTTGGGATTGATAAGGCTTTT18070.13859423871342824No Hit
GGGAAGGGAGTGCACTGACCACGGGTTAGCAGCCTGAGGGAAGCACAGGC17980.1379039519683143No Hit
CTTCAAGGAAAGGACCTGCAAAGCCACTGAAGGACTGGCAGCTGTGTAAT17960.13775055491384455No Hit
TTCCAGGAGAGCTGTTCCATGATCTTCCTGGGCACAGAGGTGAGGCTCAC17720.13590979026020744No Hit
GGCTGTGACTGAGGGTATGAAGATGACTGCTGAGAAAGAGCCGAGGAAGC17600.13498940793338884No Hit
AAAGCAGGGTGATGAATCACACTGGAGAAGAAAACAGGAGTTTTCTTCAC17360.13314864327975173No Hit
TACCAATCCTGGTCGTGGCTACCACGTCCTGCTCCCCTGGCAGCTCCTGT17200.13192146684399367No Hit
TCCCAGGGCTGTGCAGCCCCATGCTACCCCAGCACCCATCTCACAAGAGC17070.13092438598994022No Hit
CCCTGGAGAGGCTCCAGAGAGCGGAGTAAGACTGCAGCCCCTTCCCCTGT17010.13046419482653093No Hit
TCTCCAGGGTTTGGATGTGATGAGGGAGCTGCGCAGCACTTCAAGGAAAG17000.13038749629929605No Hit
TCCCTGAACCTGAGCCTCCAGGTGGGGAACTGCCCCCCACTGTAAGGGCA16950.13000400366312168No Hit
GCTTCAGACATCGCAACCTGCGTATTCTCAGCAGCAGTCTCAGCCACCCC16860.12931371691800775No Hit
GGGTTTGGGAGCAGCTTTGGATAGTTATGTCAAAACTGAACCATGTGTGT16810.12893022428183334No Hit
TTCCTCATGTGCCTTCACACATCTTCCAGGAGAGCTGTTCCATGATCTTC16790.12877682722736358No Hit
GGAGAAGAAAACAGGAGTTTTCTTCACTTTTTGTCACAGATGGGGCTGTC16590.12724285668266597No Hit
ACCTTATGGACAGCAGGGTCCCAGTGCATATGGGCAGCAAAGCCAGACTC16480.1263991728830823No Hit
GTATTGGGAACAGAGGGGAATGGTCACAGAAACCTCTGAGGTTTTATTTA16460.12624577582861254No Hit
TTTCTGCACAGGGGAAGGGGCTGCAGTCTTACTCCGCTCTCTGGAGCCTC16420.12593898171967302No Hit
GCATATGGGCAGCAAAGCCAGACTCCATATTACAACCAGCAAAGCCCTCA16380.1256321876107335No Hit
GACTGTGACTGCTGCTGTGAGTAGGGAGGCTGATTCTGTTGGTGCTGCTG16350.12540209202902886No Hit
AACCTGTACTAACACGTGGCTCTTTTTCTGCACAGGGGAAGGGGCTGCAG16280.1248652023383847No Hit
GGATTGGTATTGGGAACAGAGGGGAATGGTCACAGAAACCTCTGAGGTTT16280.1248652023383847No Hit
CTTATCAGCAGCAGCAACAGCCTCAGTCTCAAGCTCCACAGCTTCAGACA16090.12340793032092198No Hit
GACCACGGGTTAGCAGCCTGAGGGAAGCACAGGCCCTTTGGCTGGATGCT16030.1229477391575127No Hit
CCCAAACCCAGTGGATTTATAATAAACTCTGATGATAAGGTCTCCAGCCA15970.12248754799410343No Hit
GGTATGAAGATGACTGCTGAGAAAGAGCCGAGGAAGCTGTCTGCTGAGTT15940.12225745241239878No Hit
TCCCTATGCTGAATGTCCAGCATCCCACAGCTCTGTGTCCTTGGTTCATC15790.12110697450387557No Hit
GGAGTGCACTGACCACGGGTTAGCAGCCTGAGGGAAGCACAGGCCCTTTG15670.12018659217705702No Hit
GGTTATGTCAGATCACCTGCTCGTTCCTCATGTGCCTTCACACATCTTCC15540.11918951132300357No Hit
AGCAGGGAATATGCAGGCTGCTGCTGCTGCGGCTGTGACTGAGGGTATGA15480.1187293201595943No Hit
ATCCAGATGGCTGCATCTTGAGTCAGGTCCCTTTGGTCAAAAGCCTTATC15460.11857592310512453No Hit
TCTCACAAGAGCTTCCCTGGCACGAGGACGCTGACCTCGCTCCTCTGTAT15410.11819243046895013No Hit
GTCTTTGCTGAAGCGATGCTCCCAGGGCTGTGCAGCCCCATGCTACCCCA15290.11727204814213157No Hit
AAAGTATGTTTATTGTTAAAAACCTCCAAGCTGCCCTTGCTGCTGCCCCA15220.11673515845148741No Hit
GTCTCAAGCTCCACAGCTTCAGACATCGCAACCTGCGTATTCTCAGCAGC15140.11612157023360838No Hit
CCTCCAAGCTGCCCTTGCTGCTGCCCCAACATGGCTTGAGACTCTCCTAA15120.11596817317913863No Hit
AGCGAAAGCAGGACGCAAAGTACAATGGGCAGCATCTCGTATGCTCAGCA15060.11550798201572933No Hit
AAAGCAGGGAATATGCAGGCTGCTGCTGCTGCGGCTGTGACTGAGGGTAT15050.11543128348849446No Hit
GTGTATTCATTCCTCAACATCGTGGTGATATAAGAAAAACCTCTTGCCCT15020.11520118790678982No Hit
CAGCAGACACCATCCCAGACCCCTCACTCTCAGCCTTCTTATCAGCAGCA14980.1148943937978503No Hit
GGCATGATCTTCCCTTGGTGAAGCTGTGCTGGTTATGTCAGATCACCTGC14840.11382061441656197No Hit
AGCGAAAGCAGGGTGATGAATCACACTGGAGAAGAAAACAGGAGTTTTCT14720.11290023208974341No Hit
CATATGGGCAGCAAAGCCAGACTCCATATTACAACCAGCAAAGCCCTCAT14700.11274683503527366No Hit
CTCCAAGCTCTGCCAAGCATTTGGGATTGATAAGGCTTTTGACCAAAGGG14600.11197984976292485No Hit
GTGCAGCCCCATGCTACCCCAGCACCCATCTCACAAGAGCTTCCCTGGCA14530.11144296007228069No Hit
CCTTACCAGAGCCTGGATCCATTCACAAGGGAGAAAGTGGCTTTCAGTGC14260.10937209983693894No Hit
CTACAGAACAGACAGGTCAGAAAGATCCTCTGCTAAATGAAAAAGGAGAA14190.10883521014629477No Hit
CTCCAGTGCTGTCACTCTATCATAAAATGGCATCAGATTGGTCAGGCATG14180.10875851161905989No Hit
ATGTTGAGGAATGAATACACACATGGTTCAGTTTTGACATAACTATCCAA14140.10845171751012037No Hit
GAATCACACTGGAGAAGAAAACAGGAGTTTTCTTCACTTTTTGTCACAGA14110.10822162192841572No Hit
ACAATGGGCAGCATCTCGTATGCTCAGCAGATTCCACCTTATGGACAGCA14110.10822162192841572No Hit
GGGCAGCATCTCGTATGCTCAGCAGATTCCACCTTATGGACAGCAGGGTC14050.10776143076500644No Hit
TTCTAGTACTGTAGCCACTTATAGAGGCAGAGGATTGCTTGTTCATTTTC14040.10768473223777159No Hit
GTGATGAATCACACTGGAGAAGAAAACAGGAGTTTTCTTCACTTTTTGTC13840.10615076169307397No Hit
GATGATAAGGTCTCCAGCCAGCAAATGAGAGGAGAAAGAAGTCCAGATGC13680.10492358525731589No Hit
GCCTTTAACATGGTCCCCTATATTCTTATCTCTAAATTGGAGAGAGAGGG13590.10423329851220198No Hit
CTTCTGATGGTACCCAGGATATCATTGGCCTTCCAGTCTGCAGGTGCACA13530.10377310734879269No Hit
GGCTGCTGCTGCTGCGGCTGTGACTGAGGGTATGAAGATGACTGCTGAGA13520.10369640882155781No Hit
GAATTTGGACAAGTCCCTGGGGCTGGATGTCATGCAAGCCAGGGTCCTAA13500.10354301176708806No Hit
CTCCATATTACAACCAGCAAAGCCCTCATCCCCAACAGCAGCAGCCTCCA13490.10346631323985317No Hit
GATAAGGCTTTTGACCAAAGGGACCTGACTCAAGATGCAGCCATCTGGAT13480.10338961471261829No Hit
TCTTGAGTCAGGTCCCTTTGGTCAAAAGCCTTATCAATCCCAAATGCTTG13430.1030061220764439No Hit
GCCAGGGGCAGGGAATGCAACTGAAGTGCTTCTGCTGAGATGTTCCTTCT13410.10285272502197412No Hit
GCTGAGAAAGAGCCGAGGAAGCTGTCTGCTGAGTTTGCTGCTGCTGCTGC13390.10269932796750436No Hit
ATTCACAAGGGAGAAAGTGGCTTTCAGTGCTCAAATCCAACCTCAGTTCT13370.10254593091303461No Hit
GCTTGAGACTCTCCTAAAGAGCACAGCTCAAGTCCTGGTTGGTGGCACAC13260.10170224711345092No Hit
CCCTTCCCCTGTGCAGAAAAAGAGCCACGTGTTAGTACAGGTTTGTTCAG13240.10154885005898118No Hit
CTGTGGGTGCATCTCAAGATGTGAACCTTCAGGAAATTTGTTGATGACAC13210.10131875447727653No Hit
CTTGCAAGATTCATCAGCTGCAAGAGATGGGTTCCATTCCTGCCATCTCT13160.10093526184110212No Hit
GGATATGGAGTTGGGGACTGCTGATGGGGTGGCTGAGACTGCTGCTGAGA13090.10039837215045795No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)