FastQCFastQC Report
Mon 10 May 2021
HGJM2BGXJ_n02_ReSeq_1971_d02_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGJM2BGXJ_n02_ReSeq_1971_d02_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences505639
Sequences flagged as poor quality0
Sequence length151
%GC46

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATAGGGAACTTGGTTTGGTGAGATTAAATATTTTGTGCCTAGAAATCC14410.2849859286961646No Hit
ATATAGAACCCCCACTTTGACAGACCTTGATAGAGATGCCAGGCTTGATT14260.28201938537177706No Hit
CCATTAAGGGAGATCTAGTTGAACTTGCTCTCCAGAAAAAATATTTCCAA10900.21556881490549581No Hit
GTGTTAACAGACTGCTCAGGTGAACATGGGAGAAGTGTTTCAACACATAC10090.19954948095380298No Hit
TCCCTATAGAGGTCAAGCTCTTCTACCAAAACTTGTGTTCAGTTACTTTT8920.17641044302358005No Hit
CCCTTAATGGAGCCTGTGAACTCCCCAAAAACCAATAAAGGACTACAGCT8330.164742039280989No Hit
ATCTATAGCTGAAAGGTTTGAGACTCATTCTGTATGGCTATCGGTGATGT8010.15841341352229554No Hit
ATTAATTACAGCCATCCAGCTTGAGATCCTCCAGAAAAGCAGAGCTCACA7200.14239407957060274No Hit
CTCCAAAACAATCTAGAGACGGAACTGGGATTTTAATGTACTTCATCTAT7070.13982307535613353No Hit
GTTCTAAGTGATCCAAAGCAAGTTGCTACAAGGAAACAAAACAATTCCTA6830.13507660603711344No Hit
GCATATGGGTATGACTAAGGAGCAGAGATGCTCTTCGATCTTTACATCTC6790.13428552781727676No Hit
CTATAAAAGCAGTAAGTAGTTCACACCTGTCACTATCAGTGATGACACTG6630.13112121493793003No Hit
GTATTTTGTCAGCTTTTTTGTTTGTTTTAAACCGTTCTGCAATGCAAGCC6620.13092344538297085No Hit
CTTAAGGGCTGGTAGGTATTTGGAGCTGGAAGCTGCTTCCTTCACTTGAG6580.13013236716313417No Hit
CAATTAGCACATAGCCCTATTAATTACAGCCATCCAGCTTGAGATCCTCC6100.12063942852509398No Hit
ATATAGAAGGGTTCCTGCATACCAAAAATATGCCTGAAAGGACAACCATT6090.12044165897013481No Hit
CCATTGTGGAGCTGCTCTCTCTCTTTTTTAGCAGGGAACCCTGAGCGTAC6060.11984835030525731No Hit
GAACTGGGATTTTAATGTACTTCATCTATAGCTGAAAGGTTTGAGACTCA5660.1119375681068905No Hit
CCCATATGCAAATCATTTCCTAGAACGGTTTGGTGTACAAGGTGAGCTGT5630.11134425944201297No Hit
CCTTTGGAGTATGCCTTGTTCTTTGGTGATGGAATGTGTGATTTTTTTCT5590.1105531812221763No Hit
GTGTTTCAACACATACTCATTTATCGGAATTTTGTGGAAGTTCTAAGTGA5560.10995987255729878No Hit
ACATTAAGTAACGTCTTGTTGTTGGCTACATTTACATTACAGAGCTTGAA5540.10956433344738044No Hit
CTCTTATTCAAAAGTAACTGAACACAAGTTTTGGTAGAAGAGCTTGACCT5520.10916879433746211No Hit
GCCCTATTAATTACAGCCATCCAGCTTGAGATCCTCCAGAAAAGCAGAGC5210.10303793813372783No Hit
TGACTAAGGAGCAGAGATGCTCTTCGATCTTTACATCTCAAATAACATCA5190.10264239902380946No Hit
GTCCCAATTCAGTAAGGGATTGTTGTCAAAATATAGAAGGGTTCCTGCAT5090.10066470347421777No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)