FastQCFastQC Report
Mon 10 May 2021
HGJM2BGXJ_n02_ReSeq_1970_d10_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGJM2BGXJ_n02_ReSeq_1970_d10_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences136
Sequences flagged as poor quality0
Sequence length151
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCATAGGTCAGACATTGTGTTAAAAAAAAGTATCTATAAAGACGTGGAT21.4705882352941175No Hit
GCATAATTGTGTTTTGAAATGTGAGGACATGAGATTTGCGAGGGGCCATG21.4705882352941175No Hit
GGATATGCTCATGGGAGAAGAATTTATTGTGTTTATTTACAGAGGAAAAA10.7352941176470588No Hit
ATCATGACCTGAGCCGAAGGCAGACGCTTAACCGACCGAGCCACCAGGCG10.7352941176470588No Hit
TTACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCT10.7352941176470588No Hit
GGTTGATGGTGCCCCAGAGGGAGGCAGATGGCAAGAAACTGAGGGGGCCC10.7352941176470588No Hit
TTAATACCAAAGGACATTAGAGTCAAATACAATCCTGTTGTAAGAAATAA10.7352941176470588No Hit
ATCAAAAGCACATACAAAATTAATTCAAAAACATATCTTAAAAAACCAAA10.7352941176470588No Hit
ACTTAGAGCGTTTACAGATGAAGGAGCAATAGTGGGCGAAATCTCACCAT10.7352941176470588No Hit
GGCCTATGGGAGCCTTTCTCAGTCCACATTAAACTCAGAAACGCAGAAAC10.7352941176470588No Hit
CAAAGCATGTGACTTGAAAGTTAGATAGCGTAGTGAGAAATAAAATTGAA10.7352941176470588No Hit
CTCTACTAAAAATACAAAAAATTAGCCTGGCGTGGTGGTGGGCACCTGTA10.7352941176470588No Hit
CGAATGACCCGAATAGACATTAAATAAGAAATCAAAAACGAAAAACAAGC10.7352941176470588No Hit
ACTTGCTCTGTCGAGAGGTTCTGGGGCTGACCAAGTGCCCAGGAATCCAG10.7352941176470588No Hit
AACTAACGCAATATACCAAACATTCACATCACATTCTCGATAGGTAAAAA10.7352941176470588No Hit
ATGAAAGAGAACTGAGAACTGAAGCAGTCACACATAAATATGTCATTGAT10.7352941176470588No Hit
CCACAGAAGTGGCTCTTGGCCTAGTATGTGCCACTTGTGAACAGATTGCT10.7352941176470588No Hit
GCTCCAAGTGAATACTTGTGCACATATCTGTAAAAATTTTTTTACTGTTA10.7352941176470588No Hit
GGATAAGACCAAGAGAACTTATCACATATCTAAATAAAAACGTAGAATAC10.7352941176470588No Hit
GTACATGTGTGAAACTGGTCTGAATAACTTCTTTTGACATCTTCTGGAAA10.7352941176470588No Hit
TCACAATGGTAGGGAGAAGAGCAACAGCCATAATAAGAAAAGAAACCATG10.7352941176470588No Hit
GCTTGCGACTTTAGTTTAGAAACTGAGACATGGGTATCTGGGTATTCACT10.7352941176470588No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAAAGAATAAAAGAACTAA10.7352941176470588No Hit
ACCTTGCACTCTTTTTTTTTTTAACCTCTCTGAGCCATGCTTTCCACTGA10.7352941176470588No Hit
CCTACTTTGGGCATTCATTTTTTTTTCTGATACATTATCGTGCCCATTTT10.7352941176470588No Hit
ATAACACCCAGTTATGGTAACACCTCTGCCTCAGACATGTAGTAATACCA10.7352941176470588No Hit
TCACAGATACTTGGGAGGTCTCGATAAGGGCCGTGCTGGCCTCATAAAAT10.7352941176470588No Hit
GAGGTGGGAAGGGTAGTACTTAGGACACACGCCACCTTCAAGCACATTAA10.7352941176470588No Hit
TCATATAACTAATGAACTATTATCTTAAATTTTTGAAGATTGCCCGTAAG10.7352941176470588No Hit
TAATTTTATTGGATAAGCAAAGAGAATACTTGAAAGCTGAAATTCTACTG10.7352941176470588No Hit
CCTATGGCCCGGGCTGCTAGAGTGCCCGTGCTCTGCCTTCACCCAAGTGG10.7352941176470588No Hit
CTCCAACTTAGGTCATATGTAAGGCCACAAGACAAGTCAATAAATTCCAG10.7352941176470588No Hit
AATAGAACCATTTAACTATTAACAACCTAAATCCATTAGAGCCCAATACA10.7352941176470588No Hit
AGGGTCGGGACCCCCAGCCAAAGACCTAGCCTGCTCTGGTGAGGGTGAGC10.7352941176470588No Hit
GTATACCTGATCCCATCTCAACCCACAACCCCCAGCAGCATCCCCCATCA10.7352941176470588No Hit
GCTGCTTAACTGACTGTGCAACCCAGGTACCCCCAGAATAAGATTTTTTA10.7352941176470588No Hit
CGTGAGGAAGAATGAAACGGGTCCTGTTTTGCACCAGTCACTAAAAAGTC10.7352941176470588No Hit
CTCTAAATCCAGAATGATTTCAACTTGAGATCCTTGACTAATTACATCTG10.7352941176470588No Hit
ATTTGCTTGTTTCTTTGTTTGTGGCAAAGGCCAGTTTGAAAACATCGGAA10.7352941176470588No Hit
ATCTTTCCCTTAATGGTGATGATTAGACTTATTAGAGGCTCACATGCCAA10.7352941176470588No Hit
CAATAGGAGAACCAAAAATATGAAAGAACTGAATATAACTTCTAGGACTA10.7352941176470588No Hit
TTATTTTATAGCATTGTAGGGTTCTGGCAACACTGAATTTTAACAAATTC10.7352941176470588No Hit
TTTTTAAACTATTCGACACTAATTGATACCCATCCAAATACTATAGCACG10.7352941176470588No Hit
TCAGACCTCATTTGGGAAAATTCTAGGCTTCTGGAATTTATTGACTTGTC10.7352941176470588No Hit
CGGCAGGAGGTCCGGGAAGGGAAGGGAAGGGTGAGCGTTCGCCCCCTATT10.7352941176470588No Hit
GAACAATAACAGATCAACAAAGAGCTGGTTCTTTGAAAAGAACAAAGCTG10.7352941176470588No Hit
CTTAAACCCACTCGCCCTACAAATGGAAAACTCCGAAGACCTAGTTGCCC10.7352941176470588No Hit
GTGGTATAGAACTAGAAATCAATTACAAGAAGTAAACTGTAAATTCATAA10.7352941176470588No Hit
CCCTTGCCGTCCGCGCTCTGCCATGGTAGTAAAGCTCTTAAAGATCTCAT10.7352941176470588No Hit
CTTTTTCGTCATCCCAAACAGACGCTCTGTATTCATAAAAAAATAACTTC10.7352941176470588No Hit
GGTGAGCATTTTTATGTTTGTGTTCATCATATATAATTGGGATGTCTCGA10.7352941176470588No Hit
AAACTAAACTCAAGGACCCCAAGAGAGGAATGATCATGATGATAAAAGCA10.7352941176470588No Hit
AAAAAATTCATAAAATCCCCTCATATAGTATAAAACTGTTCACAGAATGT10.7352941176470588No Hit
CTCCCACACGTAAAACCAATATGAAAGTCTTATTTTTCCTAAACAACTAC10.7352941176470588No Hit
ATTGAAATTTTGTCTTTTGACATTTCAATTGGTAATTATAGAAAAAAAAT10.7352941176470588No Hit
GGGTCTTGAGCCGCGGCCCCCGGGGTCGGGCCCATCTCCGACGGCAGCTG10.7352941176470588No Hit
CTGTAACACCCAGCTCGGCTTTGTTCAGCCACCCCCTAGCAGGCACCTGA10.7352941176470588No Hit
CCCTTTTCCGTATCATAACCAACACAACGACTCCTTTCCCAATTAGCACA10.7352941176470588No Hit
GCAAAGCATCCTGCGCCAGCCAACTGCAAGCCCAAAAACGAAAGATTGCT10.7352941176470588No Hit
TGAACGAAGTTTTGATGTATAGATTTAGAGATAACATTAATAATTTTACA10.7352941176470588No Hit
TCTCTTGACCTTGTGATTCACCCGCCTCGGCCTCCCAAAGTGCTGGGATT10.7352941176470588No Hit
GAAATAAGCTACTTAGCTCCAAAGCAGATATTTTTCAACAAAACATAACG10.7352941176470588No Hit
GTCAATCACTGACCTCCTCGGGAGACAGTAGTACATAACCTCCTAGGTCC10.7352941176470588No Hit
TGGGTGCAGTTGGAGTCATCTCACGGCACATGGAATACACAATTTATCTT10.7352941176470588No Hit
GTATTCTGTTCCGTTGATCTATGTGTCTGTTTTTGGGCTTTGTACACTAT10.7352941176470588No Hit
GACCAAGAACTGGGTGATGCCCCATTCCTTGACCGGCTTCGCCGAGATCA10.7352941176470588No Hit
CTCTTGGGACTTGCTTTCCCGCGCTCCAACTACACGCCCGGGAGTCACCG10.7352941176470588No Hit
GTGCTGGAGTACTCCTCTACACCCATTTTGCTGAATCTCACTCCTATCAT10.7352941176470588No Hit
CTTAAAATGACTATCGCTTCAGTGCCTGCTTCACGCTACCTAACTGAAAT10.7352941176470588No Hit
GGCCATGGTATATTCACAATAGGATTGTATGATAAAAGCAGTCAGAGGTG10.7352941176470588No Hit
GTTTTGTATTTTCCTTTCTTCTTTCTTTCTTTCCTAGTCATTACTGTATT10.7352941176470588No Hit
AGGAATAAAGTTGTACCTCAAATTCTCATGGGAAATTGGGATTTAGAATG10.7352941176470588No Hit
GGGGAGGACAAAGAAAAAAGCATCTTTTGATCATTCTCCGGATAGCCTTC10.7352941176470588No Hit
AGCAGTCGAGTCCCCACTGAGCAAGGAGAGCAGTGCGAGATCTGCTCCCA10.7352941176470588No Hit
ATCATATTACACCATGTCACAGCCATAGGTGATACCATCACTCGGTAAGA10.7352941176470588No Hit
CTGCTTGGGAATGAGCATTAACCAATCCCTTGACATTTCCTCAAGAGTCA10.7352941176470588No Hit
TCCTTGAGTCAAATGCAACACTTCAATGTACACACTGCTTGTTCCACCAG10.7352941176470588No Hit
GCCCTTCGACAGTAGCGCAGGAGCAGGGTCCCGGGACCGGAACACGGAAT10.7352941176470588No Hit
CCCAAGCTCTGCGATAACGCTTGGCGATGTCCAATGAAGGGCAAAACTAC10.7352941176470588No Hit
CGTCGGCCGTGAAGCTCGACGACAGGTCGTACACGGCGTAGGCGACGACA10.7352941176470588No Hit
AACCGGAGCCGACTGCCCACATGCCTGTCCCGGAATGTCCAAAGTGCAGC10.7352941176470588No Hit
ATACTGCGCCTGCTAGGTGCTTCTAAATATCAAGAGGTAATATGAACATA10.7352941176470588No Hit
TTCGTGCTCGGTCCAGACTCGATGTCCATCCGTGGGTCAGTTCGGGCGGT10.7352941176470588No Hit
ACCTACTAAGATTGAATTCTAAAGAAACAGACATCTGACTGTTCAGCTGT10.7352941176470588No Hit
ACCTAATATTTTGACACCATGTTTAGCTAGTTTATCTGCTAAGTTAATTG10.7352941176470588No Hit
TTATCAAGGTGTCGTTCACTTTTATTGCTAAGAAGTATTCCATTATGTGG10.7352941176470588No Hit
CTTTAAAACAGATCCTTGGTTTGCGTATCAGGACTAGTCTAGAAACATCT10.7352941176470588No Hit
GGGGGGGCTACTCCACCCCGATTGTAGTGAGATTTCGGTTCCCCTTCAGC10.7352941176470588No Hit
GTTTGCTGGGTGGAAGTTAGTTAGAGGTATAGAATCCTTTTGTTTTCCAA10.7352941176470588No Hit
CCTTCGTTTTGTGAATGTGGCACATTAGTTTGATTTGTGGATGTTGAACC10.7352941176470588No Hit
CCTTGAACGTGTGCTACAAACTGAGAGAGATCAAAATAGGCTTCTTGCTG10.7352941176470588No Hit
TAGCGGCAATAATCAAGCTTTGATCGCCGTCATCATGCCGCACTTCCCCT10.7352941176470588No Hit
GTGTGTTTAGTCAGTATGTCTATCTATCTCTGTCCATCCATCCATCTTGA10.7352941176470588No Hit
GTAAGGAACAGGTATAGACAGAAGCAGGATACCTGCTTAGCATAGGGAAT10.7352941176470588No Hit
AACCAACATTCAAAAATATGTTCCAATAATTGGATTTGAATTACAAACAG10.7352941176470588No Hit
AGCTCAGTCCATGTGCTGTGGACAAGCGGACCCGCCCTTGGTCAGTGCAC10.7352941176470588No Hit
CTTTTAGTCTATTTATTGATTTATAGTTCTTTGGATGTAAGATTTGTTTA10.7352941176470588No Hit
CTGTGAGTACTACAGTAATGACGCAAGACAAAGTTGCGTTGCTGAAGGGC10.7352941176470588No Hit
CAGTTACTCAACTGGTGCGCTTGCCAGTTGCATGGGTCTCATATACAACC10.7352941176470588No Hit
AACCCACCTAGAGGAATGTTCTGGCCTTAGAAGTTGTTTAAAATAAAACC10.7352941176470588No Hit
TATCAATCCTTATCACCTCACATACACAGTAAATGAGGAGAACTGCATTC10.7352941176470588No Hit
TTATTGGCCTCTAATCGCCTTAGGTACTAAACACTGAAATGCTTAAAATT10.7352941176470588No Hit
GTCTGATACTCTCTGTGATTCTGGGGTCCTAAGGAAGACTTGGGGATCCA10.7352941176470588No Hit
CGGCTAAGGCCATGGAGCAGATGGCTGGATCAAGTGAGCAGGCAGCAGAA10.7352941176470588No Hit
AAAGAAAATAATTTTTAAATACCCATAAATGAATCCAAGCATCCAAAAGT10.7352941176470588No Hit
CTCAAGCAATCCTCCTGCCTTAGCCTCCTGAGTAGCTGGGACTACAAGCA10.7352941176470588No Hit
GGATTCGACTAATCTATTTAGTCCTCTAGCGGTCATCTGTCCATCTATTG10.7352941176470588No Hit
GCCAGTATTGAATCCTAAAGAAAAAGAAATATGAACAAACAAATGCGCAG10.7352941176470588No Hit
GCTATGCTGGCCTCGGGGTCAGAAGTGTAACTGGTGTGGTGGTAGTGATG10.7352941176470588No Hit
ATACTCACTACTAATAAATTCAAATAAAAATTAAATGCTCAAATAAAAAT10.7352941176470588No Hit
GCTCAGTCTTACAGGGCTCTCCTTGCCTCTGCGTGGCCCTTCTCACAGGG10.7352941176470588No Hit
GTAAATATGTTTTCTGTTGTATTAAGCAAAACAAATGATTTACTTTCAGG10.7352941176470588No Hit
AGTAGAAACAAGGTAGTTTTTTACTCCAGCTCTATGTTGACAAAATGACC10.7352941176470588No Hit
ACATATTTAGTTGCTCCATGCTGGGTGGGTAAATATTTATAAATGTTGCA10.7352941176470588No Hit
GTTTTGTGTTATTGTATTTTACCACAATTAGAAGTTTTAAAATTTAGTAA10.7352941176470588No Hit
GCACCCCATGGGGTCCGAAGTCCGTCAGCATCTGCACCGAGCCTGCAGAA10.7352941176470588No Hit
CTCTTAGACTCCGGCGCTCCGTCGGTAGGCGGGGCTCTTGTCGCACCGGA10.7352941176470588No Hit
GGCGAGCAGAGCTTATGTGCGAGAAAATGAATAGTTCAGGTTCCTGTTCA10.7352941176470588No Hit
CTGCTTGCTTGAGACCAGTCTCGAGGTGGGAGAAATCCACTACAGAGAAA10.7352941176470588No Hit
CCATCCAAGTAAGGGGTTGTCTGAGGCTGATGTTTCCATTGTGATCTTCT10.7352941176470588No Hit
GGGTGATACGGTGAGATGTGGTCTGGGGGGTGGGGCGGGGGAGTGAGAAG10.7352941176470588No Hit
ATATAAACAATTGTGTTCAAGCTCACTAACAACACTGACACAAGAATGAA10.7352941176470588No Hit
GGTTCAGGGACTTGCTCAGAGACAAGCCCACAGCCGGTGGGACAAAGTAT10.7352941176470588No Hit
AAGGTGAGCTGGGAGAGGGAAGACAATTTTGCTAAGAGGTGCTCATGAGT10.7352941176470588No Hit
GCACAGGGTGTGGGGACCAGCCAGGCGGCAGAAGAACAGGTCGGGGGGGC10.7352941176470588No Hit
CTCCAATACGAGGATGTGTATAAAAGTGCTTTATAAACTAGGAGATACTG10.7352941176470588No Hit
ACCCTCTGCCCATACATCCACAGAACAGTTTTCTTTCCCCTCTGATCTCA10.7352941176470588No Hit
CACCATCACCCAAGAGGAGGACGACTGGAGTCCAATTTCCGTTCTTTCCA10.7352941176470588No Hit
GATCCCAGTATACAGGGATCAAGTCATGAGCATAATGCAAACAATTAACC10.7352941176470588No Hit
ACTGCACTCCAGCCGGCAACAGAGTGAGAATCTATCACCAAGAAAAAAAG10.7352941176470588No Hit
GAATACAAGAATTGGTCAAAACCGCAATGTCAAATTACAGGGTTCGCCCC10.7352941176470588No Hit
CTTCTCGCCTTCCCCTGTTATGGCTTTAACGGAGTGGTGCTTTGGCATAG10.7352941176470588No Hit
ATCATAATCATCCATGAAACATACAGAGGTGATACAATCACAAGGTCTGA10.7352941176470588No Hit
CTCTGCATCCTGGGCGGTACAGGAGCTGCCAGCATACCTTTTCACGTCTC10.7352941176470588No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)