FastQCFastQC Report
Mon 10 May 2021
HGJM2BGXJ_n02_ReSeq_1970_d04_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGJM2BGXJ_n02_ReSeq_1970_d04_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences212209
Sequences flagged as poor quality0
Sequence length151
%GC48

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATAAAGACTATCCAATACTCAGTGTGGACAGATAATGATTATGTCCAG9740.4589814758092258No Hit
GTTATGGGCTTCCTTTGTACCTATGGGCTCAACCAGGTGGCCGTACTGGA8340.3930087790810003No Hit
GCCCATAACTGTGCACTTTTCTCCAAGCATCTCCCACCACTCGTTGCTGC8110.382170407475649No Hit
GTCTTTATTCTGTGCCCACATACCGCACAATTCAGCTTCTTCTTTGCCAA7230.34070185524647867No Hit
GTGTGGGAGCACAGCTCCCAGGGAGGCCAGTGGGGAACAAGAGCTAGGAG6990.3293922500930686No Hit
ATGTATAACCTTCCTTTAGTGGATGTCTCCTGTACAGTTATGGACCCATT6570.309600441074601No Hit
GATAATGATTATGTCCAGTGCTGTATCCCGCAGCCTGCAGCACACTCAGG6100.2874524643158396No Hit
GTACAAAGGAAGCCCATAACTGTGCACTTTTCTCCAAGCATCTCCCACCA5710.2690743559415482No Hit
ATCCAATACTCAGTGTGGACAGATAATGATTATGTCCAGTGCTGTATCCC5370.25305241530755057No Hit
GTGCTGTATCCCGCAGCCTGCAGCACACTCAGGTTATGGCCTGCAGGGTG5320.2506962475672568No Hit
ATTTACACCAGCTTTGCTGTGGCTGTGCATTCTGGTTGGCAAAGAAGAAG5210.24551267853861053No Hit
GAAGCAGACTGAGAGGTAGATGTTCACCTCCAAGTCCACTTGAGTCGCAG5020.2365592411254942No Hit
CTTTTGCACCAGTGAGAGTCACAGCTTCCCAACCTCTGCTCTCCCCAGCC4940.2327893727410242No Hit
GTATGTGGGCACAGAATAAAGACTATCCAATACTCAGTGTGGACAGATAA4820.22713457016431915No Hit
GCTGTATCCCGCAGCCTGCAGCACACTCAGGTTATGGCCTGCAGGGTGAG4670.22006606694343783No Hit
GTATTGGATAGTCTTTATTCTGTGCCCACATACCGCACAATTCAGCTTCT4560.21488249791479155No Hit
GTTATGGACCCATTTTTTGTCAGTGTGAGATCTCAGGGAGCTAAGAAAAG4330.20404412630944022No Hit
GTCCAGTGCTGTATCCCGCAGCCTGCAGCACACTCAGGTTATGGCCTGCA4310.2031016592133227No Hit
CTCCTGTACAGTTATGGACCCATTTTTTGTCAGTGTGAGATCTCAGGGAG4140.19509068889632392No Hit
CTCAGAAGCAGACTGAGAGGTAGATGTTCACCTCCAAGTCCACTTGAGTC4010.1889646527715601No Hit
CTCCAGTACGGCCACCTGGTTGAGCCCATAGGTACAAAGGAAGCCCATAA3920.18472355083903133No Hit
TTATAACATCTTCTGCCCACTTTTGCACCAGTGAGAGTCACAGCTTCCCA3900.18378108374291383No Hit
CCCACATACCGCACAATTCAGCTTCTTCTTTGCCAACCAGAATGCACAGC3880.18283861664679632No Hit
GGTTATGGCCTGCAGGGTGAGCAGGGGCCTGCAGAGCTGCTGCTCATTGC3870.18236738309873757No Hit
ATCTGGGGCCATAGGCATGGCGCTGGACTGGACCTGCCACATCTGTTGGC3780.1781262811662088No Hit
ATTATGTCCAGTGCTGTATCCCGCAGCCTGCAGCACACTCAGGTTATGGC3690.17388517923368002No Hit
ATCATTATCTGTCCACACTGAGTATTGGATAGTCTTTATTCTGTGCCCAC3670.1729427121375625No Hit
CCAATACTCAGTGTGGACAGATAATGATTATGTCCAGTGCTGTATCCCGC3660.17247147858950374No Hit
AAGCAGGAGAGATGAGTGGTGTGGGAGCACAGCTCCCAGGGAGGCCAGTG3580.16870161020503371No Hit
ATGCAGAACCCCTGCTCCTGTGGCTCCAAGAAGAGATCGAGAGGATCTGC3330.15692077150356487No Hit
GTGTAGGAAGAGCCTGAGGATGAGGCACGTATGTTTTGTCATCATGTGAG3330.15692077150356487No Hit
GTCAATGAGTGGCCATGGCCTCTCTTCCTCCCTGTTTTGCAATTTATAAC3280.1545646037632711No Hit
CCCTGTAACTGCACCAGAAGATAATTGCAGTAATGGCTCAATATGAAACT3260.15362213666715363No Hit
CCTTTGTACCTATGGGCTCAACCAGGTGGCCGTACTGGAGGGGCTGGCCA3180.14985226828268358No Hit
ATATGAAACTGCTCTGTGGAAGTTCAAACCAAAAGAAGCTGTGGGGGTAG3180.14985226828268358No Hit
GCTCCCAGGGAGGCCAGTGGGGAACAAGAGCTAGGAGGATTTGCTGCTGA3170.14938103473462483No Hit
ATTCTACTCTCTGCCCACAAGGACAGCATTGGGAATTCATAGTTAAGAAC3150.14843856763850732No Hit
ATACAGCACTGGACATAATCATTATCTGTCCACACTGAGTATTGGATAGT3070.1446686992540373No Hit
CCCCTGGGTCAATGAGTGGCCATGGCCTCTCTTCCTCCCTGTTTTGCAAT2940.1385426631292735No Hit
CTTCTTGGAGCCACAGGAGCAGGGGTTCTGCATATTCTTGAAAAAAGGCA2890.13618649538897973No Hit
GGACTGGACCTGCCACATCTGTTGGCAAAGTTGGGATGATGTCACCCACA2850.13430156119674472No Hit
CCTTATAACTGCCCTATGCCCTGGTTCAGGAGAACATTTGTATTGGGGTC2840.13383032764868596No Hit
CTCTTCCTCCCTGTTTTGCAATTTATAACATCTTCTGCCCACTTTTGCAC2810.1324166270045097No Hit
ATTTATAACATCTTCTGCCCACTTTTGCACCAGTGAGAGTCACAGCTTCC2780.13100292636033345No Hit
ATATAATTTTGAAGGAAAACAAAACCCCACAGTAACATCTTTTTCCTAGT2780.13100292636033345No Hit
GTCTAAAGCAGAATAAGCTGTAAGAACCAAAGATGTTTGCCCACATGATA2740.12911799216809844No Hit
CATGATACTTTGCCACATTTCCTTTGAAACACGTTCTTAAAAAACAGAAG2700.12723305797586343No Hit
GTACAGTTATGGACCCATTTTTTGTCAGTGTGAGATCTCAGGGAGCTAAG2660.1253481237836284No Hit
CCCTAGAACTTCCTGCAGAGGAAATCCTCTACCCTTCCATTGAATTTATC2620.1234631895913934No Hit
GCCTACCCCTGGGTCAATGAGTGGCCATGGCCTCTCTTCCTCCCTGTTTT2610.12299195604333463No Hit
GACTGAGAGGTAGATGTTCACCTCCAAGTCCACTTGAGTCGCAGTCTGGC2560.12063578830304086No Hit
GTCCACACTGAGTATTGGATAGTCTTTATTCTGTGCCCACATACCGCACA2560.12063578830304086No Hit
GGAGAGATGAGTGGTGTGGGAGCACAGCTCCCAGGGAGGCCAGTGGGGAA2560.12063578830304086No Hit
GTAGATGTTCACCTCCAAGTCCACTTGAGTCGCAGTCTGGCCAGCCCCTC2550.12016455475498211No Hit
GATGAGTGGTGTGGGAGCACAGCTCCCAGGGAGGCCAGTGGGGAACAAGA2520.11875085411080587No Hit
GGACCATACTGTTCAAGAATCATTTTTCCCTCTGAGAAAAGGAGCTTGGG2480.11686591991857083No Hit
GTGTGGACAGATAATGATTATGTCCAGTGCTGTATCCCGCAGCCTGCAGC2480.11686591991857083No Hit
ATTCAGCTTCTTCTTTGCCAACCAGAATGCACAGCCACAGCAAAGCTGGT2470.1163946863705121No Hit
GCCCTATGCCCTGGTTCAGGAGAACATTTGTATTGGGGTCGGACTTAAAC2460.11592345282245334No Hit
GTATTGGGGTCGGACTTAAACTTTTTGGAAGTTGCTAAATCCTGCTGGCT2440.11498098572633583No Hit
CCTCCAAGTCCACTTGAGTCGCAGTCTGGCCAGCCCCTCCAGTACGGCCA2420.11403851863021831No Hit
GCACTGGACATAATCATTATCTGTCCACACTGAGTATTGGATAGTCTTTA2420.11403851863021831No Hit
CCCCTGCTCCTGTGGCTCCAAGAAGAGATCGAGAGGATCTGCAGCAACGA2410.11356728508215957No Hit
GCTTTGAGGAGTAAGGTATGAAAATTTTGTGGGTATTTATTTTTGTTATC2390.11262481798604207No Hit
CCATAATCCTATCAATGTATAACCTTCCTTTAGTGGATGTCTCCTGTACA2350.11073988379380703No Hit
AAATTATATTTACTTATTTTTATTTTCTCTCAGAAGCAGACTGAGAGGTA2340.1102686502457483No Hit
GAGTATTGGATAGTCTTTATTCTGTGCCCACATACCGCACAATTCAGCTT2290.10791248250545452No Hit
GGTGTAAATAGGGGGTCATGTGGGTGACATCATCCCAACTTTGCCAACAG2280.10744124895739578No Hit
TTTGTATTGGGGTCGGACTTAAACTTTTTGGAAGTTGCTAAATCCTGCTG2270.10697001540933702No Hit
GTCTTAAATGTAGTTGATTAGGTATTCTGTTCTTTTTCTCATTGCTTGTT2270.10697001540933702No Hit
ATACCGCACAATTCAGCTTCTTCTTTGCCAACCAGAATGCACAGCCACAG2220.10461384766904326No Hit
GGTTGAGCCCATAGGTACAAAGGAAGCCCATAACTGTGCACTTTTCTCCA2190.10320014702486699No Hit
GAGCAGGGGTTCTGCATATTCTTGAAAAAAGGCAGCCCGGAGCTCGGGAG2190.10320014702486699No Hit
GCATATTCTTGAAAAAAGGCAGCCCGGAGCTCGGGAGGGAAGTAATGCTC2160.10178644638069075No Hit
GTACTGGAGGGGCTGGCCAGACTGCGACTCAAGTGGACTTGGAGGTGAAC2140.10084397928457323No Hit
ATCCCGCAGCCTGCAGCACACTCAGGTTATGGCCTGCAGGGTGAGCAGGG2140.10084397928457323No Hit
GTTCTGCATATTCTTGAAAAAAGGCAGCCCGGAGCTCGGGAGGGAAGTAA2130.10037274573651447No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)