FastQCFastQC Report
Mon 10 May 2021
HGJM2BGXJ_n02_ReSeq2_1794_d06_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGJM2BGXJ_n02_ReSeq2_1794_d06_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2297276
Sequences flagged as poor quality0
Sequence length151
%GC45

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCAACACTGTGTCAAGCTTTCAGGTAGACTGCTTTCTTTGGCATGTCC219330.9547394392315072No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTTAATCC144330.6282658243937602No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG131970.5744629726684995No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGATTCCAACACTGTGTCAAG104070.45301478794885763No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCT93930.40887555522279434No Hit
GTTGTAAGGCTTGCATAAAGGTTATTTGCTCAAAGCTATTCTCTGTAATT77700.33822666497190584No Hit
GAGCAAAAGCAGGTAGATGTTGAAAGATGAGTCTTCTAACCGAGGTCGAA75630.32921599320238404No Hit
GTGTTATCATTCCATTCAAGTCCTCCGATGAGGACCCCAATTGCATTTTT67180.29243329926399786No Hit
AAGCTATTCTCTGTAATTCTTAACCTATGTCGCATTTCTTCAATTAACCA62540.2722354649593693No Hit
GTCCTGGAAGGGAAGGTAATGGTGAGATTTCGCCCACTATTGCTCCTTCA57900.25203763065474066No Hit
GTGTCAAGCTTTCAGGTAGACTGCTTTCTTTGGCATGTCCGCAAACGATT55830.24302695888521886No Hit
CTATTGCTCCTTCATCTGTAAACGCTCTAAGTAGTATCAGAGCTTCAAGT55410.24119870664212748No Hit
GCATATAGTGGAGCGGATTCTGGAGGAAGAATCTGACGAGGCACTTAAAA54460.237063374187516No Hit
CTCTTGGTCTGGACATCAGAACTGCCACTCGTGAAGGAAAGCATATAGTG53230.23170920690417696No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCATTCTGCTGT52430.22822682167924097No Hit
GGTTAATGCTCATTCCCAAGCAGAAAGTGACAGGGCCCCTTTGCATTAGA48480.21103254463111965No Hit
GTATAGGTGGGTGGGTGATGTATGCCCCACACGAAAAGAATGCTCTTTCC48060.20920429238802826No Hit
GTACTAGCCAGCACTACGGCTAAGGCCATGGAGCAGATGGCTGGATCAAG44910.1954924005648429No Hit
GAGTGGAGATCTCCCATTCTCATCACTGCTTCTCCAAGTGAATCTCTGTA43080.18752644436280186No Hit
TTCTTAACCTATGTCGCATTTCTTCAATTAACCATCTTATCTCTTCAAAC41590.18104050188135862No Hit
CTCCTCAATTGCTCCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCA41530.18077932298948843No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAG41150.17912519000764382No Hit
AAGTAGTATCAGAGCTTCAAGTCGATTAAAAATCACACTAAAGTTTGCTT41130.17903813037702043No Hit
CTCATGGAATGGCTAAAGACAAGACCAATCCTGTCACCTCTGACTAAGGG40940.17821106388609814No Hit
GGCTAGTACCATTCTGTTCTCATGCCTGATTAGTGGGTTGGTGGTAGTCG39750.17303101586400588No Hit
ACCTAACTGAAATGACTCTTGAGGAAATGTCAAGGGATTGGTTAATGCTC39060.17002745860749863No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGATTCCAACACTGTGTCAAGCT37570.1635415161260554No Hit
GTATCAGAGCTTCAAGTCGATTAAAAATCACACTAAAGTTTGCTTTCAAT36550.15910147496426202No Hit
ACCCATTACTGCCTGGTCCATTCTAATGCAAAGGGGCCCTGTCACTTTCT36080.15705557364461215No Hit
AGAATGGACCAGGCAGTAATGGGTAAAACCATCATATTGAAAGCAAACTT35800.15583673881588456No Hit
ACTTAGAGCGTTTACAGATGAAGGAGCAATAGTGGGCGAAATCTCACCAT35330.15379083749623468No Hit
CTCATAGACTCAGGCACTCCTTCCGTAGAAGGCCCTCTTTTCAAACCGTA33900.14756607390666163No Hit
GTGCAGACCGTGCCAGAAAGATGAGATCTTCAATTTCAGCATTCCCAGGA33690.14665194778511595No Hit
CAACATGGACAGGGCAGTTAAACTATACAAGAAGCTGAAGAGGGAAATGA33370.14525899369514156No Hit
GTAATGGGTAAAACCATCATATTGAAAGCAAACTTTAGTGTGATTTTTAA33320.14504134461858303No Hit
TTCCAGGACATACTGACGAGGATGTCAAAAATGCAATTGGGGTCCTCATC33120.14417074831234905No Hit
CTCTTGCTCCACTTCAAGCAATAGTTGTAAGGCTTGCATAAAGGTTATTT33040.14382250978985547No Hit
ATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCTAAGTG32890.14316956256017999No Hit
GTCCTACATCGTCGAAAGATCATCAGCTGTAAATGGAACGTGTTACCCTG32570.1417766084702056No Hit
AGCCAGCACTACGGCTAAGGCCATGGAGCAGATGGCTGGATCAAGTGAGC30610.13324476466911245No Hit
GTGCTGGCTAGTACCATTCTGTTCTCATGCCTGATTAGTGGGTTGGTGGT30250.13167769131789125No Hit
GTCCATTCTAATGCAAAGGGGCCCTGTCACTTTCTGCTTGGGAATGAGCA30220.13154710187195617No Hit
CTCCCATTCTCATCACTGCTTCTCCAAGTGAATCTCTGTAGAGTTTCAGA30010.1306329757504105No Hit
GAACTAGGGTGAGTCCCAATTGTCCTCATAGCCTGCACCATTTGCCTAGC29900.13015414778198178No Hit
GCATTAACCAATCCCTTGACATTTCCTCAAGAGTCATTTCAGTTAGGTAG29880.1300670881513584No Hit
TTACTGCCTGGTCCATTCTAATGCAAAGGGGCCCTGTCACTTTCTGCTTG29600.1288482533226308No Hit
GAACTGGGTGATGCCCCATTCCTTGACCGGCTTCGCCGAGATCAGAAGTC29570.1287176638766957No Hit
GCATAAAGGTTATTTGCTCAAAGCTATTCTCTGTAATTCTTAACCTATGT29510.1284564849848255No Hit
CCCTAAGAGGAAGAGGCAGCACTCTTGGTCTGGACATCAGAACTGCCACT28970.12610587495799372No Hit
CACCTATACCGAGCAAACAAATTTGTACATAAGAAACGACACAACAACAA28120.12240584065649927No Hit
GCTTTAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATAT28100.12231878102587587No Hit
GTATAACAATAGAGAGAATGGTTCTCTCCGCATTTGATGAAAGGAGGAAC27990.12183995305744717No Hit
CTATCATCCCATCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGACTTGAG27810.12105641638183656No Hit
ATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAA27110.11800932931001759No Hit
TTCCTTGACCGGCTTCGCCGAGATCAGAAGTCCCTAAGAGGAAGAGGCAG27030.11766109078752401No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGTTCTTA26620.11587636835974431No Hit
CTGTAGAGTTTCAGAGACTCGAACTGTGTTATCATTCCATTCAAGTCCTC25910.11278575147261366No Hit
ATACTAGGCCAAGAGCCACTTCTGTGGTCACTGTTCCCATCCGGTTGTAT25750.11208927442762646No Hit
TTGTGCGACAGTGCTTTAATCCAATGATTGTCGAGCTTGCGGAAAAGACA25650.11165397627450947No Hit
CTCTCTATCATCCCATCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGACT25050.1090421873558075No Hit
TTCTAATGCAAAGGGGCCCTGTCACTTTCTGCTTGGGAATGAGCATTAAC24890.10834571031082028No Hit
CAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTG24830.1080845314189501No Hit
TACTAGGCCAAGAGCCACTTCTGTGGTCACTGTTCCCATCCGGTTGTATA24570.10695275622084591No Hit
GTTGGAAGAATGGTTGGTGGAATTGGGAGGTTTTACGTACAGATGTGCAC24530.1067786369595991No Hit
GTATATGAGACCCATGCAACTGGCAAGCGCACCAGTTGAGTAACTGAGTG24250.10555980213087153No Hit
TCTGTAAACGCTCTAAGTAGTATCAGAGCTTCAAGTCGATTAAAAATCAC24210.10538568286962471No Hit
CTGCTGTTCCTGCCGATACTCTTCCCTCATAGACTCAGGCACTCCTTCCG24190.10529862323900131No Hit
CCCTAGTTCCAGTGCAGGTCTAAAAGATGATCTTATTGAAAATTTGCAGG24020.10455861637870244No Hit
ATTCCAACACTGTGTCAAGCTTTCAGGTAGACTGCTTTCTTTGGCATGTC24010.10451508656339073No Hit
GAAGAACACAGATCTTGAGGCTCTCATGGAATGGCTAAAGACAAGACCAA23860.10386213933371523No Hit
GTTCTTATCTCTTGCTCCACTTCAAGCAATAGTTGTAAGGCTTGCATAAA23770.10347037099590994No Hit
CTCCAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCATTCT23720.10325272191935143No Hit
CTCATATACAACCGGATGGGAACAGTGACCACAGAAGTGGCTCTTGGCCT23710.10320919210403973No Hit
GTCTATATTCATCTCCACAACTCGAAGGATTTTCAGCTGAATCGAGAAAA23700.10316566228872803No Hit
ATCCAGAACAGTATAACAATAGAGAGAATGGTTCTCTCCGCATTTGATGA23660.10299154302748124No Hit
CTCATAGCCTGCACCATTTGCCTAGCCTGACTTGCGACTTCCATGGCTTC23530.10242565542842916No Hit
GCAAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTT23060.10037975410877926No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)