FastQCFastQC Report
Mon 10 May 2021
HGJM2BGXJ_n01_ReSeq_W17_Neg_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGJM2BGXJ_n01_ReSeq_W17_Neg_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences251399
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAGAACAGATCCATTAGAAAACAATTATACTCCAGTTTCTTCTGTAA39331.564445363744486No Hit
GTCCCTACACTGAGCAGCACTATTACTGTTATTGCTCCTGCTCATCATGG20560.8178234599182972No Hit
CTACTGGATCGCTTCCATGATCAAAAGGGCACATATCTCCTCTCATACAG18690.7434397113751448No Hit
GTGTAGGGACAGGATAGTGGCCAGATGAAATATTTGTTACAGAAGAAACT13430.5342105577190045No Hit
GAACAGATCCATTAGAAAACAATTATACTCCAGTTTCTTCTGTAACAAAT12970.5159129511255017No Hit
GTCCAGGAGGAGGTGGTCCTTCAACAACAGGGGGTTGTGCTGGAAAAGGC12100.4813066082203986No Hit
GTTCAGCACCGTGCATGGCAGCACTGCCAATCCAAATCCTAACCCAGCTC11570.4602245832322324No Hit
CTATTACTGTTATTGCTCCTGCTCATCATGGAAATAACACTACTGAAAGC11320.4502802318227201No Hit
ACTGAAAGCTGGTCAGAATTCCATGAAGAGCAGCTGGACCACAACTCCTA9980.3969785082677338No Hit
CTCATACAGAAACCCTTTTCATCATAATCTCTACAGCGTTTCTTTGGCAG9600.38186309412527497No Hit
GTGCTGAACACTGCAGTGTGGGGAAGTGGAGGTCCAGAACTCCCCAGAAA9240.36754322809557716No Hit
TTCAACAACAGGGGGTTGTGCTGGAAAAGGCAGAATGCCAGGAAGATTCA9150.36396326158815273No Hit
CTAAAATACGAAAGGGATTTGGGAAAGCCAAAATATGATATGGATAGAAC9060.36038329508072825No Hit
GTACTGGAGGTCTAAGGTTAACAGGAGGAGGACTCAGAATTGGTGGAGGG8750.3480522993329329No Hit
TCTCTACAGCGTTTCTTTGGCAGCGGAGGTCTTCCATAGGAGTTGTGGTC8190.3257769521756252No Hit
GTGCTGGAAAAGGCAGAATGCCAGGAAGATTCACATCTTCTACCACTACT7380.29355725360880514No Hit
GCTTTCAGTAGTGTTATTTCCATGATGAGCAGGAGCAATAACAGTAATAG7360.29276170549604413No Hit
GTTACAGGTGGAAGGCTAGGTGGTAAGGGGCCTGGTGGTGGTACTGGAGG7150.28440845031205375No Hit
CAATTATACTCCAGTTTCTTCTGTAACAAATATTTCATCTGGCCACTATC7060.28082848380462927No Hit
GATATGGATAGAACAGATCCATTAGAAAACAATTATACTCCAGTTTCTTC6700.2665086177749315No Hit
GTGGTAAGGGGCCTGGTGGTGGTACTGGAGGTCTAAGGTTAACAGGAGGA6630.26372419938026803No Hit
AAGTAGGAGCTGGAGCAAAGAGAGACTGCGGGACAGGGATCGAGACCGGA6510.2589509107037021No Hit
GAATTGGTGGAGGGGGAGGAAGTCCAGGAGGAGGTGGTCCTTCAACAACA6420.2553709441962776No Hit
GGTAAGTGGCATCCATTTATTGTTGAACTACTTGAAGGCCCGTGGTGGGG6280.2498021074069507No Hit
ACAGGGGGTTGTGCTGGAAAAGGCAGAATGCCAGGAAGATTCACATCTTC6200.24661991495590674No Hit
TTCTACCACTACTGGATCGCTTCCATGATCAAAAGGGCACATATCTCCTC6110.2430399484484823No Hit
GGTACCAACAGTTGGTCTCTAAAATACGAAAGGGATTTGGGAAAGCCAAA6010.23906220788467736No Hit
ATCCAGTAGTGGTAGAAGATGTGAATCTTCCTGGCATTCTGCCTTTTCCA5980.23786888571553588No Hit
ATCAAAATACCATCTATCCAAAATAAGCTTCATAAAATCAGGGGCCAGTA5880.23389114515173093No Hit
GTCCTGTTACAGGTGGAAGGCTAGGTGGTAAGGGGCCTGGTGGTGGTACT5810.23110672675706745No Hit
TATCTCCTCTCATACAGAAACCCTTTTCATCATAATCTCTACAGCGTTTC5490.2183779569528916No Hit
GTTCTATCCATATCATATTTTGGCTTTCCCAAATCCCTTTCGTATTTTAG5370.21360466827632568No Hit
GTACCAACAGTTGGTCTCTAAAATACGAAAGGGATTTGGGAAAGCCAAAA5350.21280912016356468No Hit
AAGCAGAGGTACCAACAGTTGGTCTCTAAAATACGAAAGGGATTTGGGAA5270.20962692771252073No Hit
CCATAGGAGTTGTGGTCCAGCTGCTCTTCATGGAATTCTGACCAGCTTTC5240.20843360554337925No Hit
AAGAGAGACTGCGGGACAGGGATCGAGACCGGAGCAGAAGCCGAAGCAGA5220.20763805743061825No Hit
AGCTACAGCAGCTCCATTAAAGATAGTTAATGGAAGTTATGATCTAAACG5200.20684250931785725No Hit
CTTCTAAGGTGTTTCTATTGTGCCAATCGAGAAAAGGGGAAAGATTTCAT5170.20564918714871577No Hit
CTGTAGAGATTATGATGAAAAGGGTTTCTGTATGAGAGGAGATATGTGCC5140.2044558649795743No Hit
GGATTTGGGAAAGCCAAAATATGATATGGATAGAACAGATCCATTAGAAA5080.20206922064129135No Hit
ACTTGAAGGCCCGTGGTGGGGGATGTAAAACATCCTTGTCTGGTTGGCAC4980.1980914800774864No Hit
TCTTCTACCACTACTGGATCGCTTCCATGATCAAAAGGGCACATATCTCC4970.1976937060211059No Hit
ATATGATATGGATAGAACAGATCCATTAGAAAACAATTATACTCCAGTTT4940.19650038385196442No Hit
CTGTATGAGAGGAGATATGTGCCCTTTTGATCATGGAAGCGATCCAGTAG4930.19610260979558392No Hit
GATCAAAAGGGCACATATCTCCTCTCATACAGAAACCCTTTTCATCATAA4890.1945115135700619No Hit
GGTCTAAGGTTAACAGGAGGAGGACTCAGAATTGGTGGAGGGGGAGGAAG4860.19331819140092044No Hit
GAATTAATATGTTCCCTTGTATTTCTCTAATGTTAGCTTAAGGGCTGGAT4780.19013599894987648No Hit
AATCTCTACAGCGTTTCTTTGGCAGCGGAGGTCTTCCATAGGAGTTGTGG4710.18735158055521303No Hit
CATATCTCCTCTCATACAGAAACCCTTTTCATCATAATCTCTACAGCGTT4690.18655603244245203No Hit
GTTAACAGGAGGAGGACTCAGAATTGGTGGAGGGGGAGGAAGTCCAGGAG4670.18576048432969106No Hit
GTATGAACATGGGGCTTTTTGTACCCAAACCCTCTTGGAGTGAGCTGGGC4650.18496493621693005No Hit
CAGGAGGAGGACTCAGAATTGGTGGAGGGGGAGGAAGTCCAGGAGGAGGT4540.18058942159674463No Hit
TCCTAACCCAGCTCCTCAAACCAGCGAGCTGGAAATGAAGCTCTCACACC4500.17899832537122262No Hit
GTCTAAGGTTAACAGGAGGAGGACTCAGAATTGGTGGAGGGGGAGGAAGT4450.17700945508932014No Hit
ATTATACTCCAGTTTCTTCTGTAACAAATATTTCATCTGGCCACTATCCT4400.1750205848074177No Hit
ATCCTGTCCCTACACTGAGCAGCACTATTACTGTTATTGCTCCTGCTCAT4380.1742250366946567No Hit
GAGCAGCACTATTACTGTTATTGCTCCTGCTCATCATGGAAATAACACTA4310.17144061829999324No Hit
GTTATGCAGAGGCAGGCATCATGCTTTAAAAGGTCGGAGCAAGTTTAAGC4290.17064507018723224No Hit
ATCATAATCTCTACAGCGTTTCTTTGGCAGCGGAGGTCTTCCATAGGAGT4280.17024729613085177No Hit
CTCCAAGAGGGTTTGGGTACAAAAAGCCCCATGTTCATACAGCAGCGACG4220.1678606517925688No Hit
ATTTTATTGTTTCCTTCTAAGGTGTTTCTATTGTGCCAATCGAGAAAAGG4030.16030294472133938No Hit
GGTTAACAGGAGGAGGACTCAGAATTGGTGGAGGGGGAGGAAGTCCAGGA4020.15990517066495888No Hit
CTACAGCGTTTCTTTGGCAGCGGAGGTCTTCCATAGGAGTTGTGGTCCAG3910.15552965604477345No Hit
CTCTCATACAGAAACCCTTTTCATCATAATCTCTACAGCGTTTCTTTGGC3890.15473410793201245No Hit
GAGCAGAAGCCGAAGCAGAACACGAAGCAGAGGTACCAACAGTTGGTCTC3860.15354078576287097No Hit
CCTTTAATAAAGTGTATTTAAAACTGGAGCTTTTCCCTCTGTGCTCACTC3850.15314301170649047No Hit
CTTCCATAGGAGTTGTGGTCCAGCTGCTCTTCATGGAATTCTGACCAGCT3830.1523474635937295No Hit
CCTGTGCACTGCTGCTGGAGAGAGAAGAAACTTTCACCTGCGGCAAAACA3820.151949689537349No Hit
GTTTGAGGGAGTGCTGGCTGGAAATGTTAATCAACATCTCCGACAAGGCA3820.151949689537349No Hit
CATAAAAGGCCTCTCAATCTTTGAGATAGGCCATTAAAATCCTCGAGCCC3730.14836972302992454No Hit
TGTTCATACAGCAGCGACGCTCCTGGTGACGGGTTTGCAGTGTTGCTGTA3680.14638085274802207No Hit
CCATTAAAGATAGTTAATGGAAGTTATGATCTAAACGTAAAATTGATTTT3680.14638085274802207No Hit
GTAGGAGCTGGAGCAAAGAGAGACTGCGGGACAGGGATCGAGACCGGAGC3650.14518753057888056No Hit
CTGCGGGACAGGGATCGAGACCGGAGCAGAAGCCGAAGCAGAACACGAAG3640.1447897565225001No Hit
TCTCTAATGTTAGCTTAAGGGCTGGATCATTGGGTAAGAAGTATTTTAAT3620.1439942084097391No Hit
GTATGAGAGGAGATATGTGCCCTTTTGATCATGGAAGCGATCCAGTAGTG3590.1428008862405976No Hit
GTAGAGATTATGATGAAAAGGGTTTCTGTATGAGAGGAGATATGTGCCCT3560.14160756407145614No Hit
TCCCTACACTGAGCAGCACTATTACTGTTATTGCTCCTGCTCATCATGGA3470.13802759756403168No Hit
TCCCCACACTGCAGTGTTCAGCACCGTGCATGGCAGCACTGCCAATCCAA3450.13723204945127068No Hit
GTGCATGGCAGCACTGCCAATCCAAATCCTAACCCAGCTCCTCAAACCAG3440.1368342753948902No Hit
GAGCAAAGAGAGACTGCGGGACAGGGATCGAGACCGGAGCAGAAGCCGAA3390.13484540511298773No Hit
CTTCTACCACTACTGGATCGCTTCCATGATCAAAAGGGCACATATCTCCT3390.13484540511298773No Hit
ATCTTATTCTGTACATTGCCTCTTTTCTTCTGCCTCCCCCATACACCTCT3380.13444763105660723No Hit
GTTATTGCTCCTGCTCATCATGGAAATAACACTACTGAAAGCTGGTCAGA3340.13285653483108525No Hit
GGATTTGGGGCTCCAGGCACTTGAGGGAGAAAACCACTCCGGACTGTGAA3340.13285653483108525No Hit
AGTTTCTTCTGTAACAAATATTTCATCTGGCCACTATCCTGTCCCTACAC3320.13206098671832428No Hit
TTAATAAAGTGTATTTAAAACTGGAGCTTTTCCCTCTGTGCTCACTCCAG3280.13046989049280228No Hit
GCTGTAGCTCCTGTAACTACCACATTTGGAGTTGAAGAGAAGGAGCATGA3200.12728769804175832No Hit
CTCTTGGAGTGAGCTGGGCTGGGGGGCGGTCCCCCAGGGGAAGCATGGAG3200.12728769804175832No Hit
GATCGAGACCGGAGCAGAAGCCGAAGCAGAACACGAAGCAGAGGTACCAA3150.12529882775985585No Hit
ACACTGAGCAGCACTATTACTGTTATTGCTCCTGCTCATCATGGAAATAA3140.12490105370347535No Hit
GGGATCGAGACCGGAGCAGAAGCCGAAGCAGAACACGAAGCAGAGGTACC3140.12490105370347535No Hit
CTCTACAGCGTTTCTTTGGCAGCGGAGGTCTTCCATAGGAGTTGTGGTCC3120.12410550559071436No Hit
AGCGAAAGCAGGAGTCGAAGTAGGAGCTGGAGCAAAGAGAGACTGCGGGA3060.12171886125243139No Hit
CAGTAATAGTGCTGCTCAGTGTAGGGACAGGATAGTGGCCAGATGAAATA3020.12012776502690942No Hit
GCCAAAATATGATATGGATAGAACAGATCCATTAGAAAACAATTATACTC2990.11893444285776794No Hit
CCACTATCCTGTCCCTACACTGAGCAGCACTATTACTGTTATTGCTCCTG2980.11853666880138744No Hit
TCATAATTCTGTCCTTGTCTGGGTTGATGTTTTTTGCCATTAACTTACCT2980.11853666880138744No Hit
CTCTTATCTCTTACCTTTTTTAACACAGCAAATAATTGTATTTTGTCCAA2970.11813889474500694No Hit
GTAAGGGGCCTGGTGGTGGTACTGGAGGTCTAAGGTTAACAGGAGGAGGA2970.11813889474500694No Hit
CTGCTAGATAGTGGTTGTGACAAGAAGATTGGTTTTGTTGGTAGTTCACT2940.11694557257586546No Hit
ACCAACAGTTGGTCTCTAAAATACGAAAGGGATTTGGGAAAGCCAAAATA2930.11654779851948496No Hit
GAGGTACCAACAGTTGGTCTCTAAAATACGAAAGGGATTTGGGAAAGCCA2920.11615002446310446No Hit
CAACAACAGGGGGTTGTGCTGGAAAAGGCAGAATGCCAGGAAGATTCACA2910.11575225040672397No Hit
GTCTTGTCCTGGTGCTATAAAACTCGGTGAACCTTAACTTTTAATATTTT2900.11535447635034347No Hit
GTGTTATTTCCATGATGAGCAGGAGCAATAACAGTAATAGTGCTGCTCAG2870.114161154181202No Hit
GTCCATAACAAATCTTACTTTGCATTTTTTTTCAGAAAGATGCCATGGCA2850.113365606068441No Hit
CTCCAGTTTCTTCTGTAACAAATATTTCATCTGGCCACTATCCTGTCCCT2850.113365606068441No Hit
ACTCCGGACTGTGAAGCTGACTGTAAGAGGGAAACATCTGAAAGCTCTGA2830.11257005795568002No Hit
CACTAAGGCCACTCGGCCATAAACAAGGACACCTTCCACAGAGTGCCATG2830.11257005795568002No Hit
CCTTAGAAGGAAACAATAAAATACATGCTTTTTAAAAGTAGCAAAGCAGG2820.11217228389929952No Hit
CTTTGAGATAGGCCATTAAAATCCTCGAGCCCGTTTGGTTCACAGAAGTC2810.11177450984291902No Hit
GAGCTGGAGCAAAGAGAGACTGCGGGACAGGGATCGAGACCGGAGCAGAA2780.11058118767377755No Hit
CCTCTCATACAGAAACCCTTTTCATCATAATCTCTACAGCGTTTCTTTGG2770.11018341361739704No Hit
ATCCAAAATAAGCTTCATAAAATCAGGGGCCAGTACAGCCTGGGATGGGA2760.10978563956101654No Hit
ACTCAGAATTGGTGGAGGGGGAGGAAGTCCAGGAGGAGGTGGTCCTTCAA2750.10938786550463607No Hit
GCACTATTACTGTTATTGCTCCTGCTCATCATGGAAATAACACTACTGAA2710.10779676927911408No Hit
GATTATGATGAAAAGGGTTTCTGTATGAGAGGAGATATGTGCCCTTTTGA2710.10779676927911408No Hit
TCCTTCAACAACAGGGGGTTGTGCTGGAAAAGGCAGAATGCCAGGAAGAT2690.10700122116635308No Hit
GTTATGATCTAAACGTAAAATTGATTTTTGCTCTTGAAGTAAAACTAAAG2690.10700122116635308No Hit
GTGTTTCTATTGTGCCAATCGAGAAAAGGGGAAAGATTTCATTTCATAAA2680.10660344710997259No Hit
CAACAGGGGGTTGTGCTGGAAAAGGCAGAATGCCAGGAAGATTCACATCT2670.1062056730535921No Hit
CCCCTGGGCTCCAAACAAAGGGAATAACAATTCACACGGGATTTGGGGCT2660.1058078989972116No Hit
TTATTAAGATGTGTTTTAGAGGCAGTTTGAGGGAGTGCTGGCTGGAAATG2650.1054101249408311No Hit
GTACAGCCTGGGATGGGATTGTGGCTTGACACATAAAAGGCCTCTCAATC2650.1054101249408311No Hit
TCATAATCTCTACAGCGTTTCTTTGGCAGCGGAGGTCTTCCATAGGAGTT2640.1050123508844506No Hit
GGTTTGCAGTGTTGCTGTAAAATACAGCTGGCCCCAATTGCAGCAGGGCC2610.10381902871530913No Hit
GTCTAAAACAAAAGGGGAAAAATAATGGCAGCTTCCATCCCGAAGCAGGA2590.10302348060254815No Hit
GTGCTCACTCCAGTTTAAAAGCAAATTATAGGCTACTTTCAAAATGTCTT2590.10302348060254815No Hit
GTCCTGGTGCTATAAAACTCGGTGAACCTTAACTTTTAATATTTTTCTTC2570.10222793248978715No Hit
CCCTTGTATTTCTCTAATGTTAGCTTAAGGGCTGGATCATTGGGTAAGAA2570.10222793248978715No Hit
GTTCACAGAAGTCTAAAACAAAAGGGGAAAAATAATGGCAGCTTCCATCC2570.10222793248978715No Hit
ACTTAAAAGGGTCCACAAGCTTCACCATGTCGGAACACGGATCCTACATA2560.10183015843340665No Hit
GCCATGCACGGTGCTGAACACTGCAGTGTGGGGAAGTGGAGGTCCAGAAC2560.10183015843340665No Hit
ATGTAAAACATCCTTGTCTGGTTGGCACTTCCTTGGCAGGTAAACCCAAA2560.10183015843340665No Hit
CCTCAAACCAGCGAGCTGGAAATGAAGCTCTCACACCTTCACCCCTTCTC2550.10143238437702617No Hit
GCATAGGAGCATCATTTGATCCCACAGAGCTCAGATCACTCTTTCTCCAC2540.10103461032064567No Hit
GGCCAGTACAGCCTGGGATGGGATTGTGGCTTGACACATAAAAGGCCTCT2540.10103461032064567No Hit
CTACTGAAAGCTGGTCAGAATTCCATGAAGAGCAGCTGGACCACAACTCC2540.10103461032064567No Hit
TATTTCATCTGGCCACTATCCTGTCCCTACACTGAGCAGCACTATTACTG2530.10063683626426517No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)