FastQCFastQC Report
Mon 10 May 2021
HGJM2BGXJ_n01_ReSeq_Sp19_Neg_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGJM2BGXJ_n01_ReSeq_Sp19_Neg_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24862
Sequences flagged as poor quality0
Sequence length151
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTATGGACTGCTTCATTGATTGCTATGGACTTTCTGCCTGTTAATCACT1210.48668650953262005No Hit
CCCTTGTACCCTGCAGAGTGACAGGAGGGAGGTAAGAGAGGCGATCAGAG1000.4022202558120827No Hit
GCTAATGGTCTGTACCAATTTATTCCACTTAGGCAGGGGCACTTCTCCCT950.38210924302147853No Hit
GTCTCAGATGGTGCTGAGCCTCCTCACCAGCAAGACCCGTCCCTCGCACC940.3780870404633577No Hit
GTGTAGAGCGTGTCGGCGTAGCGCTTGTTGGCCACATTGTCGACGTTGAG920.37004263534711607No Hit
ACCTACAGGATCAGCCCGCAATGGCGCTTGGGCGGCGGCTTGAACGCACG880.3539538251146328No Hit
GTCACAGCTCACAGAAATGGGCTGTGACTCCCAGCTCCCATTGGGCTTGG800.32177620464966616No Hit
TCCCAGTCCACTCCCACAACATAACATGACACAGAGATCACTCCTCAGCT760.30568739441718284No Hit
ATCTGAGACAGAGTGGTGACATTATGCTAGATGGAAAGCAAGGCTGTCAG690.27753197651033706No Hit
GTCCTGTTCAGTTCAGCCATGCAATGTCTGTGAGAGGCAAGAGTTACCTT610.24535435604537045No Hit
GTTCCAGCCCAGCGCCCAGCTGTCACTGCATCTGATGGGCGCGACCTCGT580.23328774837100796No Hit
CTTCAAGCCCTTTCTTCTCTAAGTGGCCCCAGTAGAGGGAGGGAGTCCCT580.23328774837100796No Hit
GTCCAGCAGTGCGCGGGCACGGGCGACGCCGGCGGCATCGAGGATCTGGG570.22926554581288713No Hit
GTACAAGGGTGGGCTCTGGGGGGCACAGGGCCATTGAAGTCCCCAAGCAG560.2252433432547663No Hit
ACATAACATGACACAGAGATCACTCCTCAGCTGGCAAAGCACTGCACAGC540.21719893813852467No Hit
CATCTAAGGAGCATGGCCTATTGGTTCAGAGCCCAGGTGGACTCAGCCTG530.21317673558040381No Hit
GTATCAGGAGATAAGATCTTATTCTGAGCCCTTCAAGCCCTTTCTTCTCT530.21317673558040381No Hit
GTCCACGGAGGAGGAAGGAGCTCTCCCCTGCGTTTCTTTGAGAGTGGAAC530.21317673558040381No Hit
GCTATGGACTTTCTGCCTGTTAATCACTATTTCTCATCCCAGTCCACTCC520.209154533022283No Hit
CACCCAGCCCTGTGCATAAAGGATCTCTGTCCTGTTCAGTTCAGCCATGC520.209154533022283No Hit
TTCCTGGGTCTTCTCTGCCTTTTCTTTGCAACAGAAAGAGAAGGGATTTA500.20111012790604135No Hit
CCTTAGATGTCACACAACTCCTCTTATTTAGCATCATGTCTTTCCACGTT490.19708792534792052No Hit
CTGCAGAGAGGCAGACCTGGGGAACTGATGGTCCACGGAGGAGGAAGGAG490.19708792534792052No Hit
ATTCAAAGCAGCTGTTCACCCACTAGAGTGGGCTCCCTTCAGCAACCTCC490.19708792534792052No Hit
GTCTGAGTCTGTGTGCTACAAGGCACGAAGGGTGCTGCATGAAACAGAGC480.1930657227897997No Hit
GTCTGTGAGAGGCAAGAGTTACCTTATTCCCACAGCCTGAACCGCCTTCT480.1930657227897997No Hit
TCTCAGAGCCTTCCCTGGCTTTTCCTCCTGTCGCAGGGAGAAGTGCCCCT480.1930657227897997No Hit
GGTCTACAGCGACAGCCCCGGACTGACGTTCGCGCAACGCAGCAACTTCT480.1930657227897997No Hit
TCTCTGTCCTGTTCAGTTCAGCCATGCAATGTCTGTGAGAGGCAAGAGTT470.18904352023167886No Hit
CGCCTATACCTCACGCTCGGCCGGCGCCTATCTGCAGGACATGATCTCGA460.18502131767355803No Hit
GCTCAGGGGCGACTACGACAGCTTCAACTACAGCTACAACGCGACCGGCA440.1769769125573164No Hit
CTCCTGTGGCAGTAAACTCACTGGGTGATGCCGTGAGTGAGGAGAGCAAC440.1769769125573164No Hit
GTACCAATTTATTCCACTTAGGCAGGGGCACTTCTCCCTGCGACAGGAGG430.17295470999919557No Hit
ACACAGAGATCACTCCTCAGCTGGCAAAGCACTGCACAGCCATCTCCTCT430.17295470999919557No Hit
GGATTTAGGAGGAGGGGGAACTGAAATCCCTTTCATTCGTGCCAGCAGTG430.17295470999919557No Hit
GTGTAGCGGTTGAACAGCGGCGGCAGCACGCGCTTGGGCAGCGTGGCCGA420.16893250744107474No Hit
GCCATCTCCTCTTCCTCAAAACAAACAAAAACAAAAAACAACAAAAAAAC420.16893250744107474No Hit
GACTCAGACTGATGGCTCCCAGGCTGAGTCCACCTGGGCTCTGAACCAAT420.16893250744107474No Hit
GCCTGGGAGCCATCAGTCTGAGTCTGTGTGCTACAAGGCACGAAGGGTGC420.16893250744107474No Hit
GTGTTGAGCGGGTCGGTGTTGCGCTCCTGCAGCTTGGTCGCGCGGAAGAT420.16893250744107474No Hit
GCTTCATTGATTGCTATGGACTTTCTGCCTGTTAATCACTATTTCTCATC410.1649103048829539No Hit
CTCCTCAGCTGGCAAAGCACTGCACAGCCATCTCCTCTTCCTCAAAACAA410.1649103048829539No Hit
TTGTAGCACACAGACTCAGACTGATGGCTCCCAGGCTGAGTCCACCTGGG400.16088810232483308No Hit
CATTAAGGAACGTGGAAAGACATGATGCTAAATAAGAGGAGTTGTGTGAC390.15686589976671225No Hit
CCACAGGAGAGGGGAGATGCTCTCCTGGCTCAGCAGCAAGAGGGAAGCTT390.15686589976671225No Hit
GTCGTACTGGCCGCCGAGCTCGACGTTGACCGATTGCTCCGGCGGGATGC390.15686589976671225No Hit
ACATGACACAGAGATCACTCCTCAGCTGGCAAAGCACTGCACAGCCATCT390.15686589976671225No Hit
GGCCTGGACGTCGACATCACCGGCCGCATCACCGACGCCTGGGAGGTCTA380.15284369720859142No Hit
ATCCAGAGCAGTGCCGACAGCTCCCTCCCTCCCCCCTGCCGGAGGAGGAC380.15284369720859142No Hit
ATGCTAGATGGAAAGCAAGGCTGTCAGGCTGAGCTCTGTTTCATGCAGCA380.15284369720859142No Hit
CACTTAGGCAGGGGCACTTCTCCCTGCGACAGGAGGAAAAGCCAGGGAAG380.15284369720859142No Hit
CTCTCATTCTTTCCTTCTCTTACCGCCACGCTGCAGAGAGGCAGACCTGG380.15284369720859142No Hit
CGCTTGGGCAGCGTGGCCGAGAAGAAGCCGGGATGGCCGTCCAGCGCCTG370.14882149465047062No Hit
GCTCTGAGATGGCCCCTTAAGGAATTGGTTGTTGCTCTCCTCACTCACGG370.14882149465047062No Hit
CTCCTGGCTCAGCAGCAAGAGGGAAGCTTGAGGCTGGAAGGGATGGGTGC370.14882149465047062No Hit
GTTTCATGCAGCACCCTTCGTGCCTTGTAGCACACAGACTCAGACTGATG370.14882149465047062No Hit
GCCGTAGACCTCCCAGGCGTCGGTGATGCGGCCGGTGATGTCGACGTCCA360.1447992920923498No Hit
TCCTGCAGGCGGTCAGAACTTGTAGGTGCCGGTCAGCGCGACGATGCGGC360.1447992920923498No Hit
GCTCTACACCGGCCATTACGTTCCGGGCAAGGGCCGCATCGTCGCGCTGA350.14077708953422896No Hit
GCCTGAACCGCCTTCTCTCTCCATCGTGGCTGGGAGGCTGCTCCCATTCA350.14077708953422896No Hit
CTATTTCTCATCCCAGTCCACTCCCACAACATAACATGACACAGAGATCA350.14077708953422896No Hit
GTCCAGGCCTGCGGCGTGGCGCTTGCCGGACAGGGTCACGACATTGGTGT350.14077708953422896No Hit
ACCGTGTACTCGGCCATCAGGTTGGCGATGATGAACTTGGGCGCATAAAA350.14077708953422896No Hit
CTCCCACACTCGCTCCCTCTCTCATTCTTTCCTTCTCTTACCGCCACGCT340.13675488697610813No Hit
TCTCTGCCTTTTCTTTGCAACAGAAAGAGAAGGGATTTAGGAGGAGGGGG340.13675488697610813No Hit
GTGCTGGAGATCACTTTGCCTTCCTTTCTCAGGACGTCACACTGCCTTGC340.13675488697610813No Hit
CCTTAATGTGAGGACAATTGCACCAAGCCCAATGGGAGCTGGGAGTCACA330.1327326844179873No Hit
GAGGAGGAAGGAGCTCTCCCCTGCGTTTCTTTGAGAGTGGAACGGAGAGG330.1327326844179873No Hit
GCACTGCACAGCCATCTCCTCTTCCTCAAAACAAACAAAAACAAAAAACA330.1327326844179873No Hit
CACACACACACACTTGGAAATCTCTTCTCTTTAAGCAGCCTCAAAGTTGG330.1327326844179873No Hit
CCTGTAGGTCGTCCACAGGCTGCCGGAGTAGCGCGGCGTCAGCGACGGCC330.1327326844179873No Hit
GCAGAGAGGCAGACCTGGGGAACTGATGGTCCACGGAGGAGGAAGGAGCT330.1327326844179873No Hit
CCTAAATCCCTTCTCTTTCTGTTGCAAAGAAAAGGCAGAGAAGACCCAGG330.1327326844179873No Hit
GTGCTGAGCCTCCTCACCAGCAAGACCCGTCCCTCGCACCCATCCCTTCC320.12871048185986647No Hit
GAGTGGGAGGGCGCAAGAAAGACTCCAAGCAATTTTTCTATCTCTTTCAG320.12871048185986647No Hit
CTCCCACTCCCTGCCCATTGCATGCACACATACACACACCCAGCCCTGTG320.12871048185986647No Hit
CTACAGGATCAGCCCGCAATGGCGCTTGGGCGGCGGCTTGAACGCACGCA320.12871048185986647No Hit
GGCCTGCAGCGCGCGGAACTGCTCGCGATCCTGTTCGGTGACGGGCTGCA310.12468827930174563No Hit
CACCCATCCCTTCCAGCCTCAAGCTTCCCTCTTGCTGCTGAGCCAGGAGA310.12468827930174563No Hit
TCTCTCTCCATCGTGGCTGGGAGGCTGCTCCCATTCATGCTTTAATCCCT310.12468827930174563No Hit
CTGCACAGCCATCTCCTCTTCCTCAAAACAAACAAAAACAAAAAACAACA310.12468827930174563No Hit
GTAGCACACAGACTCAGACTGATGGCTCCCAGGCTGAGTCCACCTGGGCT310.12468827930174563No Hit
CCCCTCTCCTGTGGCAGTAAACTCACTGGGTGATGCCGTGAGTGAGGAGA310.12468827930174563No Hit
CCTGCAGCTTGGTCGCGCGGAAGATGCCGAAGCGCGTCGTCAGCTTGCCG310.12468827930174563No Hit
GTATTTAAAGGGTGCAGAAGGGGTCTTGGGGGCTGCAGTGCTGGAGATCA310.12468827930174563No Hit
CTGCTGGACACCGCACCCTGGGCCGATGGCCGCAGCAGCGCCGGCACCCA300.1206660767436248No Hit
CATCAGATGCAGTGACAGCTGGGCGCTGGGCTGGAACAGCACGGCGGCGC300.1206660767436248No Hit
CTTTAATCCCTGGGAACAGAGGCACGATTCAAAGCAGCTGTTCACCCACT300.1206660767436248No Hit
CTCGACGACCGTGTACTCGGCCATCAGGTTGGCGATGATGAACTTGGGCG300.1206660767436248No Hit
TGCCAGCAGTGGTGAGAGCCAACTTTGAGGCTGCTTAAAGAGAAGAGATT300.1206660767436248No Hit
GCCATGCAATGTCTGTGAGAGGCAAGAGTTACCTTATTCCCACAGCCTGA290.11664387418550398No Hit
GCCCAACACCACGGTCGGCAGCCCGGACGACGGCGCCAGCATCGACGAGT290.11664387418550398No Hit
GTGCAATTGTCCTCACATTAAGGAACGTGGAAAGACATGATGCTAAATAA290.11664387418550398No Hit
CTTCTACGCGACGCTCGGGCTCACCAAGCCCAACACCACGGTCGGCAGCC290.11664387418550398No Hit
TTTTTAATCCAGGCAGCAACCAAAACGCTCCTTCATCATCTCTCTGCCTC290.11664387418550398No Hit
GGAGAGGGGAGATGCTCTCCTGGCTCAGCAGCAAGAGGGAAGCTTGAGGC280.11262167162738315No Hit
TATTCCCACAGCCTGAACCGCCTTCTCTCTCCATCGTGGCTGGGAGGCTG280.11262167162738315No Hit
GTACGACAGCGCCGACGGCAAGCTGACGACGCGCTTCGGCATCTTCCGCG280.11262167162738315No Hit
GAACGGAGAGGCAGAGAGATGATGAAGGAGCGTTTTGGTTGCTGCCTGGA280.11262167162738315No Hit
ATCTTATTCTGAGCCCTTCAAGCCCTTTCTTCTCTAAGTGGCCCCAGTAG280.11262167162738315No Hit
CCTATTCGCTGAGCCCCGCCAATGCCAGCATCCCGCCGGAGCAATCGGTC280.11262167162738315No Hit
CCCACAGCCTGAACCGCCTTCTCTCTCCATCGTGGCTGGGAGGCTGCTCC280.11262167162738315No Hit
AAACAGAGCTCAGCCTGACAGCCTTGCTTTCCATCTAGCATAATGTCACC280.11262167162738315No Hit
ATCCTGGAGAGCCACACACAAGCCCACGAGGGGAGCCCCAGAGCAGGTGC280.11262167162738315No Hit
GTGTTGAACGAGGTCGCGCCCATCAGATGCAGTGACAGCTGGGCGCTGGG280.11262167162738315No Hit
TCTCTAAGTGGCCCCAGTAGAGGGAGGGAGTCCCTAAGGTCCCACTGCAC280.11262167162738315No Hit
GCTTAAAGAGAAGAGATTTCCAAGTGTGTGTGTGTGTGTGTGTTGTTCTT270.10859946906926234No Hit
CTTTAAGCAGCCTCAAAGTTGGCTCTCACCACTGCTGGCACGAATGAAAG270.10859946906926234No Hit
ACCTTATTCCCACAGCCTGAACCGCCTTCTCTCTCCATCGTGGCTGGGAG270.10859946906926234No Hit
GACTACGACAGCTTCAACTACAGCTACAACGCGACCGGCACGCCGGGCTA270.10859946906926234No Hit
GTGTTGCGCTCCTGCAGCTTGGTCGCGCGGAAGATGCCGAAGCGCGTCGT270.10859946906926234No Hit
CCCCTGCGTTTCTTTGAGAGTGGAACGGAGAGGCAGAGAGATGATGAAGG270.10859946906926234No Hit
AGGTAATTCACCTCCAACTTCAGGGCTGGATGTTGGCTCGTGATAAGCTG270.10859946906926234No Hit
GTGCAGAAGGGGTCTTGGGGGCTGCAGTGCTGGAGATCACTTTGCCTTCC260.1045772665111415No Hit
CTCCCACAACATAACATGACACAGAGATCACTCCTCAGCTGGCAAAGCAC260.1045772665111415No Hit
GGCTGGAACAGCACGGCGGCGCGCTTGCTCCACGCCGAGACGTTCATCCG260.1045772665111415No Hit
CACATACACACACCCAGCCCTGTGCATAAAGGATCTCTGTCCTGTTCAGT260.1045772665111415No Hit
ATGCTAAATAAGAGGAGTTGTGTGACATCTAAGGAGCATGGCCTATTGGT260.1045772665111415No Hit
GTGTCCAGCAGTGCGCGGGCACGGGCGACGCCGGCGGCATCGAGGATCTG260.1045772665111415No Hit
GTGCTACAAGGCACGAAGGGTGCTGCATGAAACAGAGCTCAGCCTGACAG260.1045772665111415No Hit
GCCTTGCCTGGGGAAGGCTGGCTGGGAAAAAAATGCCCATGTCTCGCCTC260.1045772665111415No Hit
CTCCTCACTCACGGCATCACCCAGTGAGTTTACTGCCACAGGAGAGGGGA250.10055506395302068No Hit
AGCGAAAGCAGGTGGGCAGGCAGCAGCGGCAGGGCAGCAAGAGGTAATTC250.10055506395302068No Hit
GCTACAACGCGACCGGCACGCCGGGCTACGACAAGATCAGCGCCACGACG250.10055506395302068No Hit
GCCATCAGGTTGGCGATGATGAACTTGGGCGCATAAAAGCCGGGGTTGCG250.10055506395302068No Hit
GTCGTCGAGGACGCGCTGCTGTTCCGACTCAACGTCGACAATGTGGCCAA250.10055506395302068No Hit
CCCCAGTAGAGGGAGGGAGTCCCTAAGGTCCCACTGCACTGTCACAGCTC250.10055506395302068No Hit
TACACACACCCAGCCCTGTGCATAAAGGATCTCTGTCCTGTTCAGTTCAG250.10055506395302068No Hit
GCCTAGCTGTGAGCGTGGCATTCTGAGGCATATGTACTGTGGTGTTCAGC250.10055506395302068No Hit
GGAAAAGGGAAAGCATCATTTATGGACTGCTTCATTGATTGCTATGGACT250.10055506395302068No Hit
TCTCATCCCAGTCCACTCCCACAACATAACATGACACAGAGATCACTCCT250.10055506395302068No Hit
GTTCAGTTCAGCCATGCAATGTCTGTGAGAGGCAAGAGTTACCTTATTCC250.10055506395302068No Hit
CCTGGGAACAGAGGCACGATTCAAAGCAGCTGTTCACCCACTAGAGTGGG250.10055506395302068No Hit
GTGTGCTACAAGGCACGAAGGGTGCTGCATGAAACAGAGCTCAGCCTGAC250.10055506395302068No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)