FastQCFastQC Report
Mon 10 May 2021
HGJM2BGXJ_n01_ReSeq_2870_d06_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGJM2BGXJ_n01_ReSeq_2870_d06_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2520806
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACG126590.5021806517439263No Hit
AATCTAATCTGTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACATCT104840.4158987244555908No Hit
GGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGA97350.3861860055871019No Hit
GGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGAT74390.295104026251921No Hit
TCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGG67560.26800951759080227No Hit
GGAGCAAACGCGCCAGCGTCGCATCCGGCATTTATTCCGCACCAACGCAA65610.26027389652357225No Hit
CATTAGTACCGGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGCCTA63240.25087214168801564No Hit
GATTTAATCTGTATCAGGCTGAAAATCTTCTCTCAATCCGCCAAAACAGC59800.23722571272838927No Hit
GTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGC57650.22869669462862274No Hit
GTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACATCTTCGGCGTTGT55520.2202470162321099No Hit
GTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTT52650.20886176881521226No Hit
GTTTCATTAACCCGCTTGTCGCCGGTTAATCTAATCTGTATCAGGCTGAA51680.20501379320741064No Hit
GTTTTACGATGTCCGATAATTTATAACATTTCGTACGGTTGGAAAAATGT50370.1998170426442971No Hit
CGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTG49330.19569137807510772No Hit
GAGCAAAAGCAGGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATC48460.1922401009835743No Hit
AATCTAATCTGTATCAGGCTGAAAATCTTCTCTCAATCCGCCAAAACAGC47780.18954255107295048No Hit
GTCTTCCGACTGGGCCTTTCGCTTAATTTGATGCCTGGCAGTTCCCTACT46980.1863689629428048No Hit
GAGTGGACGCATCACTGGTGTTCGGGTTGTCATGCCAATGGCACTGCCCG45770.18156891089595947No Hit
GACATGAGTTGTCCGATATTCGATTTAAGGTACATTTTTATGCGACTTTT44740.17748291617839693No Hit
TTGTAAGGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCAACGCATC44680.177244897068636No Hit
GTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGA44110.1749837155259072No Hit
TAGTACGAGAGGACCGGAGTGGACGCATCACTGGTGTTCGGGTTGTCATG43390.17212748620877608No Hit
GTGTAAGCGCAGCGATGCGTTGAGCTAACCGGTACTAATGAACCGTGAGG42930.1703026730339423No Hit
AACATCTTCGGCGTTGTAAGGTTAAGCCTCACGGTTCATTAGTACCGGTT41980.16653403712939432No Hit
TTCTAGGATCCTCCGGCGTTCAGCCTGTGCCACAGCCGACAGGATGGTGA41910.16625634816800658No Hit
TCCTGGGGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGT41410.16427285558666554No Hit
GTTTGGCACCTCGATGTCGGCTCATCACATCCTGGGGCTGAAGTAGGTCC40100.159076105023552No Hit
ATACTGAGCTGATGAGCAATTTCCGTTGCACCAGTGCCCTTCTGATGAAG39010.15475209119622851No Hit
GGGTCAGGTGGGACCACCGCGCTAAGGCCGCCAGGCAAATTCTGTTTCAT38630.1532446368344093No Hit
CATCACTGGTGTTCGGGTTGTCATGCCAATGGCACTGCCCGGTAGCTAAA38560.15296694787302156No Hit
GGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCG38550.15292727802139475No Hit
ATCTAATCTGTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACATCTT37460.14860326419407127No Hit
GGTCTCAACCAGTCAGCAGTCTCTTGATCTTCAGGTCAGAGCACTCAAAG36720.14566769517368652No Hit
TCACTGGTGTTCGGGTTGTCATGCCAATGGCACTGCCCGGTAGCTAAATG36250.1438032121472259No Hit
GTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCTAGTA35490.14078830342358753No Hit
GTCATGCCAATGGCACTGCCCGGTAGCTAAATGCGGAAGAGATAAGTGCT35460.14066929386870708No Hit
TCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGT35200.13963787772640973No Hit
ATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGT35180.13955853802315607No Hit
ACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATG34930.13856679173248557No Hit
GATAAGCAAGAGTAGGCTGGAGCAAACGCGCCAGCGTCGCATCCGGCATT34900.1384477821776051No Hit
GAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAG34890.13840811232597827No Hit
CGCTAAGGCCGCCAGGCAAATTCTGTTTCATTAACCCGCTTGTCGCCGGT34700.1376543851450687No Hit
CAATGGCACTGCCCGGTAGCTAAATGCGGAAGAGATAAGTGCTGAAAGCA34480.13678164840927862No Hit
GATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATA32780.13003777363271907No Hit
GGATAAGACGCGCCAGCGTCGCATCCGGTATTAATTGATATGCAAAAAAC32120.12741956342534888No Hit
GTGCTGAAAGCATCTAAGCACGAAACTTGCCCCGAGATGAGTTCTCCCTG31880.12646748698630517No Hit
ACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTG31870.12642781713467835No Hit
GGAGTGGACGCATCACTGGTGTTCGGGTTGTCATGCCAATGGCACTGCCC31470.12484102306960551No Hit
GTCCAGGGCCAGGCAAATTCACGATACTGATAGTGCTGACGTATCAGCGC31400.12456333410821777No Hit
TATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCTAGTACGAGA31050.12317488930127904No Hit
GTACGAGAGGACCGGAGTGGACGCATCACTGGTGTTCGGGTTGTCATGCC31010.12301620989477176No Hit
TCGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCA30640.12154842538457937No Hit
GGATAAGACGCGCCAGCGTCGCATCCGGCATTTTTTTCTGCGCCAATGCA30550.12119139671993798No Hit
GTACTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGATGTTTTGGC30020.11908889458371649No Hit
GAGCAAAAGCAGGACGGATAAGCAAGAGTAGGCTGGAGCAAACGCGCCAG29300.11623266526658536No Hit
GATTTAATCTGTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACATCT29160.11567728734380987No Hit
CCTAGTACGAGAGGACCGGAGTGGACGCATCACTGGTGTTCGGGTTGTCA29000.11504256971778075No Hit
GCCCGGTAGCTAAATGCGGAAGAGATAAGTGCTGAAAGCATCTAAGCACG28800.11424917268524433No Hit
GAGTAGGCTGGAGCAAACGCGCCAGCGTCGCATCCGGCATTTATTCCGCA26970.10698958983753608No Hit
GGTGGGACCACCGCGCTAAGGCCGCCAGGCAAATTCTGTTTCATTAACCC26960.10694991998590926No Hit
GTTCGGTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCC26460.10496642740456823No Hit
TTGATAGGCCGGGTGTGTAAGCGCAGCGATGCGTTGAGCTAACCGGTACT26140.10369699215250995No Hit
CTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGATGTTTTGGCGGA25810.10238788704882486No Hit
AATCTGTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACATCTTCGGC25640.10171349957116889No Hit
GATTAGATTAACCGGCGACAAGCGGGTTAATGAAACAGAATTTGCCTGGC25590.1015151503130348No Hit
GTACCGGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGCCTATCAAC25550.10135647090652752No Hit
GGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGCCTATCAACGTCGT25340.10052340402236429No Hit
CATCACATCCTGGGGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCAT25320.10044406431911064No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)