FastQCFastQC Report
Mon 10 May 2021
HGJM2BGXJ_n01_ReSeq_2251_d04_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGJM2BGXJ_n01_ReSeq_2251_d04_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1484285
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAGTGCAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAA63680.4290281179153599No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG61210.4123871089447107No Hit
GCATGGAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAAG54010.3638789046577982No Hit
GTAGCATGTTTTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG47990.323320656073463No Hit
GTTTTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTGGAATTGA41410.27898954715570123No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA40050.2698268863459511No Hit
ACCCCTTACTTGGATGGACTTGACAGATACACACAGAACCCTCCATCCAA38650.26039473551238473No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC32780.22084707451736021No Hit
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT28920.19484128721909877No Hit
CTTATATGGACTTGACAGATACACACAGAACCCTCCATCCAAAAGCAGCA27420.18473541132599197No Hit
GTTTTCTTCTTGTAATTGATTTCTAGTTCTATACCACTGTGGTTGGAAAA25570.17227149772449363No Hit
GGATCATTCCTTCCTCTTGTTTTTCTGGCAGAGTTGGAGAAGGATTGGTA25500.1717998901828153No Hit
TTACTGCCCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA24460.1647931495635946No Hit
CCCAACTGGTTGTTCAGTAGCATGTTTTTTAGTCTCTGTGTTTATGAATT23130.1558326062717066No Hit
GTTGACATGGTCCTATGATGTTGATCCTTCGTTTTGTGAATGCGGCCCAT21690.14613096541432408No Hit
GCAGTGCAGCTACTAAATATGTAGAGCATATATGAACAAACCTAAGAAAA21430.14437928025951888No Hit
ATTTCCAGTTTTCTTCTTGTAATTGATTTCTAGTTCTATACCACTGTGGT20950.14114539997372472No Hit
CTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTCATT20010.13481238441404447No Hit
AAGTTGGAGAACGTTCTAGGGGCACTTGAGAGGAGTATATTCCTTTTGCT19480.13124164159848006No Hit
AGTCTGTTCAGATGTCTTTTTCTTTAGGATTCAATCTTAGTAGGTTGTAT18780.1265255661816969No Hit
GTTTTCTTCTTGGAATTGATTTCTAGTTCTATACCACTGTGGTTGGAAAA18070.12174211825895971No Hit
ACCTGTTACTTAGATGGACTTGACAGATACACACAGAACCCTCCATCCAA17510.11796925792553316No Hit
GCTCTTAAGAATGTACTATGAACAATTACACCCCAACAAGCTGGATAATC17380.11709341534813057No Hit
GTATTGGGAGTCAGGATATGGTCATTGGAGAAGAATTTATTGTGTTTATT16890.11379216255638237No Hit
GTTTACTTCTTGTAATTGATTTCTAGTTCTATACCACTGTGGTTGGAAAA16720.11264682995516359No Hit
ACCCCTTACTTGGATGGACTTGACAGATACACACAGAACTCTCCATCCAA16430.11069302728249628No Hit
CATCACCCTCAGACAACCCCTTACTTGGATGGACTTGACAGATACACACA16270.10961506718723156No Hit
GCATGGAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAAAAG15700.10577483434785098No Hit
GTTGACATGGTCCTATGATGTTGATCCTTCGTTTTGTGAATGCGGCACAT15690.10570746184189693No Hit
TGTTTTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTGGAATTG15570.1048989917704484No Hit
CTTGTAATTGATTTCTAGTTCTATACCACTGTGGTTGGAAAAGATGGTTG15210.10247358155610277No Hit
CTTCCCTCAATGATCCCCCATCCAGACAGAAGATCACAAAGGAAACATCA15080.10159773897870018No Hit
GTCTTTTTCTTTAGGATTCAATCTTAGTAGGTTGTATGTTTCTGGAAATG15050.10139562146083804No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)