FastQCFastQC Report
Mon 10 May 2021
HGJM2BGXJ_n01_ReSeq_2235_d06_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGJM2BGXJ_n01_ReSeq_2235_d06_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCCTGGGTCTTGGTCGCCTTGTCAGCTGGCCTTGGGCCACTGAGGCCAA17.6923076923076925No Hit
GAAGAAGCTAGACAAGGAAGGGAGATGAGGAGGAGCGAACCGTGCGAAGG17.6923076923076925No Hit
GATCATATGTGAAGTAAAATAAGCTAGTTGTCAAACGACAAGTACTATAT17.6923076923076925No Hit
GTAGAGAGATTAAATAAGAAATCAAAAACTTCCAACAAGCAAAAGTCCCC17.6923076923076925No Hit
GGTCGGATGTGTTCCTTCTACTAACTTTGGCCTTTATTTGTTCTTTTCTG17.6923076923076925No Hit
CCCCGCCGAGCAGAGAGCCCGATGCAGGACTCAGTCCCAAGACCCTGGGA17.6923076923076925No Hit
AGACAGACAAGCAATTAACTATAATGAGATAATTGCTCTATTAGAGGTTT17.6923076923076925No Hit
GGTGGGGCTTGAGACTCTGCATTTCTCACAGGATTACAAATGCTGGCCAC17.6923076923076925No Hit
GTGGATGAAGAACCAAAGTATAAAATTGCAACAAAACAAAACAAAAACAA17.6923076923076925No Hit
CAATACAACCCCTATATATAAACAAGATTTAGTTTCCCTTGATCTCTGAA17.6923076923076925No Hit
GCTGGCACAAAAACAGACACATAGATCAATGGAACAGAATAGAGCGCCCA17.6923076923076925No Hit
GACCATAAAAAAGAAGTACAAATCACCAATACAGGAGAACAACCAAGCAC17.6923076923076925No Hit
GCCATTAGCCGCCACCATCCGGTAGGTCCGTGGGCCCAGGAAATGATATC17.6923076923076925No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)