FastQCFastQC Report
Mon 10 May 2021
HGJM2BGXJ_n01_ReSeq_2235_d04_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGJM2BGXJ_n01_ReSeq_2235_d04_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1441100
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG34770.24127402678509471No Hit
GCAGTGCAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAA24640.17098050100617582No Hit
GCATGGAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAAG24270.16841301783359933No Hit
GTAGCATGTTTTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG21790.1512039414336271No Hit
ACCCCTTACTTGGATGGACTTGACAGATACACACAGAACCCTCCATCCAA20720.14377905766428423No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA19530.13552147664978142No Hit
GTTTTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTGGAATTGA19340.13420303934494482No Hit
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT17750.12316980084657553No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC16390.11373256540142945No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)