FastQCFastQC Report
Mon 10 May 2021
HGJM2BGXJ_n01_ReSeq_1984_d10_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGJM2BGXJ_n01_ReSeq_1984_d10_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2026870
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAGCGGCAGCAACGCCAAAGCCAGCATTGAACTGAACATGCAGGCGT53990.2663713015634945No Hit
CTCCCACACCAGGCCATGGAGATCCCTCACGCCGTAAGCATTGGGAGCGC52990.2614375860316646No Hit
CTCCATGCCGGATCGTCTCGGGCGTCGGGTCGGGTGTCGTCTGCCGCCGC49210.2427881413213477No Hit
CATCAGGATGGCGTAGTTGTCGCGTTGCTGCAGGTTGATGGCACCGGCGC47710.2353875680236029No Hit
GAATACAACTCGGTCATGCCGCCGATGAACCAGCTCAACGACGACGAGGT47590.2347955221597833No Hit
GGTCAAGGTCGATCGCTTCAGCATGCGAACCACGCCTGTCACCCGCGTCG42600.21017628165595229No Hit
GGCCTGGGCCGCGAACCAACTGACATGGGTGACCGGCTGCTGCGATTGAG38790.1913788254796805No Hit
CCTATGTCCTCAACGCCTGGGACAACGCTGGCGGGCGCGTCACGACCGAC36820.18165940588197566No Hit
CTCCCATTCGTTCCATGTCGGCAGGCGCGCGCTTTCAGACTCGCAGAAGG36780.18146205726070247No Hit
GTGCCGGCCAGTCTGCGAGGTAGCTGGCGTCGGCGAACACGCGCGAGGCT36700.18106736001815607No Hit
TCCCCATACTGTGTCCAGCTCTCTTTGTATGTTCACAGAGGTGGAGAGAC32640.1610364749589268No Hit
CTTTCAGACTCGCAGAAGGCCTGGGCCGCGAACCAACTGACATGGGTGAC32480.16024708047383404No Hit
ACCTTGACCAAGCCGCCCGTGGCGTCGCCCGCGAGGACGCTGCGCATCGT32160.15866829150364847No Hit
GTCTGCGAGGTAGCTGGCGTCGGCGAACACGCGCGAGGCTCTGCCGCGCT31450.15516535347604926No Hit
ATCCTCACCTATGTCCTCAACGCCTGGGACAACGCTGGCGGGCGCGTCAC30030.14815947742085087No Hit
GTGCGGGCGGTGCCGGCCAGTCTGCGAGGTAGCTGGCGTCGGCGAACACG25920.12788190658503013No Hit
GTGCTGCACGGCCTCTCAGGCAAGGTCAAGGTCAACGGCCAGGAATACAA25320.1249216772659322No Hit
AGCGAAAGCAGGGCGACGCGCATCAGGATGGCGTAGTTGTCGCGTTGCTG24840.12255349381065388No Hit
CACCTATGTCCTCAACGCCTGGGACAACGCTGGCGGGCGCGTCACGACCG24680.12176409932556109No Hit
ATTTTAGAGTGATCGCTTACACTGAAAAATATTTTGGCCTAAGTTTCATA22230.10967649627257792No Hit
GACATGGGTGACCGGCTGCTGCGATTGAGACGAAGGCAGTGCGGGCGGTG22020.10864041601089365No Hit
GGCCGGCACCGCCCGCACTGCCTTCGTCTCAATCGCAGCAGCCGGTCACC21090.10405206056629188No Hit
GCCTGGGACAACGCTGGCGGGCGCGTCACGACCGACGACGTGCGAAAGGT20320.10025309960678286No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)