FastQCFastQC Report
Mon 10 May 2021
HGJM2BGXJ_n01_ReSeq_1971_d04_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGJM2BGXJ_n01_ReSeq_1971_d04_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1303806
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATATTACAACCAGCAAAGCCCTCATCCCCAACAGCAGCAGCCTCCATA66070.5067471694408524No Hit
GGGAATATGCAGGCTGCTGCTGCTGCGGCTGTGACTGAGGGTATGAAGAT61590.47238622923962614No Hit
CTCCTAAAGAGCACAGCTCAAGTCCTGGTTGGTGGCACACTTGCAAGATT54600.418773958702445No Hit
GGAATATGCAGGCTGCTGCTGCTGCGGCTGTGACTGAGGGTATGAAGATG53560.41079731187001745No Hit
GTACTAACACGTGGCTCTTTTTCTGCACAGGGGAAGGGGCTGCAGTCTTA47530.36454809994738485No Hit
GAATATGCAGGCTGCTGCTGCTGCGGCTGTGACTGAGGGTATGAAGATGA43760.33563275517983504No Hit
GAGTAGGGAGGCTGATTCTGTTGGTGCTGCTGGGCTGTGGACTGCTGCTG41930.321596924695852No Hit
CTCGTATGCTCAGCAGATTCCACCTTATGGACAGCAGGGTCCCAGTGCAT41670.3196027629877451No Hit
GTCCCAGTGCATATGGGCAGCAAAGCCAGACTCCATATTACAACCAGCAA38950.29874076357985774No Hit
ATTCTAGCCTGCCCCATGAATAAAATTCCACCTTGCTGCAATAAAGTATG38730.29705339598069036No Hit
ATCCCAGACCCCTCACTCTCAGCCTTCTTATCAGCAGCAGCAACAGCCTC38120.29237478581936266No Hit
CTGTAAGGGCAGAGCAAGTCCCAGACCAACTCATGAGACTGAATTTGGAC38010.29153110201977905No Hit
ATGCAGGCTGCTGCTGCTGCGGCTGTGACTGAGGGTATGAAGATGACTGC37940.29099421232913486No Hit
GTGCATATGGGCAGCAAAGCCAGACTCCATATTACAACCAGCAAAGCCCT36870.2827874699150027No Hit
GACTGAGGGTATGAAGATGACTGCTGAGAAAGAGCCGAGGAAGCTGTCTG35930.27557780835492396No Hit
TGTTAGTACAGGTTTGTTCAGAAGGAACATCTCAGCAGAAGCACTTCAGT35040.26875163943101965No Hit
ATCCAAAGCTGCTCCCAAACCCAGTGGATTTATAATAAACTCTGATGATA34430.26407302926969195No Hit
GTCCAGGTACCATCCAGATGGCTGCATCTTGAGTCAGGTCCCTTTGGTCA33720.2586274338360155No Hit
GTATGAAGATGACTGCTGAGAAAGAGCCGAGGAAGCTGTCTGCTGAGTTT32930.25256825018446No Hit
GTCTTACTCCGCTCTCTGGAGCCTCTCCAGGGTTTGGATGTGATGAGGGA32500.24927021351336012No Hit
GAATATAGGGGACCATGTTAAAGGCCTTGCAGAAGTTCAGGTGGATGACA31260.239759596136235No Hit
GTTCTCCATCATATTTGAAAAGTCGTGGCTGTCAGGCAAAGTTCCTGGGG28990.22234903045391724No Hit
GGGTATGAAGATGACTGCTGAGAAAGAGCCGAGGAAGCTGTCTGCTGAGT28490.21851410409217323No Hit
GTCCTAAAGGAGCTGGCTGATGTGGTTGCTGAGCTGTTCTCCATCATATT28270.21682673649300585No Hit
TCCATACTCTCAGCAGACACCATCCCAGACCCCTCACTCTCAGCCTTCTT28140.21582965563895243No Hit
GTCTACAACACTCACAAATTTGTTTCCCCGTAAATAAAACCTCAGAGGTT27490.2108442513686852No Hit
GCTTTGGATAGTTATGTCAAAACTGAACCATGTGTGTATTCATTCCTCAA27480.21076755284145035No Hit
GTTAAAGGCCTTGCAGAAGTTCAGGTGGATGACATCAGATGCCTTCCCTT27460.21061415578698056No Hit
CTTATGGACAGCAGGGTCCCAGTGCATATGGGCAGCAAAGCCAGACTCCA27010.20716272206141098No Hit
ATCATAAAATGGCATCAGATTGGTCAGGCATGATCTTCCCTTGGTGAAGC26770.20532195740777387No Hit
GTGTAATGGGCCCTCCAAGCTCTGCCAAGCATTTGGGATTGATAAGGCTT26730.20501516329883432No Hit
CCTATATTCTTATCTCTAAATTGGAGAGAGAGGGATTTGAAAGATGGACT26370.20225401631837867No Hit
GCTGATAGGGTGACTGTGACTGCTGCTGTGAGTAGGGAGGCTGATTCTGT25780.19772880321152075No Hit
GAGTAAGACTGCAGCCCCTTCCCCTGTGCAGAAAAAGAGCCACGTGTTAG25360.1945074650676558No Hit
ATAATAAACTCTGATGATAAGGTCTCCAGCCAGCAAATGAGAGGAGAAAG25310.19412397243148136No Hit
GTACCTGGACAGGAGCTGCCAGGGGAGCAGGACGTGGTAGCCACGACCAG25280.19389387684977671No Hit
GAAGATGACTGCTGAGAAAGAGCCGAGGAAGCTGTCTGCTGAGTTTGCTG25190.19320359010466281No Hit
CTCTCAGCCTTCTTATCAGCAGCAGCAACAGCCTCAGTCTCAAGCTCCAC25080.19235990630507913No Hit
CTGCTGGCTCATGTTAAGTTTTTGATCAACCAGAACCTTAAGTCCTTCTC24850.1905958401786769No Hit
GATATGGAGAAGGCTGAGGTACTCAATGAGTTCTTAGCCTGAGGCTGGCT24440.1874512005620468No Hit
ATATTACAACCAGCAAAGCCCTCATCCCCAACAGCAGCAGCCTCCATACT24030.18430656094541673No Hit
GGGGAAGGGGCTGCAGTCTTACTCCGCTCTCTGGAGCCTCTCCAGGGTTT23860.18300268598242375No Hit
TCCCTGAACCTCACATCCCTGAACCTGAGCCTCCAGGTGGGGAACTGCCC23840.18284928892795402No Hit
CCTTATGGACAGCAGGGTCCCAGTGCATATGGGCAGCAAAGCCAGACTCC23720.18192890660113542No Hit
CTCTGGAGCCTCTCCAGGGTTTGGATGTGATGAGGGAGCTGCGCAGCACT23430.17970464931132393No Hit
GTATCACCCAAGGCCGTGTTTGTCACACCCACACCTCAGCCTGTCACTTG23070.17694350233086825No Hit
GCATAGGGAAGGGAGTGCACTGACCACGGGTTAGCAGCCTGAGGGAAGCA22730.17433575240488233No Hit
GCAATAAAGTATGTTTATTGTTAAAAACCTCCAAGCTGCCCTTGCTGCTG22580.17318527449635912No Hit
TTCCTCAACATCGTGGTGATATAAGAAAAACCTCTTGCCCTCTGGCAAGC22490.1724949877512452No Hit
CCTCAACATCGTGGTGATATAAGAAAAACCTCTTGCCCTCTGGCAAGCCT22310.17111441426101737No Hit
ATTACAACCAGCAAAGCCCTCATCCCCAACAGCAGCAGCCTCCATACTCT22220.17042412751590344No Hit
TCCCAATACCAATCCTGGTCGTGGCTACCACGTCCTGCTCCCCTGGCAGC22170.17004063487972904No Hit
GGTCAAAGCAGTGACCCTTCACTAGTCATCCACCTTACCAGAGCCTGGAT22050.16912025255291047No Hit
GTACAATGGGCAGCATCTCGTATGCTCAGCAGATTCCACCTTATGGACAG21740.1667425982086292No Hit
TCTTCCAGGAGAGCTGTTCCATGATCTTCCTGGGCACAGAGGTGAGGCTC21530.16513192913669672No Hit
GGGAAGGGAGTGCACTGACCACGGGTTAGCAGCCTGAGGGAAGCACAGGC21260.16306106890135497No Hit
TATGCAGGCTGCTGCTGCTGCGGCTGTGACTGAGGGTATGAAGATGACTG20630.1582290616855575No Hit
TCCTTGAAGTGCTGCGCAGCTCCCTCATCACATCCAAACCCTGGAGAGGC20570.15776887052214825No Hit
CCTCTGTATCACCCAAGGCCGTGTTTGTCACACCCACACCTCAGCCTGTC20510.15730867935873896No Hit
ATTCAGTGGATAAGGAATCGGTTGGAAGGTCACAGCCAAAGGCTTGTGGT20200.15493102501445766No Hit
GCATATGGGCAGCAAAGCCAGACTCCATATTACAACCAGCAAAGCCCTCA19990.1533203559425252No Hit
TCCCAGACCCCTCACTCTCAGCCTTCTTATCAGCAGCAGCAACAGCCTCA19950.15301356183358567No Hit
CTTCAAGGAAAGGACCTGCAAAGCCACTGAAGGACTGGCAGCTGTGTAAT19790.15178638539782757No Hit
GTAATGGGCCCTCCAAGCTCTGCCAAGCATTTGGGATTGATAAGGCTTTT19540.14986892221695558No Hit
GTACAGGTTTGTTCAGAAGGAACATCTCAGCAGAAGCACTTCAGTTGCAT19450.14917863547184168No Hit
GTGCAGAACCTTGCATTTTGCTTAGTTGAATCTCATTAGTTCCACACGGG19440.14910193694460677No Hit
AGCGAAAGCAGGGAATATGCAGGCTGCTGCTGCTGCGGCTGTGACTGAGG19380.14864174578119752No Hit
TTCCAGGAGAGCTGTTCCATGATCTTCCTGGGCACAGAGGTGAGGCTCAC19010.14580390027350695No Hit
GTATTGGGAACAGAGGGGAATGGTCACAGAAACCTCTGAGGTTTTATTTA18870.14473012089221862No Hit
TCTCCAGGGTTTGGATGTGATGAGGGAGCTGCGCAGCACTTCAAGGAAAG18740.14373304003816517No Hit
AACCTGTACTAACACGTGGCTCTTTTTCTGCACAGGGGAAGGGGCTGCAG18730.14365634151093032No Hit
ACCTTATGGACAGCAGGGTCCCAGTGCATATGGGCAGCAAAGCCAGACTC18350.14074179747600488No Hit
AAAGCAGGGTGATGAATCACACTGGAGAAGAAAACAGGAGTTTTCTTCAC18300.14035830483983047No Hit
CTTATCAGCAGCAGCAACAGCCTCAGTCTCAAGCTCCACAGCTTCAGACA18200.13959131956748166No Hit
GCTTCAGACATCGCAACCTGCGTATTCTCAGCAGCAGTCTCAGCCACCCC18160.13928452545854214No Hit
TTCCTCATGTGCCTTCACACATCTTCCAGGAGAGCTGTTCCATGATCTTC18080.1386709372406631No Hit
TTTCTGCACAGGGGAAGGGGCTGCAGTCTTACTCCGCTCTCTGGAGCCTC18070.13859423871342824No Hit
CCCTGGAGAGGCTCCAGAGAGCGGAGTAAGACTGCAGCCCCTTCCCCTGT18070.13859423871342824No Hit
GACTGTGACTGCTGCTGTGAGTAGGGAGGCTGATTCTGTTGGTGCTGCTG17840.13683017258702598No Hit
GGATTGGTATTGGGAACAGAGGGGAATGGTCACAGAAACCTCTGAGGTTT17790.13644667995085158No Hit
TCCCTGAACCTGAGCCTCCAGGTGGGGAACTGCCCCCCACTGTAAGGGCA17700.13575639320573765No Hit
GACCACGGGTTAGCAGCCTGAGGGAAGCACAGGCCCTTTGGCTGGATGCT17480.1340690256065703No Hit
GTCTCAAGCTCCACAGCTTCAGACATCGCAACCTGCGTATTCTCAGCAGC17260.13238165800740295No Hit
GGTATGAAGATGACTGCTGAGAAAGAGCCGAGGAAGCTGTCTGCTGAGTT17200.13192146684399367No Hit
TCCCTATGCTGAATGTCCAGCATCCCACAGCTCTGTGTCCTTGGTTCATC16940.12992730513588677No Hit
GGAGTGCACTGACCACGGGTTAGCAGCCTGAGGGAAGCACAGGCCCTTTG16780.1287001287001287No Hit
GTGTGTATTCATTCCTCAACATCGTGGTGATATAAGAAAAACCTCTTGCC16620.1274729522643706No Hit
CCCAAACCCAGTGGATTTATAATAAACTCTGATGATAAGGTCTCCAGCCA16600.12731955520990088No Hit
AGCGAAAGCAGGGTGATGAATCACACTGGAGAAGAAAACAGGAGTTTTCT16590.12724285668266597No Hit
CATATGGGCAGCAAAGCCAGACTCCATATTACAACCAGCAAAGCCCTCAT16390.12570888613796838No Hit
GGCTGTGACTGAGGGTATGAAGATGACTGCTGAGAAAGAGCCGAGGAAGC16350.12540209202902886No Hit
GCCAGGGGCAGGGAATGCAACTGAAGTGCTTCTGCTGAGATGTTCCTTCT16330.1252486949745591No Hit
GGAGAAGAAAACAGGAGTTTTCTTCACTTTTTGTCACAGATGGGGCTGTC16180.1240982170660359No Hit
CAGCAGACACCATCCCAGACCCCTCACTCTCAGCCTTCTTATCAGCAGCA16110.12356132737539173No Hit
GGATATGGAGTTGGGGACTGCTGATGGGGTGGCTGAGACTGCTGCTGAGA15990.12264094504857317No Hit
GCTGATGGGGTGGCTGAGACTGCTGCTGAGAATACGCAGGTTGCGATGTC15950.12233415093963365No Hit
AAAGCAGGGAATATGCAGGCTGCTGCTGCTGCGGCTGTGACTGAGGGTAT15940.12225745241239878No Hit
GGTTATGTCAGATCACCTGCTCGTTCCTCATGTGCCTTCACACATCTTCC15840.12149046714004998No Hit
ATGTTGAGGAATGAATACACACATGGTTCAGTTTTGACATAACTATCCAA15670.12018659217705702No Hit
CTCCAAGCTCTGCCAAGCATTTGGGATTGATAAGGCTTTTGACCAAAGGG15440.11842252605065477No Hit
AGCAGGGAATATGCAGGCTGCTGCTGCTGCGGCTGTGACTGAGGGTATGA15350.11773223930554085No Hit
CTCCATATTACAACCAGCAAAGCCCTCATCCCCAACAGCAGCAGCCTCCA15300.11734874666936645No Hit
GATGATAAGGTCTCCAGCCAGCAAATGAGAGGAGAAAGAAGTCCAGATGC15140.11612157023360838No Hit
GTCTTTGCTGAAGCGATGCTCCCAGGGCTGTGCAGCCCCATGCTACCCCA15140.11612157023360838No Hit
GTGTATTCATTCCTCAACATCGTGGTGATATAAGAAAAACCTCTTGCCCT15120.11596817317913863No Hit
GGCATGATCTTCCCTTGGTGAAGCTGTGCTGGTTATGTCAGATCACCTGC15090.11573807759743396No Hit
CTGTGGGTGCATCTCAAGATGTGAACCTTCAGGAAATTTGTTGATGACAC15020.11520118790678982No Hit
CTACAGAACAGACAGGTCAGAAAGATCCTCTGCTAAATGAAAAAGGAGAA14990.11497109232508518No Hit
GCTGTGGACTGCTGCTGAGGATATGGAGTTGGGGACTGCTGATGGGGTGG14850.11389731294379685No Hit
TTCTAGTACTGTAGCCACTTATAGAGGCAGAGGATTGCTTGTTCATTTTC14800.11351382030762246No Hit
ATCCAGATGGCTGCATCTTGAGTCAGGTCCCTTTGGTCAAAAGCCTTATC14730.11297693061697829No Hit
CTGCTGGGCTGTGGACTGCTGCTGAGGATATGGAGTTGGGGACTGCTGAT14730.11297693061697829No Hit
TACCAATCCTGGTCGTGGCTACCACGTCCTGCTCCCCTGGCAGCTCCTGT14580.1118264527084551No Hit
GAATCACACTGGAGAAGAAAACAGGAGTTTTCTTCACTTTTTGTCACAGA14410.11052257774546213No Hit
GATAAGGCTTTTGACCAAAGGGACCTGACTCAAGATGCAGCCATCTGGAT14370.11021578363652261No Hit
GGGTTTGGGAGCAGCTTTGGATAGTTATGTCAAAACTGAACCATGTGTGT14350.11006238658205286No Hit
GTGATGAATCACACTGGAGAAGAAAACAGGAGTTTTCTTCACTTTTTGTC14310.10975559247311333No Hit
GAATTTGGACAAGTCCCTGGGGCTGGATGTCATGCAAGCCAGGGTCCTAA14210.10898860720076453No Hit
GAATACACACATGGTTCAGTTTTGACATAACTATCCAAAGCTGCTCCCAA14200.10891190867352966No Hit
CTTGCAAGATTCATCAGCTGCAAGAGATGGGTTCCATTCCTGCCATCTCT13930.1068410484381879No Hit
CCTTACCAGAGCCTGGATCCATTCACAAGGGAGAAAGTGGCTTTCAGTGC13910.10668765138371814No Hit
GTAATGCTTCCACCCAGGGCTCCTTCAGAGCGGCCCAGCTGCTCCCCAGC13910.10668765138371814No Hit
GCTTGAGACTCTCCTAAAGAGCACAGCTCAAGTCCTGGTTGGTGGCACAC13900.10661095285648325No Hit
CTCCAGTGCTGTCACTCTATCATAAAATGGCATCAGATTGGTCAGGCATG13830.10607406316583909No Hit
CCCTTCCCCTGTGCAGAAAAAGAGCCACGTGTTAGTACAGGTTTGTTCAG13720.10523037936625541No Hit
GCTGAGAAAGAGCCGAGGAAGCTGTCTGCTGAGTTTGCTGCTGCTGCTGC13710.10515368083902053No Hit
GCCTTTAACATGGTCCCCTATATTCTTATCTCTAAATTGGAGAGAGAGGG13630.10454009262114147No Hit
GCTCAAGTCCTGGTTGGTGGCACACTTGCAAGATTCATCAGCTGCAAGAG13580.10415659998496708No Hit
ATGGAGAACAGCTCAGCAACCACATCAGCCAGCTCCTTTAGGACCCTGGC13390.10269932796750436No Hit
GTCCCAGACCAACTCATGAGACTGAATTTGGACAAGTCCCTGGGGCTGGA13340.10231583533132997No Hit
GTTGTAGACAAGAAAATGGAGCGAGAGATGGCAGGAATGGAACCCATCTC13200.10124205595004165No Hit
TCTTGAGTCAGGTCCCTTTGGTCAAAAGCCTTATCAATCCCAAATGCTTG13130.10070516625939749No Hit
GTTCCACACGGGCCCAGATTTAGAGTTTATCAGGGTCCCTCTAGATGGCA13090.10039837215045795No Hit
CTTCTGATGGTACCCAGGATATCATTGGCCTTCCAGTCTGCAGGTGCACA13070.1002449750959882No Hit
TGATAAGGCTTTTGACCAAAGGGACCTGACTCAAGATGCAGCCATCTGGA13040.10001487951428356No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)