FastQCFastQC Report
Mon 10 May 2021
HGJM2BGXJ_n01_ReSeq_1971_d02_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGJM2BGXJ_n01_ReSeq_1971_d02_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences505639
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATAGGGAACTTGGTTTGGTGAGATTAAATATTTTGTGCCTAGAAATCC15580.3081249666263876No Hit
ATATAGAACCCCCACTTTGACAGACCTTGATAGAGATGCCAGGCTTGATT13900.27489968139324694No Hit
CCATTAAGGGAGATCTAGTTGAACTTGCTCTCCAGAAAAAATATTTCCAA11400.22545729265345432No Hit
TCCCTATAGAGGTCAAGCTCTTCTACCAAAACTTGTGTTCAGTTACTTTT9500.18788107721121194No Hit
GTGTTAACAGACTGCTCAGGTGAACATGGGAGAAGTGTTTCAACACATAC9320.18432122522194688No Hit
ATCTATAGCTGAAAGGTTTGAGACTCATTCTGTATGGCTATCGGTGATGT8640.1708728954847233No Hit
CCCTTAATGGAGCCTGTGAACTCCCCAAAAACCAATAAAGGACTACAGCT7510.148524935774337No Hit
ATTAATTACAGCCATCCAGCTTGAGATCCTCCAGAAAAGCAGAGCTCACA7440.14714054888962283No Hit
CTCCAAAACAATCTAGAGACGGAACTGGGATTTTAATGTACTTCATCTAT7300.14437177512019445No Hit
CTTAAGGGCTGGTAGGTATTTGGAGCTGGAAGCTGCTTCCTTCACTTGAG7230.14298738823548027No Hit
ATATAGAAGGGTTCCTGCATACCAAAAATATGCCTGAAAGGACAACCATT6650.13151675404784838No Hit
GTTCTAAGTGATCCAAAGCAAGTTGCTACAAGGAAACAAAACAATTCCTA6510.12874798027842No Hit
GCCCTATTAATTACAGCCATCCAGCTTGAGATCCTCCAGAAAAGCAGAGC6500.12855021072346082No Hit
GCATATGGGTATGACTAAGGAGCAGAGATGCTCTTCGATCTTTACATCTC6450.12756136294866496No Hit
CTATAAAAGCAGTAAGTAGTTCACACCTGTCACTATCAGTGATGACACTG6310.12479258917923657No Hit
GTATTTTGTCAGCTTTTTTGTTTGTTTTAAACCGTTCTGCAATGCAAGCC6280.12419928051435906No Hit
CAATTAGCACATAGCCCTATTAATTACAGCCATCCAGCTTGAGATCCTCC6070.12004611986021647No Hit
TTATTTTACTGTCTCCAAAACAATCTAGAGACGGAACTGGGATTTTAATG5900.11668403742591059No Hit
GAACTGGGATTTTAATGTACTTCATCTATAGCTGAAAGGTTTGAGACTCA5880.11628849831599225No Hit
ACATTAAGTAACGTCTTGTTGTTGGCTACATTTACATTACAGAGCTTGAA5690.112530876771768No Hit
CCCATATGCAAATCATTTCCTAGAACGGTTTGGTGTACAAGGTGAGCTGT5640.11154202899697215No Hit
AATTACAGCCATCCAGCTTGAGATCCTCCAGAAAAGCAGAGCTCACAGCT5500.10877325522754375No Hit
CCATTGTGGAGCTGCTCTCTCTCTTTTTTAGCAGGGAACCCTGAGCGTAC5490.10857548567258458No Hit
CCTTTGGAGTATGCCTTGTTCTTTGGTGATGGAATGTGTGATTTTTTTCT5450.10778440745274791No Hit
ATCTAGAGACGGAACTGGGATTTTAATGTACTTCATCTATAGCTGAAAGG5340.10560894234819702No Hit
CTCTTATTCAAAAGTAACTGAACACAAGTTTTGGTAGAAGAGCTTGACCT5290.10462009457340118No Hit
CCTTAGTCATACCCATATGCAAATCATTTCCTAGAACGGTTTGGTGTACA5210.10303793813372783No Hit
AAGCAGGGGAGTGCTTCCTTTGGAGTATGCCTTGTTCTTTGGTGATGGAA5200.10284016857876864No Hit
TGACTAAGGAGCAGAGATGCTCTTCGATCTTTACATCTCAAATAACATCA5130.10145578169405446No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)