FastQCFastQC Report
Mon 10 May 2021
HGJM2BGXJ_n01_ReSeq_1970_d10_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGJM2BGXJ_n01_ReSeq_1970_d10_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences136
Sequences flagged as poor quality0
Sequence length151
%GC43

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGAAAGAGTCATCAAAGTTTACAGAATTCTTTGTCTAACAGAATTGC21.4705882352941175No Hit
CTATAAAACCTTCAGCTCTCATAAGAACTCACTCATTATCATGAGAACAG21.4705882352941175No Hit
GTTTTGGAGGGAGTGGAGATCTCCCATTCTCATCACTGCTTCTCCAAGTG10.7352941176470588No Hit
GGTGACACTTGTCTTGCCCCCGGCTGCTGTTCGTGCCACGGCAGGCTATA10.7352941176470588No Hit
GGACAACAGCAATTAGGGTAAAAAGACTTAACAATTGTCTTGCTCATCAT10.7352941176470588No Hit
ATACAACTATAACAAGGAAACGAAGAGAATCACAGTTCTCGGAAAGGATG10.7352941176470588No Hit
CTATGAGAGTAAGCCCTCAAAAAATGTTGTAAAAAATCTTATTCTGGGGG10.7352941176470588No Hit
CCTGGAGCCTACCTGTGAGATTGAAGTAGAGGAAGACGGCGGGAAGCCGG10.7352941176470588No Hit
AAGAATCAGCAGCACAGAGACTTGAAGTAAATTGCTTAAGTAACCCAGCA10.7352941176470588No Hit
AACATGTATATTTCTGCTTAAACAATAAACGAAACCTGTGTCTGAACTGT10.7352941176470588No Hit
CTATACAATAAAATGGAATATCAACCATTTCAGTCTTTAGTAACTAAGCC10.7352941176470588No Hit
CAAGAAGCCTATTTTGATCTCTCTCAGTTTGTAGCACACGTTCAAGGCTG10.7352941176470588No Hit
GTTCTGGGAACTCAGGTAGCTTAGGGAGGGCCTGCCTACCACTGGTAGAG10.7352941176470588No Hit
CTGAAGAAGATGTGATGAACATTATTAATTTAGAACAACCTAAAGGTGTT10.7352941176470588No Hit
GAAAAAGAAAAAATCCAAACACTGTAACAAGAGCCAATGAAGGGCACTAC10.7352941176470588No Hit
CTCAAAAAACGGTCAATGCTAACCACAAACCTCTCCGTGCTGGAGTACTC10.7352941176470588No Hit
ACAAAGAGCTGGTTCTTTGAAAAGAACAAAGCTGACAGAGTCTCAGCCAG10.7352941176470588No Hit
GAGTAAATACAAAAACAACCATAAAATGAGCAACAAAATGGCAAGAAATG10.7352941176470588No Hit
GTTATATTCAATCTTTGAAGATTGACCGAAAGCACATCTTGGGGACTATT10.7352941176470588No Hit
ATAACAACCTCTGCCTACTTCGCACTGAGAAGTCAATGACAACCAATTAT10.7352941176470588No Hit
GTACTTGTTGTTGGTCCATTTCTGTTCCACCACGTTACAGCCAGAGGTGA10.7352941176470588No Hit
GCTTAGACCCCCACCATATCTCTTGTATAATTTGCACATAATGACGTATC10.7352941176470588No Hit
ATCAAGAGTCACAGAGCTCCACCGACTAATGGACTAGACTAATCACCTAA10.7352941176470588No Hit
CAACGGGTGCCATCGTTGGGCAGAATGGGCCCTCAGCGGCCGCTGGTGAC10.7352941176470588No Hit
TTCCAACACTGTGTCAAGCTTTCAGGTAGACTGCTTTCTTTGGCATGTCC10.7352941176470588No Hit
ACCAAACAGAACATTAATCTTATCCACAAGAAGTATGCTATTTGGGATTC10.7352941176470588No Hit
ATGCATAAACTTTTAAGACATTTTCAGAAGGATGCGAAAGTGCTTTTTCA10.7352941176470588No Hit
GATCAGGGGTGCGATCTCAGGAACCTGGGATTATGTCCTGAGCCAAAGGC10.7352941176470588No Hit
GGCTAAGGCCATGGAGCAGATGGCTGGATCAAGTGAGCAGGCATCAGAAG10.7352941176470588No Hit
CTCCTGCTGGGTCTCCCCGGACAGTCCCCCAGACAGGCAACTACCTGAGC10.7352941176470588No Hit
GCTGCACTGCTGGGTGGGTAAATATTTATAAATGTTGCATCGTTTTACAG10.7352941176470588No Hit
GTCCTAAGCCAGACAGAGAGATAGAGTGACTGGGCCTGAAACCATGTAGG10.7352941176470588No Hit
GAATTCAAGGAGGATAGATCCCAAGTGGACTAGGGAGGAGACCCTAGGCT10.7352941176470588No Hit
TAATCAACCAGAAAAAAAGAACATCAAAAACTATCAAAAAATCAAAAGAA10.7352941176470588No Hit
TCATTATCCAAAAATCTACAAAACTTATTATCAAAGAGTCGAAAGGCTAT10.7352941176470588No Hit
TGATTCAGCTGATAGTGAGTGGGAGACACGAACACTCGATTGCCGAAGCA10.7352941176470588No Hit
GCGTACGACCATCGCCCGTCACGGACGTCTCCGTACCCTGGGCCCCCTCG10.7352941176470588No Hit
CAATTGGGACTCACCCTAGTTCCAGTGCAGGTCTAAAAGATGATCTTATT10.7352941176470588No Hit
GTTGGGCATCAGCAATCTGATCACAAGTGGCACATACTAGGCCAAGAGCC10.7352941176470588No Hit
CCATTACTGCCTGGTCCATTCTAATGCAAAGGGGCCCTGTCACTTTCTGC10.7352941176470588No Hit
GACTGTTCAGCTGTCTACTAATGACCAGTAGAGAGATTAAATAAGAAATC10.7352941176470588No Hit
GATTTTTCTCATGTACTACAGGGAGCAGAAATTCTAAGATTAGGAATGTG10.7352941176470588No Hit
AACCAGGGGTCCTGTTCTTCATATATTTTAGATATTAACCCCTTCTCCAA10.7352941176470588No Hit
GCAATATCCCAATGATACTTGCTGCAACAACGAGAGGATCACTTGAATCT10.7352941176470588No Hit
GATTTGAATATATCAATGACATATTTATGTGTGACTGCTTCAGTTCTCAG10.7352941176470588No Hit
TCCTCCCTCCAGGACCCCCCATTTCTGTAAATCACAGAATCTGGGCTTGA10.7352941176470588No Hit
CTCAATCAGAGGAGTGAGAAACATCAAAATCTTTGTAGATGAGTACTCCA10.7352941176470588No Hit
CCTTATCGAGACATCCCAAGTATCTATGATGAACACAAACATAAAAATGC10.7352941176470588No Hit
ACCATGGCATTCAGCATGATTACGAAAGATAATAACAAAAATACTTAAAC10.7352941176470588No Hit
CTGTTTAACAAACATCATATCTAAAAACAATCCCACAAGCAGTTAGGGTT10.7352941176470588No Hit
GGAAGTGAATATGGAGCCCGGGAAAGCAACGCCCAAAAGGCCCCAGCTCT10.7352941176470588No Hit
GCCCTGTGTTGGCACATGAGTTGGCCAGGATCGGCTGCGGGGGGGCCCTC10.7352941176470588No Hit
AAAATCGACATGCACAAAGGATCCAACCTTTAGCAAATCGACTAATTCAA10.7352941176470588No Hit
TTGCTGATTCTCACTCCTCTCATTGAAAACTCGAAGCTTGGACTCATGTC10.7352941176470588No Hit
GGTCAGAGCTGAGCTCAGGATCCTCGACAGGGAGGGAACGGGGTGCAGAA10.7352941176470588No Hit
CTCAATAAATGTCCTGCTTGTAATGGTGTATTGGACCCATTTAATGGGAA10.7352941176470588No Hit
TCCGGAATCTTCACATTCCAGAAGTTTGCTTGAAGTGGGAGTTGATGGAT10.7352941176470588No Hit
GAGTGGAGATCTCCCATTCTCATCACTGCTTCTCCAAGTGAATCTCTGTA10.7352941176470588No Hit
CTTCCCGTATTGTAGAATTAGGATTCCACATAGTTGGGTGACGAGTTTTA10.7352941176470588No Hit
ATTCATAGAATTCACAGGTGAAACCATGGGGTCCTGGACTTTTGCTTGTT10.7352941176470588No Hit
GTTCTTCCAGGCAGGTCCCAGACCTGCACCTCTGGCTTTGGTACTCCTCC10.7352941176470588No Hit
CTCTAGTCGCAAGTCCAGTTCCACCACGACGGACTCCACCTGGCTCCACT10.7352941176470588No Hit
GCCTGCTTTAAACTAAACTAAACTAAACTAAACTAAACTAAAATAACACC10.7352941176470588No Hit
ATGCGGGGCTCGATCCCAGGACCCTGCGATCATGAACTGAGTCAAAGGCA10.7352941176470588No Hit
TGCTGCCAGTATGCTTGCAATTTGGCAACAACGAGTCGCGCCTCTTTTTT10.7352941176470588No Hit
TTCCAGTGCAGGTCTAAAAGATGATCTTATTGAAAATTTGCAGGCTTACC10.7352941176470588No Hit
CTTTAGTTGAATGCTTACGAAAGGATTAAGAAGCAAGTGGTCACTTAATC10.7352941176470588No Hit
GTACCACATTCCCTTATACTGGAGATCCCACAAAAAGCCAAGGAAAATGA10.7352941176470588No Hit
GGCTCCTTCTATTCTCCGGGGGCTGAGTCCTGCACCTTAACCACACTGGG10.7352941176470588No Hit
ATCCAGGAGAGCCAGTGCCCACCATGCCTCCGCTGGAAAAAACTGTTGAT10.7352941176470588No Hit
GAGCAAAAGCAGGGAGAGTAGAAGAGGGAGAAGCAGGCTTCTCGCTGAGC10.7352941176470588No Hit
ATTTAAAAGGAAAAAAAGGTTTACAGATATGTGCACAAGTATGCACTTGG10.7352941176470588No Hit
GAACAACTTTTTTTTTTTTTTAATGTTGGCTCTAAGGGCTGCCTGTGTGG10.7352941176470588No Hit
GGACAAGAAGCAACAGTTTTTGTATATATCACCACGATATAAAATAAAAT10.7352941176470588No Hit
TTATTAAATGGTATCATAAAAGGCATGTTTCGTGTTTTTTTCTATCAATC10.7352941176470588No Hit
GGGCTGGACTTTATATTGCAGTGGAAGTGATGCTGAGACCACGGGCATCT10.7352941176470588No Hit
GAACTAGGGTGAGTCCCAATTGTCCTCATAGCCTGCACCATTTGCCTAGC10.7352941176470588No Hit
ATGTACCCTCAAAATTCTTGTGTACCCAGAATCTCAGAATGTGAACTTAT10.7352941176470588No Hit
CTACAGATTCAGTGCAAATATCAATAGCATTTGTCACAGAACTAGAACAA10.7352941176470588No Hit
CTTTTTTTCTTGGTGATAGATTCTCACTCTGTTGCCGGCTGGAGTGCAGT10.7352941176470588No Hit
CACTTCTCTCAATGATCCCCCATCCAGACAGAAGATCACAACGGAAACAT10.7352941176470588No Hit
GCTACGAAAATGAGATTTAAATGGAAATTTAGTTATGCTCCAGTTGATTC10.7352941176470588No Hit
GGCCATGGACACAAGGGATGCAACCGTCCGAAGACATGGGAAGCGGGAAA10.7352941176470588No Hit
CCAAAGAACCAGAACGAACAACGCCCACAACAGAGGAGAATAATCGATAT10.7352941176470588No Hit
GTGTGTACATTGAAGTGTTGCATTTGACTCAAGGAACATTCTGGGAACAG10.7352941176470588No Hit
GTAAACTCAGCTTTGTGGGTGTTACCCTAAGAGATTAGAAGTAAATTAGG10.7352941176470588No Hit
GACCAAAAACAAATACAGTTCATTAAACAAAAAACAACATAAATTATATT10.7352941176470588No Hit
GAACACATCAGGAAGACAGTAGAACAACCCATCACTTAGGATCAAATGGA10.7352941176470588No Hit
GCATTCCTTCCTCTTGTTTTTCTGGCAGAGTTGGAGAAGGATTGGTATTC10.7352941176470588No Hit
CACAAAAACCACCCTTGAACATAAGTCCAAAATCCATAAGTCCACATCAC10.7352941176470588No Hit
TTATCACACTGTGTTATGACCGTACTGAGAATTTCTCCCCTAGAATACAA10.7352941176470588No Hit
ACAGCAACAACATTTACAAATCTACAAAGACGTGCAAGTATCATTAGAAG10.7352941176470588No Hit
TCTCTGTGCATTTACACATCTCTAAAACGAACACATTTAGATGAAAGCTG10.7352941176470588No Hit
TTTAGATACTGAATATTGTCTTTCTCATCTCTTTAACCCAGGAAAGCAAT10.7352941176470588No Hit
TTCCGTAGAAGGCCCTCTTTTCAAACCGTATTTAAAGCGACGATAAATGC10.7352941176470588No Hit
GTTCTGAGGGGATTCCTAATTAAAGGAAAACAATCCAAGAAATAATGAAA10.7352941176470588No Hit
TATTTACAGAGGAAAAAAAAAAAGAAAACCAGACTTAGAGCAGGGGTGTG10.7352941176470588No Hit
CCCCTGGCTAGCTGTGTGGCCCTGGGCAATCACAGTCCTTCCCCGGACTC10.7352941176470588No Hit
GTAGCCAATGAGATATTTTCTAGCTTTTGTCCCTCGTAAGCAAAATCAAA10.7352941176470588No Hit
GTGTAGATTTGCTTCTCTGGGCCTGGTGTCGTCGGCGATAGAATTTTATG10.7352941176470588No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA10.7352941176470588No Hit
GCGACGCACACAGTAGGGGCTTAATAATGGCCGACTTGTCTGACGCAATG10.7352941176470588No Hit
GTATAACAAAGGTTACCAAAGACTAAGGGGAGAGACAGGTAGGAAGTTAT10.7352941176470588No Hit
GCCCTGTGGAGGGCTTGGTAAGGCAGAGGCTGGGGAGGCTGTGTGCCTCA10.7352941176470588No Hit
CCTCCCAGGTTCATGCCATTCTCCCGCCTCGGCCTCCCAAGTAGCTCGGA10.7352941176470588No Hit
ATTTATTATTCATCTCTTGGCAGTCTGTTTCCTGTCTTCACTATAATTGT10.7352941176470588No Hit
GAATATTAATGAGGTTGTACAGTCACAAGATGGGGTTGATACAGGACTTA10.7352941176470588No Hit
GATTGAATCATAAAAGCACTCAGAGGTGATCTGAATTTAGTCAATATGTA10.7352941176470588No Hit
CTGAGGGCAGAAGCTTCCATGCAAACCGCAGTGCTGCCACTTACTGGCTC10.7352941176470588No Hit
CTCTCTGGCTGACAGTAACTATTCAACATTAAATTTTAATTAACATATAA10.7352941176470588No Hit
CTCTCTCTCTGCCCCTGCTGCCCTCTCTCCCTCTGTAAAATAAAAGAAGT10.7352941176470588No Hit
ATTGATGTGTTTTTTCATCTAATTTGCAAAATTTAAGCAATTCTTAAAAT10.7352941176470588No Hit
CCCCGCCCTGCTGGGGGCCTCTAGGTGACACTTTCGCAGAGAGGAAGTAC10.7352941176470588No Hit
CAAGAGGATTGTATGATAAAAGCAGTCAGAAGTGATCTGAATTTCGTCAA10.7352941176470588No Hit
GAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCA10.7352941176470588No Hit
CACGAAGGGGGTGGGGGCTGGTCGGGATGAAGCGGCAGAGCGCCGAGGAA10.7352941176470588No Hit
GGCGCAGTGGGGTGGCAGTGGTACAGGGTGTGGGGACCAGCCAGGTGGCA10.7352941176470588No Hit
CCTGTAGAACTCAACTTAGGAACTGGAGCTTTTAGCTTGCTTCCAAAATG10.7352941176470588No Hit
GCCTGGCCCGGCTTCTCCGAGCGCAGACGTCCCGAAGAGGAATCGGCCGC10.7352941176470588No Hit
GAATGAGTAATCACCTCAGAACAGCGGACTCCACTCCAGCTGTGCCATCA10.7352941176470588No Hit
TTTGTTTCCTTTATGACAGAGGCCACTCATGATTATCAATACATCTTGTG10.7352941176470588No Hit
CTACAGTTGGAAATCTGATCTGTGGTACATCCCTTTCAGCTGATAACAAA10.7352941176470588No Hit
CTCTCTCTCTGTGTATTCACAGGTGTATGCATATGCCCCTGGGGCAGGGC10.7352941176470588No Hit
ATAGATCACTCTAAAAAATCTAATTTTAAAAATAGATCACCTGAAAGTAA10.7352941176470588No Hit
GCATGGTGGTGCATGCCTGTAATCCCAGCTAGTTGGGAGGCTGAGGCAGG10.7352941176470588No Hit
TTCTTAGACCATGAACCAATACTGTCGGTAAGCATCCCATCATAAATGAT10.7352941176470588No Hit
GAAGGGGCCTGCCGTGCAGCCCATGCCAGTAGCCGCTCTTCTGTGCCTTT10.7352941176470588No Hit
CATTGTGATACTCTAGTGTCTGTGTCACCTCTGATTTCTTCCAGAAAGTC10.7352941176470588No Hit
TTGTGGGTTCGAGCACTGAAGTGTCTGGTGCTTCATTTCCAAGCAGAGAT10.7352941176470588No Hit
TATCTAATACAGTGAATTATTCCACACATCCGTTCTAGTATTAAGAATGC10.7352941176470588No Hit
ATGCAGGGCTCGATCCCAAGACCCTGGGATCATGACCCAAGCCGAAGGCA10.7352941176470588No Hit
CCTCCCAAGTATCTGTGATGAACATAAACATAAAAATCCTCACCCAAATA10.7352941176470588No Hit
ACATTTATCTGTTCAACTTCAGTGAAAGTTGGGAGGAAAAGGTGCCAGAA10.7352941176470588No Hit
CATATGATCATTTCAGTGGATTCAAAAAATTTTTATGACTAATTTCCCCA10.7352941176470588No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)