FastQCFastQC Report
Mon 10 May 2021
HGJM2BGXJ_n01_ReSeq_1970_d04_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGJM2BGXJ_n01_ReSeq_1970_d04_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences212209
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATAAAGACTATCCAATACTCAGTGTGGACAGATAATGATTATGTCCAG9440.4448444693674632No Hit
GCCCATAACTGTGCACTTTTCTCCAAGCATCTCCCACCACTCGTTGCTGC8580.4043183842344104No Hit
GTTATGGGCTTCCTTTGTACCTATGGGCTCAACCAGGTGGCCGTACTGGA7500.3534251610440651No Hit
GTCTTTATTCTGTGCCCACATACCGCACAATTCAGCTTCTTCTTTGCCAA7350.34635665782318376No Hit
GTACAAAGGAAGCCCATAACTGTGCACTTTTCTCCAAGCATCTCCCACCA7140.3364607533139499No Hit
ATGTATAACCTTCCTTTAGTGGATGTCTCCTGTACAGTTATGGACCCATT6610.311485375266836No Hit
GTGTGGGAGCACAGCTCCCAGGGAGGCCAGTGGGGAACAAGAGCTAGGAG6290.29640590172895587No Hit
GATAATGATTATGTCCAGTGCTGTATCCCGCAGCCTGCAGCACACTCAGG5840.27520039206631197No Hit
CTTTTGCACCAGTGAGAGTCACAGCTTCCCAACCTCTGCTCTCCCCAGCC5510.2596496849803731No Hit
ATTTACACCAGCTTTGCTGTGGCTGTGCATTCTGGTTGGCAAAGAAGAAG5370.25305241530755057No Hit
ATCCAATACTCAGTGTGGACAGATAATGATTATGTCCAGTGCTGTATCCC5360.25258118175949185No Hit
GAAGCAGACTGAGAGGTAGATGTTCACCTCCAAGTCCACTTGAGTCGCAG5280.2488113133750218No Hit
CCCACATACCGCACAATTCAGCTTCTTCTTTGCCAACCAGAATGCACAGC5020.2365592411254942No Hit
GTATGTGGGCACAGAATAAAGACTATCCAATACTCAGTGTGGACAGATAA5020.2365592411254942No Hit
CTCCTGTACAGTTATGGACCCATTTTTTGTCAGTGTGAGATCTCAGGGAG5020.2365592411254942No Hit
GTTATGGACCCATTTTTTGTCAGTGTGAGATCTCAGGGAGCTAAGAAAAG4820.22713457016431915No Hit
CTCAGAAGCAGACTGAGAGGTAGATGTTCACCTCCAAGTCCACTTGAGTC4650.21912359984732033No Hit
GTGCTGTATCCCGCAGCCTGCAGCACACTCAGGTTATGGCCTGCAGGGTG4490.21158386307838029No Hit
GTATTGGATAGTCTTTATTCTGTGCCCACATACCGCACAATTCAGCTTCT4420.20828522824196902No Hit
ATCTGGGGCCATAGGCATGGCGCTGGACTGGACCTGCCACATCTGTTGGC4360.20545782695361647No Hit
GTCCAGTGCTGTATCCCGCAGCCTGCAGCACACTCAGGTTATGGCCTGCA4330.20404412630944022No Hit
CTCCAGTACGGCCACCTGGTTGAGCCCATAGGTACAAAGGAAGCCCATAA4110.19367698825214766No Hit
TTATAACATCTTCTGCCCACTTTTGCACCAGTGAGAGTCACAGCTTCCCA4010.1889646527715601No Hit
ATCATTATCTGTCCACACTGAGTATTGGATAGTCTTTATTCTGTGCCCAC3940.18566601793514884No Hit
ATTATGTCCAGTGCTGTATCCCGCAGCCTGCAGCACACTCAGGTTATGGC3870.18236738309873757No Hit
GCTGTATCCCGCAGCCTGCAGCACACTCAGGTTATGGCCTGCAGGGTGAG3800.1790687482623263No Hit
CCAATACTCAGTGTGGACAGATAATGATTATGTCCAGTGCTGTATCCCGC3730.17577011342591503No Hit
GGTTATGGCCTGCAGGGTGAGCAGGGGCCTGCAGAGCTGCTGCTCATTGC3540.1668166760127987No Hit
GTACAGTTATGGACCCATTTTTTGTCAGTGTGAGATCTCAGGGAGCTAAG3490.16446050827250494No Hit
ATATGAAACTGCTCTGTGGAAGTTCAAACCAAAAGAAGCTGTGGGGGTAG3250.15315090311909485No Hit
GTCTAAAGCAGAATAAGCTGTAAGAACCAAAGATGTTTGCCCACATGATA3250.15315090311909485No Hit
AAGCAGGAGAGATGAGTGGTGTGGGAGCACAGCTCCCAGGGAGGCCAGTG3230.15220843602297734No Hit
ATTCTACTCTCTGCCCACAAGGACAGCATTGGGAATTCATAGTTAAGAAC3210.15126596892685984No Hit
CCTCCAAGTCCACTTGAGTCGCAGTCTGGCCAGCCCCTCCAGTACGGCCA3190.15032350183074233No Hit
GTCAATGAGTGGCCATGGCCTCTCTTCCTCCCTGTTTTGCAATTTATAAC3160.14890980118656608No Hit
ATACAGCACTGGACATAATCATTATCTGTCCACACTGAGTATTGGATAGT3150.14843856763850732No Hit
CTTCTTGGAGCCACAGGAGCAGGGGTTCTGCATATTCTTGAAAAAAGGCA3110.1465536334462723No Hit
GTGTAGGAAGAGCCTGAGGATGAGGCACGTATGTTTTGTCATCATGTGAG3060.14419746570597855No Hit
CCCTGTAACTGCACCAGAAGATAATTGCAGTAATGGCTCAATATGAAACT3020.14231253151374354No Hit
CCTTATAACTGCCCTATGCCCTGGTTCAGGAGAACATTTGTATTGGGGTC2940.1385426631292735No Hit
GCTCCCAGGGAGGCCAGTGGGGAACAAGAGCTAGGAGGATTTGCTGCTGA2860.13477279474480347No Hit
ATTTATAACATCTTCTGCCCACTTTTGCACCAGTGAGAGTCACAGCTTCC2840.13383032764868596No Hit
CCTTTGTACCTATGGGCTCAACCAGGTGGCCGTACTGGAGGGGCTGGCCA2810.1324166270045097No Hit
ATGCAGAACCCCTGCTCCTGTGGCTCCAAGAAGAGATCGAGAGGATCTGC2770.1305316928122747No Hit
GAGTATTGGATAGTCTTTATTCTGTGCCCACATACCGCACAATTCAGCTT2710.12770429152392218No Hit
GCTTTGAGGAGTAAGGTATGAAAATTTTGTGGGTATTTATTTTTGTTATC2700.12723305797586343No Hit
CCCCTGGGTCAATGAGTGGCCATGGCCTCTCTTCCTCCCTGTTTTGCAAT2690.12676182442780468No Hit
GGACTGGACCTGCCACATCTGTTGGCAAAGTTGGGATGATGTCACCCACA2680.12629059087974592No Hit
GACTGAGAGGTAGATGTTCACCTCCAAGTCCACTTGAGTCGCAGTCTGGC2670.12581935733168714No Hit
GTATTGGGGTCGGACTTAAACTTTTTGGAAGTTGCTAAATCCTGCTGGCT2660.1253481237836284No Hit
GTCTTAAATGTAGTTGATTAGGTATTCTGTTCTTTTTCTCATTGCTTGTT2640.1244056566875109No Hit
ATTCAGCTTCTTCTTTGCCAACCAGAATGCACAGCCACAGCAAAGCTGGT2600.12252072249527587No Hit
CTCTTCCTCCCTGTTTTGCAATTTATAACATCTTCTGCCCACTTTTGCAC2580.12157825539915838No Hit
GTCCACACTGAGTATTGGATAGTCTTTATTCTGTGCCCACATACCGCACA2520.11875085411080587No Hit
GGACCATACTGTTCAAGAATCATTTTTCCCTCTGAGAAAAGGAGCTTGGG2510.1182796205627471No Hit
GCCTACCCCTGGGTCAATGAGTGGCCATGGCCTCTCTTCCTCCCTGTTTT2510.1182796205627471No Hit
ATACCGCACAATTCAGCTTCTTCTTTGCCAACCAGAATGCACAGCCACAG2500.11780838701468835No Hit
GGTGTAAATAGGGGGTCATGTGGGTGACATCATCCCAACTTTGCCAACAG2460.11592345282245334No Hit
TTTGTATTGGGGTCGGACTTAAACTTTTTGGAAGTTGCTAAATCCTGCTG2410.11356728508215957No Hit
ATATAATTTTGAAGGAAAACAAAACCCCACAGTAACATCTTTTTCCTAGT2370.11168235088992455No Hit
TTGCTGCAGATCCTCTCGATCTCTTCTTGGAGCCACAGGAGCAGGGGTTC2370.11168235088992455No Hit
AAATTATATTTACTTATTTTTATTTTCTCTCAGAAGCAGACTGAGAGGTA2350.11073988379380703No Hit
CCCTAGAACTTCCTGCAGAGGAAATCCTCTACCCTTCCATTGAATTTATC2350.11073988379380703No Hit
GTAGATGTTCACCTCCAAGTCCACTTGAGTCGCAGTCTGGCCAGCCCCTC2340.1102686502457483No Hit
CCATAATCCTATCAATGTATAACCTTCCTTTAGTGGATGTCTCCTGTACA2340.1102686502457483No Hit
GTGTGAGATCTCAGGGAGCTAAGAAAAGTCCTTATAACTGCCCTATGCCC2340.1102686502457483No Hit
GATGAGTGGTGTGGGAGCACAGCTCCCAGGGAGGCCAGTGGGGAACAAGA2330.10979741669768954No Hit
CATGATACTTTGCCACATTTCCTTTGAAACACGTTCTTAAAAAACAGAAG2310.10885494960157203No Hit
GCACTGGACATAATCATTATCTGTCCACACTGAGTATTGGATAGTCTTTA2280.10744124895739578No Hit
GGTACAAAGGAAGCCCATAACTGTGCACTTTTCTCCAAGCATCTCCCACC2270.10697001540933702No Hit
CTCCAAAGGTGTAATTATGAAGGCTATGCCATTTTCTCTTTCCCACCCTT2260.10649878186127827No Hit
ATCTCAGGGAGCTAAGAAAAGTCCTTATAACTGCCCTATGCCCTGGTTCA2220.10461384766904326No Hit
GAGCAGGGGTTCTGCATATTCTTGAAAAAAGGCAGCCCGGAGCTCGGGAG2210.10414261412098451No Hit
GTATAACCTTCCTTTAGTGGATGTCTCCTGTACAGTTATGGACCCATTTT2210.10414261412098451No Hit
GCCCTATGCCCTGGTTCAGGAGAACATTTGTATTGGGGTCGGACTTAAAC2200.10367138057292574No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)