HGJJWBCXY_l02n01_transplanta2_29.35100000083813.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	HGJJWBCXY_l02n01_transplanta2_29.35100000083813.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8305412
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAG	45730	0.5506048345343976	No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACGATATCTCGTATGCC	31420	0.37830754211831996	TruSeq Adapter, Index 7 (97% over 35bp)
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTA	27804	0.3347696658516158	No Hit
TTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAA	19117	0.23017521587128972	No Hit
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAG	17340	0.20877952833646302	No Hit
CTGACATGTGTGCGAGTCAACGGGTGAGTAAACCCGTAAGGCGCAAGGAAG	14892	0.17930477139484471	No Hit
CCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACC	14380	0.17314011634823173	No Hit
CCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTA	14260	0.17169527532168183	No Hit
CGGCAATCGGGCGGCGGGCGCATGCGTCGCTTCTAGCCCGGATTCTGACTT	13704	0.16500084523200054	No Hit
CAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAG	13380	0.16109977446031576	No Hit
CGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGT	13053	0.15716258266296723	No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAG	12642	0.15221400214703376	No Hit
TTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT	11497	0.13842781068536997	No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCAC	11180	0.1346110223069006	No Hit
TCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAAC	10927	0.13156481580925788	No Hit
GATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGG	10125	0.12190846161514925	No Hit
CACGCTTTCACGGTTCGTATTCGTACTGAAAATCAGAATCAAACGAGCTTT	9767	0.11759801921927533	No Hit
GGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTC	9697	0.11675519528712122	No Hit
CTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGA	9681	0.11656254981691457	No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGTAAGTAT	9584	0.11539463665378671	No Hit
CCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAGCGGAGTCCTATAA	9465	0.11396183596912471	No Hit
CTGAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCC	9329	0.11232434947236813	No Hit
CCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAG	9235	0.11119255733490403	No Hit
GTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATC	9209	0.11087950844581823	No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTG	9056	0.10903733613696706	No Hit
CCCGACTGTCCCTGTTAATCATTACTCCGATCCCGAAGGCCAACACAATAG	9004	0.10841123835879544	No Hit
TATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAG	8468	0.1019576151068725	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
GCACACG	3830	40.359985	11
CGTATGC	3830	39.595543	44
CACGATA	3870	39.35952	34
GTATGCC	3865	39.353413	45
TCGTATG	3920	38.686462	43
ACGTCTG	4005	38.65169	15
CTCGTAT	3970	38.029205	42
CACACGT	4115	37.783417	12
CACGTCT	4165	37.383858	14
AGCACAC	4255	36.646008	10
ACGATAT	4155	36.605183	35
AGTCACC	4210	36.128925	28
CGATATC	4235	35.914135	36
TCGGAAG	4390	35.10926	3
CAGTCAC	4365	34.89712	27
ACTCCAG	4475	34.441345	23
GAGCACA	4540	34.395103	9
TCCAGTC	4480	34.202217	25
TATCTCG	4415	33.991463	39
CCAGTCA	4580	33.406322	26

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content