FastQCFastQC Report
Tue 28 Mar 2017
HGJJWBCXY_l02n01_transplanta2_28.35100000083806.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGJJWBCXY_l02n01_transplanta2_28.35100000083806.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3102271
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAG168810.5441497535192766No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTA111190.358414851571639No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCGTATGCC110630.35660972236145716TruSeq Adapter, Index 2 (97% over 36bp)
TTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAA77430.24959134775782No Hit
CCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACC63330.20414077300145603No Hit
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAG62620.20185212703854694No Hit
CGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGT60240.19418032789527412No Hit
CCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTA59270.19105358622763777No Hit
CAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAG53240.17161621276800124No Hit
TTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT46020.14834293973672835No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAG46020.14834293973672835No Hit
TCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAAC44470.1433465999585465No Hit
GGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTC42690.1376088678261828No Hit
CTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGA40620.1309363366385464No Hit
CCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAG39500.12732607821818273No Hit
GTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATC38710.12477955665381908No Hit
GATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGG36880.11888065227054632No Hit
TATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAG35340.11391654694254628No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG13750.037.8031811
CGTATGC13150.037.6415144
GTATGCC13550.036.69636545
ACGTCTG14250.036.15685715
TCGTATG13800.035.54245843
CTCGTAT13750.035.5080742
CACGTCT14800.034.81599414
CACACGT14850.034.6987712
AGCACAC15500.033.5350810
AACTAAT15400.032.43361335
AGTCACC15450.032.03946728
ACACGTC16150.031.76635613
TCCAGTC16250.031.70831525
CAGTCAC16450.030.638927
TCGGAAG16800.030.1340373
CCAGTCA16700.030.04550426
CTAATCT16550.030.04445637
GAGCACA17150.029.6526419
TCTCGTA16800.029.32951541
ACTAATC16900.029.28910336
Produced by FastQC (version 0.11.2)