FastQCFastQC Report
Tue 28 Mar 2017
HGJJWBCXY_l02n01_transplanta2_27.351000000837eb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGJJWBCXY_l02n01_transplanta2_27.351000000837eb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2296164
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAG156160.6800907949083776No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAAAGATCTCGTATGCC121980.5312338317297893TruSeq Adapter, Index 7 (97% over 37bp)
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTA101410.4416496382662562No Hit
TTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAA67840.2954492797552788No Hit
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAG54760.23848470753831172No Hit
CGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGT51090.22250152863645625No Hit
CCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACC48860.21278967878601007No Hit
CAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAG46360.2019019547384246No Hit
CCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTA44390.19332242818892725No Hit
TTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT41030.17868932706897242No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAG40770.17755700376802355No Hit
GGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTC39640.1726357524985149No Hit
TCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAAC39050.17006624962328476No Hit
CCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAG34400.1498150828947758No Hit
GATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGG33010.14376150832431828No Hit
CTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGA32940.1434566520509859No Hit
TATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAG31260.13614010149100847No Hit
GTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATC30790.13409320937006242No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTG27920.1215941021634343No Hit
CTGACATGTGTGCGAGTCAACGGGTGAGTAAACCCGTAAGGCGCAAGGAAG27710.12067953334343713No Hit
CGGCAATCGGGCGGCGGGCGCATGCGTCGCTTCTAGCCCGGATTCTGACTT23660.10304142038634873No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC13350.040.2802644
GTATGCC13650.039.7246445
GCACACG14050.039.5562511
ACGTCTG14300.039.02120615
TCGTATG13900.038.52456343
CACACGT14550.038.35157412
CACGTCT14850.037.72831314
CTCGTAT14550.036.80353542
AGCACAC15350.036.20620710
AGTCACC15250.034.96741528
GAGCACA16100.034.938859
CAGTCAC15650.034.2174527
TCGGAAG16050.034.2058073
CTGAACT16350.033.8526719
GATCTCG16150.033.57531739
ACTCCAG16450.033.51010523
TCTCGTA16250.033.09177841
CCAGTCA16550.032.6285926
TCCAGTC16350.032.47725325
GAACTCC16900.032.35155521
Produced by FastQC (version 0.11.2)