FastQCFastQC Report
Tue 28 Mar 2017
HGJJWBCXY_l02n01_transplanta2_23.35100000083764.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGJJWBCXY_l02n01_transplanta2_23.35100000083764.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5658342
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAG437800.7737248826599735No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTA263770.46616128894294473No Hit
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAG185140.32719832063880194No Hit
TTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAA182740.32295679547118217No Hit
CGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGT139450.24645028526024051No Hit
CCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACC139270.24613217087266903No Hit
CCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTA136590.24139580110216033No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAG135910.24019403563800135No Hit
CAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAG130910.23135752487212685No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTATG128140.2264620979078324TruSeq Adapter, Index 7 (97% over 36bp)
TTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT109730.19392606526788236No Hit
TCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAAC105540.1865210692460795No Hit
GGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTC104250.1842412494684839No Hit
GATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGG102630.18137821998034054No Hit
GTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATC94950.16780533944395726No Hit
CTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGA93620.16545482758023464No Hit
CCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAG92800.1640056398146312No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTG92680.16379356355625022No Hit
TATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAG83230.14709255820874736No Hit
ATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGT66370.11729584390621846No Hit
TGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCTAGGTTA61420.10854769824800267No Hit
GTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATA61380.10847700616187568No Hit
TTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGAT59950.10594976408283559No Hit
CGGCAATCGGGCGGCGGGCGCATGCGTCGCTTCTAGCCCGGATTCTGACTT56780.10034741625727112No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG18500.035.1491711
AGTCACG18150.034.3396628
TCGTATG18450.033.53708645
ACGTCTG19450.033.4320815
CACGAGT19000.032.68325431
CACACGT20100.032.3512312
GTCACGA19200.032.2273329
ACGAGTG19350.032.20836332
CTCGTAT19350.031.9772244
CGAGTGG19950.031.46524433
TACTCTA36100.031.2262294
AGCACAC21250.030.81222510
TCTAGTT37350.030.360297
CACGTCT21450.030.31513814
GTACTCT37200.030.1214163
TCACGAG20700.029.99965330
ACACGTC22050.029.49023413
TCGGAAG22350.028.993723
TTTTTCC75500.028.823151
CAGTCAC22700.028.05107927
Produced by FastQC (version 0.11.2)