FastQCFastQC Report
Tue 28 Mar 2017
HGJJWBCXY_l02n01_transplanta2_21.35100000083721.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGJJWBCXY_l02n01_transplanta2_21.35100000083721.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6868049
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAG375490.5467200365052725No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTA278470.40545721208453817No Hit
TTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAA186500.2715472763808179No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTATG146860.2138307399961765TruSeq Adapter, Index 21 (97% over 40bp)
CCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACC121470.17686245395162442No Hit
TTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT107970.15720621678732927No Hit
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAG99580.1449902293941118No Hit
TCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAAC97430.14185979162350182No Hit
CAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAG90160.13127454390613694No Hit
CCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTA81980.11936432020214184No Hit
CGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGT78570.11439930029619766No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAG75390.10976916443083036No Hit
TATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAG74290.10816754510633224No Hit
CCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAG73050.10636208332235253No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG21200.035.97948528
TCGTATG21150.035.53080445
ACGTCTG21850.035.42352715
GCACACG22250.034.68708811
GTCACGT21900.034.62397429
CGTTTCG22250.034.2795833
CTCGTAT22300.034.0011844
CACGTCT24400.031.90729514
AGCACAC24350.031.69559110
CACGTTT24350.031.1384331
CACACGT25050.031.07936312
ACGTTTC25150.030.32686432
TTTCGGA26950.028.30152935
ACACGTC27650.028.07551413
TCGGAAG27400.027.9204643
GAGCACA27750.027.8932559
TTTTTCC67450.027.7944761
CAGTCAC28400.027.09580227
GAACTCC29300.026.41536321
GTCTGAA30550.025.99717317
Produced by FastQC (version 0.11.2)