FastQCFastQC Report
Tue 28 Mar 2017
HGJJWBCXY_l02n01_transplanta2_20.35100000083707.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGJJWBCXY_l02n01_transplanta2_20.35100000083707.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14259735
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAG882290.6187281881465538No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTA632710.4437038977232045No Hit
TTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAA419400.29411486258335096No Hit
CCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACC247220.17336928070542684No Hit
TTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT227570.1595892209778092No Hit
TCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAAC224370.15734514000435493No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATG206280.14465906975129622TruSeq Adapter, Index 20 (97% over 44bp)
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAG191550.13432928452036452No Hit
CAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAG190220.13339658836577256No Hit
CGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGT175010.12273019098882271No Hit
GGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTC174340.12226033653500573No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAG171820.12049312276841048No Hit
TATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAG171370.12017754888151849No Hit
CCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAG152240.10676215231208715No Hit
CCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTA152200.10673410129991898No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG31250.033.98544711
AGTCACG29800.033.2216128
TCACGTG30000.032.77432630
TCGTATG30150.032.3876245
GTCACGT30450.032.36454829
GCCTTAT30650.032.22620437
ACGTCTG33550.031.72132715
TTTTTCC149800.030.4828111
CTCGTAT32850.029.86250344
AGCACAC36750.029.14409410
CACGTCT36700.029.06077414
CACACGT38900.027.24370612
GTACTCT59250.026.3929733
CACGTGG38550.026.0303231
TCTAGTT62300.026.0025847
TTTTCCA176700.025.875282
GAGCACA42750.025.21169
TAGTTGT63300.024.5982049
TCGGAAG44200.023.8240413
TATCTCG41150.023.67517341
Produced by FastQC (version 0.11.2)