FastQCFastQC Report
Tue 28 Mar 2017
HGJJWBCXY_l02n01_transplanta2_19.351000000836ec.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGJJWBCXY_l02n01_transplanta2_19.351000000836ec.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14367752
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAG877010.610401682879827No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTA649420.45199833627417846No Hit
TTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAA430170.29939965556198356No Hit
CCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACC298380.20767340638953125No Hit
CGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGT292900.20385930937560728No Hit
TTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT250030.17402165627580432No Hit
CAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAG242620.1688642732697502No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATG236820.16482745526231243TruSeq Adapter, Index 19 (97% over 40bp)
GGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTC236350.16450033380308904No Hit
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAG230910.1607140769133543No Hit
TCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAAC223300.15541749328635404No Hit
CCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTA208470.14509576724319853No Hit
CCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAG204850.14257623600407357No Hit
CTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGA197070.1371613318492691No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAG194330.13525428334230713No Hit
TATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAG167730.11674060075647186No Hit
GTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATC165960.11550867526109859No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTGA33250.034.5098131
GCACACG35000.034.3946311
AGTCACG34500.033.52218228
ACGTCTG36550.033.11968215
TCACGTG35500.032.4502330
TCGTATG35150.032.1970345
GTCACGT35950.032.10751729
CTCGTAT36800.030.87569444
AGCACAC40250.030.299710
CACGTCT39950.030.13265214
ACGTGAA39000.029.47941632
TTTTTCC150050.029.277561
CACACGT43300.027.90530212
TGAAACG42900.026.90473435
GTACTCT60750.026.4824773
TCTAGTT62950.026.3771427
GAGCACA47500.026.0066039
ACACGTC49050.024.72578613
TTTTCCA181350.024.269242
TCGGAAG49300.024.1895523
Produced by FastQC (version 0.11.2)