FastQCFastQC Report
Tue 28 Mar 2017
HGJJWBCXY_l02n01_transplanta2_15.35100000083665.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGJJWBCXY_l02n01_transplanta2_15.35100000083665.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3559403
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAG385691.0835805892167871No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTA210290.590801322581343No Hit
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAG148530.41728907909556745No Hit
TTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAA147320.41388963261535716No Hit
CCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTA117780.3308981871398097No Hit
CGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGT116230.3265435242932593No Hit
CAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAG114940.32291932102096893No Hit
CCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACC109330.3071582509763575No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATG107080.300836966199107TruSeq Adapter, Index 15 (97% over 40bp)
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAG104480.2935323704565064No Hit
GATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGG90340.25380660745636274No Hit
TTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT89920.2526266342979427No Hit
TCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAAC87280.245209660159302No Hit
GGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTC85390.2398997809464115No Hit
GTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATC75020.2107656817730389No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTG72380.20334870763439825No Hit
TATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAG72100.2025620588621182No Hit
CCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAG69280.19463938194129746No Hit
CTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGA67750.1903409082927671No Hit
CGGCAATCGGGCGGCGGGCGCATGCGTCGCTTCTAGCCCGGATTCTGACTT58540.16446578260455477No Hit
TGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCTAGGTTA52530.14758092859954325No Hit
ATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGT52040.14620429324805312No Hit
TTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGAT51040.14339483334705286No Hit
CTGACATGTGTGCGAGTCAACGGGTGAGTAAACCCGTAAGGCGCAAGGAAG50490.14184963040150272No Hit
GTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATA44180.12412193842619114No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCAC42750.12010441076776078No Hit
ATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGT41570.11678924808458048No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGTAAGTAT38110.10706851682711961No Hit
TGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTA38040.10687185463404958No Hit
TTTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA37750.10605711126275952No Hit
CCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAGCGGAGTCCTATAA37630.1057199760746395No Hit
AAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAAC37410.10510189489641943No Hit
AGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTG37390.10504570569839942No Hit
CAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATC37200.10451190831720937No Hit
AGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGAT36350.10212386740135915No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG11900.041.02834745
GCACACG12500.039.96177311
ACGTCTG13300.037.72617315
CTCGTAT13050.037.5852344
AGCACAC13500.037.0016410
CACGTCT13700.036.4614714
CAGTCAC13600.036.2322127
ACTCCAG13900.035.93533323
GTCACAT14100.035.42561329
CACACGT14450.034.8804412
TCGGAAG14300.034.774273
TTTTTCC56450.034.7657781
ATGTCAG14150.034.6629334
CATGTCA14350.034.3366133
AGTCACA14700.033.67354228
GAACTCC14800.033.4450821
CACATGT14800.033.4446131
GAGCACA15100.033.3789639
GTACTCT27050.033.1068273
CTCCAGT15250.032.45954524
Produced by FastQC (version 0.11.2)