FastQCFastQC Report
Tue 28 Mar 2017
HGJJWBCXY_l02n01_transplanta2_13.35100000083608.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGJJWBCXY_l02n01_transplanta2_13.35100000083608.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1619351
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAG1282327.918727934833153No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATG675624.172165268678625TruSeq Adapter, Index 13 (97% over 40bp)
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTA560393.4605839005873342No Hit
TTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAA393692.431159149560534No Hit
CCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACC372222.2985751699291876No Hit
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAG329902.0372359050014484No Hit
CGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGT310051.9146559331485267No Hit
CTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGA285511.7631137412457212No Hit
CCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAG273991.6919741303769227No Hit
TTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT266051.6429421416357541No Hit
GTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATC262431.6205875069703852No Hit
TCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAAC246031.5193123664974426No Hit
CCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTA237021.463672792371759No Hit
GGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTC204571.263283871131089No Hit
CAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAG197171.2175865516493953No Hit
GATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGG178281.1009348807022072No Hit
TTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGAT177851.0982794959215143No Hit
GTTTGATCAATCTAGGTTACTCTGTAGGGTAAAAATTCTCGTGTTCCGTTT149380.9224683221858633No Hit
GTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATC143130.8838726131641627No Hit
TTTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA128120.7911811583776465No Hit
AGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGAT127580.7878464891181713No Hit
ATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGT127370.7865496732950422No Hit
CAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATC122700.7577109595140274No Hit
AAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAAC122560.7568464156319414No Hit
GTTAGATAGTAGGACTGTTTGATCAATCTAGGTTACTCTGTAGGGTAAAAA120870.7464101359124736No Hit
AACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAA114850.7092347489827715No Hit
TATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAG114610.7077526737563382No Hit
AGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTG110230.6807048008739304No Hit
TAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATT90390.5581865821554437No Hit
GTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATA88200.5446626457142398No Hit
ACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAA86160.5320650062895568No Hit
TACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCA84520.5219374922422625No Hit
TGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCTAGGTTA77860.48080990470873824No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTG77050.4758079008195259No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAG74340.45907280138771644No Hit
ATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGT73110.4514771658522458No Hit
GTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACT72400.44709269330738055No Hit
TCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTT71680.44264646762808063No Hit
CTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTATCAGGACTGT70130.43307473179069883No Hit
AGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGAT69090.4266524058094879No Hit
GAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGA65280.40312446158985915No Hit
TGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTA63580.39262642873595655No Hit
CAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAA62300.3847220275283123No Hit
TAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGA60910.37613834184188605No Hit
CGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTACTA58820.36323193674502935No Hit
GTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTG57500.35508052299964615No Hit
CTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGAT56710.3502020253793032No Hit
GGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTT55370.34192710536505055No Hit
GAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCC52670.32525375906767584No Hit
CTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACT52040.32136331159828846No Hit
CACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCT46880.2894986942299724No Hit
CTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGT45210.27918592077937393No Hit
GAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTACTATCT44750.2763452765953768No Hit
AAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGA43250.26708230643016867No Hit
GTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCTAGGTTACTCTG42670.2635006246329548No Hit
GTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGA42660.2634388714985201No Hit
CTACTATCTAACCTCTGTAGGACTATCAGGACTGTTTGATCAATCTAGGTT42490.26238906821312985No Hit
CAGAGGTTAGATAGTAGGACTGTTTGATCAATCTAGGTTACTCTGTAGGGT42040.2596101771635674No Hit
CCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCTAGGT41390.2555962234253105No Hit
CGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACTAG37120.2292276350216846No Hit
GCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCTA36470.22521368128342772No Hit
GATCAATCTAGGTTACTCTGTAGGGTAAAAATTCTCGTGTTCCGTTTGTAC35880.2215702463517792No Hit
CGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAATCTAG35630.2200264179909112No Hit
GTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGATAGTAGG35040.21638298305926262No Hit
GAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTACTAT34720.21440688275735156No Hit
GTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTATCAGG34540.2132953263375266No Hit
ACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAAT32720.20205625587040735No Hit
AACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACA32330.19964788362745323No Hit
GTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGATAGT31140.1922992606297214No Hit
TGGACACCTACTATCTAACCTCTGTAGGACTATCAGGACTGTTTGATCAAT31110.19211400122641725No Hit
GGACTGTTTGATCAATCTAGGTTACTCTGTAGGGTAAAAATTCTCGTGTTC30320.1872355036060743No Hit
GTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTATC29280.1808131776248633No Hit
TAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCA28660.1769844832899106No Hit
ACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAG28000.172908776417219No Hit
TTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTACTATCTAACC27320.16870956327565795No Hit
AGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCT26760.16525138774731357No Hit
TTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTACTATCTAAC26460.16339879371427196No Hit
GTAGGACTGTTTGATCAATCTAGGTTACTCTGTAGGGTAAAAATTCTCGTG26400.16302827490766364No Hit
GGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTT25290.15617367698540957No Hit
CTGGACACCTACTATCTAACCTCTGTAGGACTATCAGGACTGTTTGATCAA24200.14944258533202498No Hit
ATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTACTATCTAA23170.14308201248524874No Hit
ACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCTAGG22900.14141467785551126No Hit
GGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACTAGG22900.14141467785551126No Hit
CTAACCTCTGTAGGACTATCAGGACTGTTTGATCAATCTAGGTTACTCTGT21320.13165768261482533No Hit
AGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTACTATC20450.12628515991900457No Hit
CTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCTAGGTT19730.12183893423970468No Hit
AGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGT19700.12165367483640052No Hit
TAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTG19530.12060387155101025No Hit
ACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTA19380.11967757453448943No Hit
TAGGACTGTTTGATCAATCTAGGTTACTCTGTAGGGTAAAAATTCTCGTGT19300.11918354945901168No Hit
GGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAATCTAGG18570.11467557064527703No Hit
CACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTAC18540.11449031124197286No Hit
GCGTATAATGGGTGGTCTTTGAATAAAGTCTGAGTGGGCGGCAAAAAAAAA18080.11164966705797569No Hit
GATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCTAGGTTAC17720.10942655421832574No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17500.10806798526076188No Hit
AGACGTGTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16950.10467156286685221Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAATC16930.10454805659798277No Hit
TTAGATAGTAGGACTGTTTGATCAATCTAGGTTACTCTGTAGGGTAAAAAT16910.10442455032911332No Hit
TAGATAGTAGGACTGTTTGATCAATCTAGGTTACTCTGTAGGGTAAAAATT16680.10300422823711475No Hit
CCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCTAGGTTACTCTGTA16650.10281896883381059No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGGTG453.8380676E-1045.0129741
TCTGAAC77550.044.6771718
CACGTCT78100.044.5724514
ACGTCTG78100.044.54088615
GTCTGAA77900.044.5053217
CTGAACT77850.044.50519
CGTCTGA78200.044.4496616
TGAACTC77900.044.4475620
GAACTCC77600.044.44544221
CTCCAGT77400.044.33595724
TCCAGTC77300.044.3059825
ACTCCAG77550.044.305523
CACACGT78650.044.28936412
ACACGTC78650.044.26075413
CCAGTCA76450.044.23934626
AGCACAC78900.044.2074310
GAGCACA78900.044.207439
CAGTCAC76550.044.18155727
TCACAGT76250.044.1742330
TCGTATG75350.044.1616345
Produced by FastQC (version 0.11.2)