FastQCFastQC Report
Tue 28 Mar 2017
HGJJWBCXY_l02n01_transplanta2_11.351000000835c3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGJJWBCXY_l02n01_transplanta2_11.351000000835c3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1909543
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAG375681.9673817243183316No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTA182470.9555689502671583No Hit
CGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGT145280.7608103090634775No Hit
TTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAA128670.6738261458369883No Hit
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAG124190.6503650349848105No Hit
GGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTC116700.6111409902788258No Hit
CCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACC104250.5459421442722159No Hit
CCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTA94050.49252622224270415No Hit
CAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAG93210.48812726395792083No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCC87440.4579106100255402TruSeq Adapter, Index 11 (100% over 51bp)
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAG85950.4501076959251507No Hit
GTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATC83350.43649187266272604No Hit
TTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT82910.4341876564183158No Hit
CTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGA78510.4111454939742127No Hit
CCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAG76980.4031331056697859No Hit
GTTTGATCAATCTAGGTTACTCTGTAGGGTAAAAATTCTCGTGTTCCGTTT76170.3988912530380305No Hit
TCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAAC74560.3904599163255292No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTG69710.36506116908600644No Hit
GATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGG69350.36317590124967075No Hit
TGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCTAGGTTA67410.35301640235386167No Hit
TATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAG58760.30771760573079526No Hit
GTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATA51140.2678127698616894No Hit
ATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGT50410.2639898656380087No Hit
TTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGAT49780.26069064692442123No Hit
AAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAAC47120.24676061235594066No Hit
CAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATC43980.23031688733901254No Hit
GTTAGATAGTAGGACTGTTTGATCAATCTAGGTTACTCTGTAGGGTAAAAA43620.22843161950267685No Hit
TTTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA43080.22560371774817325No Hit
AGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGAT41650.21811501495383975No Hit
ATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGT40050.20973604679234767No Hit
GTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATC39540.20706525069087212No Hit
GTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCTAGGTTACTCTG38490.20156655283489294No Hit
CGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTACTA37340.19554416946882056No Hit
AGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTG36160.18936468044972016No Hit
CGGCAATCGGGCGGCGGGCGCATGCGTCGCTTCTAGCCCGGATTCTGACTT33810.17705807096252874No Hit
AACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAA33750.17674385965647277No Hit
GAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCC31440.16464672437331865No Hit
GAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTACTATCT30380.15909565796633016No Hit
GTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACT30080.1575246014360504No Hit
TACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCA29130.1525495890901645No Hit
TGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTA28850.15108326966190339No Hit
TCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTT28000.14663194282611075No Hit
ACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAA27380.14338509266353258No Hit
TAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATT26010.13621060117525502No Hit
CAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAA24220.1268366305445858No Hit
GAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTACTAT23770.12448004574916616No Hit
GAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGA23550.123327937626961No Hit
CCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCTAGGT22790.11934792775025228No Hit
AAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGA22270.11662476309776738No Hit
GTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGA21850.11442528395537571No Hit
TTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTACTATCTAACC21240.11123080234380688No Hit
TTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTACTATCTAAC20740.1086123747933406No Hit
CTACTATCTAACCTCTGTAGGACTATCAGGACTGTTTGATCAATCTAGGTT20170.10562736738580905No Hit
GGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTT19800.10368973099846403No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC9750.040.61557445
AGTCACG9700.040.36312528
CGTATGC9700.040.36101544
ACGTCTG10450.040.0490815
GTCACGG9950.038.8956729
CTCGTAT9950.038.6685242
CACGTCT10850.038.36624514
CACGGCT10200.038.15994631
GCACACG10950.038.01586511
CATCTCG10300.037.7894639
ACGGCTA10250.037.7533132
TCGTATG10500.037.28589243
ACACGTC11300.036.63925613
AGCACAC11450.036.55230710
GGCTACA10700.036.37676634
CGGCTAC10700.036.37581633
CACACGT11500.036.00205612
CCAGTCA11000.036.00205226
TCCAGTC11800.035.65881725
CTACATC11150.035.51436
Produced by FastQC (version 0.11.2)