FastQCFastQC Report
Tue 28 Mar 2017
HGJJWBCXY_l02n01_transplanta2_10.351000000835aa.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGJJWBCXY_l02n01_transplanta2_10.351000000835aa.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13325179
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAG2953202.216255406400169No Hit
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAG1284130.9636868667955606No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTA1255840.9424563827622878No Hit
CGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGT1150150.8631403750748864No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCC905910.6798482782107467TruSeq Adapter, Index 10 (100% over 51bp)
TTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAA905410.679473048729777No Hit
CCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTA869080.6522088746425095No Hit
GGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTC857660.6436386332971588No Hit
CAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAG787860.5912565977537713No Hit
CCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACC785280.589320413631967No Hit
GTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATC751260.5637897997467801No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTG691190.5187097299030654No Hit
CTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGA688080.5163758025314331No Hit
GATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGG681650.5115503514061612No Hit
CCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAG633410.4753482110821926No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAG583570.43794533641912053No Hit
TTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT565770.42458716689659476No Hit
TCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAAC556100.4173302287346384No Hit
ATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGT484170.3633497156023195No Hit
TGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCTAGGTTA459270.3446632874500222No Hit
TATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAG457280.34316987411576233No Hit
TTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGAT438310.3289336676077672No Hit
AAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAAC384550.28858899381389175No Hit
GTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATA370260.27786493524777417No Hit
GTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATC363800.27301697035364403No Hit
CAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATC347680.2609195718871769No Hit
GTTTGATCAATCTAGGTTACTCTGTAGGGTAAAAATTCTCGTGTTCCGTTT340510.25553878113006967No Hit
TTTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA330940.24835688886430718No Hit
AGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGAT330770.24822931084077746No Hit
AGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTG322470.24200050145667837No Hit
AACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAA300550.22555044101096128No Hit
ATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGT292850.219771907004026No Hit
CGGCAATCGGGCGGCGGGCGCATGCGTCGCTTCTAGCCCGGATTCTGACTT278860.20927298612649028No Hit
TCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTT273690.20539311329326235No Hit
GTTAGATAGTAGGACTGTTTGATCAATCTAGGTTACTCTGTAGGGTAAAAA261940.19657522049047146No Hit
TACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCA249010.1868717861125918No Hit
ACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAA246640.18509319837279484No Hit
CGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTACTA239850.17999758202122462No Hit
TGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTA239210.17951728828558325No Hit
GTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCTAGGTTACTCTG237460.17820398510218885No Hit
GTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACT230820.1732209375949096No Hit
GAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCC222450.16693959608347475No Hit
TAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATT208150.1562080329277378No Hit
GAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTACTATCT206800.15519491332911925No Hit
GAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGA206340.15484970220662703No Hit
CAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAA191620.1438029462868754No Hit
AGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGAT177400.1331314198480936No Hit
AAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGA174340.13083501542455828No Hit
GTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGA160770.12065128731103725No Hit
GAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTACTAT156030.11709411183144332No Hit
CTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGAT153470.11517293688887782No Hit
TAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGA153360.11509038640306446No Hit
CCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCTAGGT150210.11272644067295456No Hit
GTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTG145140.10892161373592055No Hit
GGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTT144630.10853887966533132No Hit
CTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTATCAGGACTGT140260.10525937400165505No Hit
CTACTATCTAACCTCTGTAGGACTATCAGGACTGTTTGATCAATCTAGGTT139260.1045089150397154No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC99450.041.8544944
GCACACG103550.041.69935211
GTATGCC100650.041.46740745
ACGTCTG105550.040.84311715
CACACGT105750.040.8316912
TCGTATG102650.040.52779843
TAGCTTA103300.040.40286634
CACGTCT107450.040.10191714
CTCGTAT105050.039.68771442
TCACTAG105450.039.62296330
CACTAGC105550.039.45589431
ACACGTC109250.039.441213
AGCACAC111250.038.9345510
TCTCGTA108450.038.50556641
TCCAGTC110700.038.39509225
CCAGTCA109050.038.35704426
ACTCCAG112050.038.13316323
GAACTCC113350.037.77295321
CAGTCAC110800.037.73091527
TCGGAAG113800.037.665253
Produced by FastQC (version 0.11.2)