FastQCFastQC Report
Tue 28 Mar 2017
HGJJWBCXY_l02n01_transplanta2_05.35100000083509.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGJJWBCXY_l02n01_transplanta2_05.35100000083509.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4078231
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAG145090.355766997994964No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTA85210.2089386304993513No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC68900.16894580027467793TruSeq Adapter, Index 5 (100% over 51bp)
TTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAA60010.1471471331565083No Hit
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAG53340.1307920027090177No Hit
CGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGT48240.11828658062772805No Hit
CCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACC45130.11066072520168671No Hit
CCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTA43940.10774279338271912No Hit
CAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAG43100.10568307680462435No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC9450.033.57068644
GTATGCC9500.033.15716645
GCACACG10400.032.45319711
ACGTCTG10550.031.7769415
TCGTATG10350.030.65187343
CACGTCT11050.030.13692714
CACACGT11300.029.66930612
CTCGTAT12100.026.404742
CACAGTG12100.026.03216433
TACTCTA14850.025.6070654
AGCACAC13300.025.54611610
ACACGTC13200.025.05779813
TCGGAAG13450.024.9266283
ACACAGT12650.024.9003332
CACACAG12800.024.6085331
TCTAGTT16450.024.2088957
GTACTCT16700.023.5787683
GAGCACA14900.023.4069589
TAGTTGT16750.022.971059
TCTCGTA13800.022.66282341
Produced by FastQC (version 0.11.2)