FastQCFastQC Report
Tue 28 Mar 2017
HGJJWBCXY_l02n01_transplanta2_04.351000000834ee.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGJJWBCXY_l02n01_transplanta2_04.351000000834ee.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4984940
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC123760.24826778256107396TruSeq Adapter, Index 4 (100% over 51bp)
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAG115240.23117630302471043No Hit
CTGACATGTGTGCGAGTCAACGGGTGAGTAAACCCGTAAGGCGCAAGGAAG78550.15757461473959566No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTA78120.15671201659398107No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGTAAGTAT59030.11841667101309143No Hit
CCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAGCGGAGTCCTATAA57040.11442464703687508No Hit
TTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAA56150.11263926947967277No Hit
CACGCTTTCACGGTTCGTATTCGTACTGAAAATCAGAATCAAACGAGCTTT55490.11131528162826433No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCAC54200.10872748719142056No Hit
CCCGACTGTCCCTGTTAATCATTACTCCGATCCCGAAGGCCAACACAATAG51800.10391298591357168No Hit
CGGCAATCGGGCGGCGGGCGCATGCGTCGCTTCTAGCCCGGATTCTGACTT51480.10327105240985848No Hit
CTGAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCC50780.1018668228704859No Hit
CTTGCTTTGAGCACTCTAATTTCTTCAAAGTAACAGCGCCGGAGGCACGAC50250.10080362050496093No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG17150.037.1308511
CGTATGC16850.036.720344
GTATGCC17250.036.12967745
ACGTCTG18300.035.04130615
TCGTATG17550.034.74286343
CACTGAC18150.033.96520631
CACACGT19200.033.4000812
CTCGTAT18450.033.29198542
ACTGACC18850.033.1813332
AGCACAC19500.032.6561110
CACGTCT19850.032.30637714
TCGGAAG20900.029.9294033
AGTCACT21350.029.40366728
CAGTCAC21350.029.19288827
CCAGTCA21650.029.10015726
CTGAACT22000.029.0439719
TCCAGTC21850.028.83379625
GTCACTG21800.028.58971229
GAGCACA22350.028.4919079
CTGACCA22000.028.43036533
Produced by FastQC (version 0.11.2)