FastQCFastQC Report
Tue 28 Mar 2017
HGJJWBCXY_l02n01_transplanta2_02.351000000834ab.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGJJWBCXY_l02n01_transplanta2_02.351000000834ab.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6026700
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAG218120.36192277697579106No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTA108850.1806129390877263No Hit
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAG88670.1471286110143196No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAG77770.12904242786267775No Hit
TTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAA75080.1245789569747955No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC64900.10768745748087677TruSeq Adapter, Index 2 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC11250.031.59956444
GCACACG12150.031.11274511
GTACTCT24100.030.5293833
ACGTCTG12950.029.36423515
TCGTATG12350.029.14939543
CTCGTAT13100.027.65228842
GTATGCC12900.027.55775845
TAGTTGT26700.027.4733549
TCTAGTT27050.027.4481057
CCGATGT13350.026.9652533
TACTCTA27600.026.5763824
CACGTCT14550.026.44468714
CGATGTA13450.026.43042234
AGCACAC14500.026.38069710
TGTACTC30350.024.4656642
CACACGT15600.024.3762812
TGTTACC29950.024.1915913
CTAATGC29400.024.18313821
CTCTAGT34550.023.7038576
TTTTTCC47600.023.0255621