FastQCFastQC Report
Tue 28 Mar 2017
HGJJWBCXY_l02n01_transplanta2_01.3510000008349e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGJJWBCXY_l02n01_transplanta2_01.3510000008349e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2742694
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAG206780.753930259810245No Hit
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAG83670.30506502001316954No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTA81730.29799168263028974No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCC76160.27768318303099065TruSeq Adapter, Index 1 (100% over 51bp)
CGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGT75000.2734537648020523No Hit
CCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTA63830.23272738409753332No Hit
TTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAA59710.21770565728440722No Hit
CAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAG57730.21048647789363306No Hit
GGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTC55230.20137135240023132No Hit
CCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACC51640.18828203219170642No Hit
GTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATC50160.18288587789961258No Hit
GATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGG49850.18175560233843077No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTG47900.17464580445357739No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAG43380.15816565756150705No Hit
CTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGA40600.1480296380128443No Hit
TTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT40480.147592111989161No Hit
CGGCAATCGGGCGGCGGGCGCATGCGTCGCTTCTAGCCCGGATTCTGACTT39830.14522217936087659No Hit
CCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAG39570.14427420630956278No Hit
TCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAAC37120.13534138332602907No Hit
TATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAG32720.11929876245764201No Hit
TGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCTAGGTTA31960.11652776430764787No Hit
ATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGT30570.11145975453331651No Hit
TTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGAT28880.10529792969977694No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC8900.035.3937944
TCGTATG9250.035.0275543
GTATGCC9100.034.61590245
ACGTCTG9300.034.59918615
GCACACG9450.034.04999511
ACACGTC9750.033.002313
CTCGTAT9950.032.11103442
AGCACAC10250.031.39243110
CACACGT10400.030.93965712
GTCACAT10350.030.87002829
CACGTCT10650.030.21337514
GTACTCT14250.029.2098983
CATCACG11450.028.4923233
TTTTTCC37050.028.4245871
ACTCCAG11300.028.27476123
TCACGAT12050.027.26032835
GAGCACA12200.027.1125459
TCTAGTT16350.026.8337967
CAGTCAC12000.026.81290227
CTCTAGT15900.026.744226
Produced by FastQC (version 0.11.2)