FastQCFastQC Report
Tue 28 Mar 2017
HGJJWBCXY_l01n01_transplanta1_29.351000000837f8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGJJWBCXY_l01n01_transplanta1_29.351000000837f8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2551367
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAG85480.3350360806579375No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTA44710.17523939127534377No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACGATATCTCGTATGCC44670.17508261257592497TruSeq Adapter, Index 7 (97% over 35bp)
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAG38150.14952768457066348No Hit
TTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAA32300.1265987997806666No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAG29420.11531073342251427No Hit
CCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTA28890.11323341565521543No Hit
CGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGT28660.11233193813355742No Hit
CAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAG28000.10974508959314751No Hit
CCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACC27190.10657032092991718No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC5050.034.30670544
GCACACG5700.032.76230211
CACGATA5950.029.87492434
CACACGT6500.029.42230812
ACACGTC6600.028.29471613
GTATGCC6250.028.07981545
ACGTCTG6600.027.95381515
TCGTATG6300.027.1426843
TACTCTA11900.027.0371074
AGCACAC7000.026.99929410
CTCGTAT6500.026.30752242
CACGTCT7100.025.98523514
TCTAGTT12500.025.0193467
ACGATAT7150.024.8609535
GTACTCT12550.024.7403893
TAGTTGT13100.024.7321789
ACTCTAG14600.023.269945
CTAATGC13000.022.84555621
TATCTCG7600.022.79679739
GTTACCT13650.022.74665814
Produced by FastQC (version 0.11.2)