FastQCFastQC Report
Tue 28 Mar 2017
HGJJWBCXY_l01n01_transplanta1_27.351000000837c1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGJJWBCXY_l01n01_transplanta1_27.351000000837c1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4792171
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAG277670.5794242317312968No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTA146780.3062912404419625No Hit
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAG110800.23121044720649575No Hit
TTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAA101280.2113447120313528No Hit
CCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTA84530.17639186915491956No Hit
CCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACC82570.17230186485415483No Hit
CGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGT82500.17215579327198466No Hit
CAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAG80590.1681701258156272No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAG80220.16739803316701346No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAAAGATCTCGTATGCC70640.14740709377858177TruSeq Adapter, Index 7 (97% over 37bp)
TTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT63650.13282080293044635No Hit
TCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAAC62100.12958636075382118No Hit
GATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGG61620.12858472704751145No Hit
GGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTC59690.12455732485339109No Hit
CGGCAATCGGGCGGCGGGCGCATGCGTCGCTTCTAGCCCGGATTCTGACTT55970.11679466362949067No Hit
GTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATC55810.11646078572738745No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTG54350.11341414987069535No Hit
TATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAG51710.10790516448599184No Hit
CCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAG51700.10788429711711038No Hit
CTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGA51540.10755041921500713No Hit
CCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAGCGGAGTCCTATAA48020.10020510536873581No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC10600.033.96251744
GCACACG11800.032.60549511
GTATGCC11150.031.88364445
ACGTCTG12200.030.98351115
TACTCTA24700.029.6956484
TCGTATG12200.029.50841543
GTACTCT25400.029.4973263
TCTAGTT25750.029.4455917
CACACGT13350.028.81953812
CTCGTAT13050.027.24158342
TAGTTGT27350.027.2296989
CACGTCT14100.026.96800414
TTTTTCC54500.026.5951231
ACTCTAG33500.025.5892095
CTCTAGT33500.025.5889426
AGTCACC14550.025.36074828
ACACGTC15000.025.34939613
AGCACAC15650.025.30317310
GTTACCT29200.024.73451414
TTGTACT29700.024.5528091
Produced by FastQC (version 0.11.2)