FastQCFastQC Report
Tue 28 Mar 2017
HGJJWBCXY_l01n01_transplanta1_26.351000000837a8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGJJWBCXY_l01n01_transplanta1_26.351000000837a8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5934216
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAG449140.7568649338008593No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTA185680.3128972723608308No Hit
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAG177960.2998879717219596No Hit
CGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGT140440.23666142250298944No Hit
TTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAA124320.2094969242777816No Hit
CCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTA122800.20693550757168258No Hit
CAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAG114890.19360602984454897No Hit
GGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTC111040.18711823095081137No Hit
GATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGG107990.18197854611291533No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTATG107950.18191114041012324TruSeq Adapter, Index 27 (97% over 44bp)
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAG106790.179956375029153No Hit
CCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACC98410.1658348802942124No Hit
GTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATC93930.15828544158149957No Hit
TTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT93190.1570384360798461No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTG90280.15213467120172233No Hit
CGGCAATCGGGCGGCGGGCGCATGCGTCGCTTCTAGCCCGGATTCTGACTT79770.13442382279310358No Hit
TCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAAC79520.1340025371506531No Hit
CTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGA72540.12224024201343531No Hit
CCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAG71830.1210437907888759No Hit
TATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAG66390.11187661520915317No Hit
ATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGT62290.10496753067296505No Hit
TGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCTAGGTTA59820.10080522852555418No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG16150.032.87896711
ACGTCTG16600.031.58184415
TCGTATG15850.031.23079945
CACACGT17600.030.16993512
TTTTTCC82650.030.1449951
CTCGTAT16550.030.04581344
AGCACAC17900.029.91593710
GTACTCT36300.029.5661053
TCTAGTT37700.029.5414337
TACTCTA37050.029.2712444
CTCTAGT39500.028.5944426
TGTACTC37950.028.3994372
ACACGTC18750.028.07951513
TTGTACT38500.027.9432951
TAGTTGT38750.027.7541289
CACGTCT19300.027.1636614
ACTCTAG41500.027.1628685
GTCACAT19350.026.74372529
TATCTCG19000.026.52675241
TCGGGCG16800.026.3830287
Produced by FastQC (version 0.11.2)