FastQCFastQC Report
Tue 28 Mar 2017
HGJJWBCXY_l01n01_transplanta1_25.3510000008378e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGJJWBCXY_l01n01_transplanta1_25.3510000008378e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6437738
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAG257510.4000007456035024No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTA160780.24974610647404416No Hit
TTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAA112890.17535662370851376No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAGCATCTCGTATGCC106260.16505797533232947TruSeq Adapter, Index 1 (97% over 37bp)
CCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACC88970.13820071584149587No Hit
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAG73280.11382880135848959No Hit
CGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGT71440.11097065459948821No Hit
CAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAG69250.1075688386200246No Hit
TTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT66360.10307968419963658No Hit
CCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTA65640.1019612789461143No Hit
TCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAAC64810.10067200622330391No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG15400.033.75016811
CGTATGC14350.033.24032644
GTATGCC14750.032.6439845
ACGTCTG15500.032.51704815
AGCACAC16950.031.32734910
TCGTATG15250.030.9835243
CTCGTAT16150.029.67483942
CACGTCT18250.027.7405114
GAGCACA19500.026.9995069
GTCACAT18100.026.6019329
CACACGT19500.026.53859512
CATCTCG18650.025.69737839
CATGAGC19550.025.08924933
TTTTTCC53000.024.8838621
ACACGTC20900.024.86854413
TCTAGTT24350.024.6706227
CACATGA20000.024.6372431
ACATGAG20300.024.16249532
CAGTCAC20450.023.76484727
GTACTCT24050.022.9207553
Produced by FastQC (version 0.11.2)