FastQCFastQC Report
Tue 28 Mar 2017
HGJJWBCXY_l01n01_transplanta1_24.35100000083758.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGJJWBCXY_l01n01_transplanta1_24.35100000083758.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6150834
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAG282840.4598400802232673No Hit
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAG169310.2752634845941217No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTATG151720.24666573671147685TruSeq Adapter, Index 25 (97% over 44bp)
CGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGT138390.22499387887886424No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTA108320.1761061995820404No Hit
GATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGG98360.15991327354957066No Hit
CCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTA97860.15910037565637442No Hit
CAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAG86590.14077765714373042No Hit
GGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTC83130.13515240372281223No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAG79910.12991734129062824No Hit
GTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATC75820.12326783652428272No Hit
CTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGA71200.11575665999114916No Hit
TTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAA68160.11081424080051584No Hit
CGGCAATCGGGCGGCGGGCGCATGCGTCGCTTCTAGCCCGGATTCTGACTT67830.11027772819100629No Hit
ATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGT64140.1042785417392178No Hit
CCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAG63860.10382331891902788No Hit
CCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACC63670.1035144177196133No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG21250.035.4719611
TCGTATG20550.033.50303645
ACGTCTG22750.033.1326115
CACACGT23150.032.6573212
AGCACAC23800.032.0495610
CACGTCT23700.031.89944814
ACACGTC23850.031.6044813
CTCGTAT22950.030.68571544
TATCTCG23800.029.4952641
CACACTG26100.027.50016831
ACTGATA26350.027.23881334
GAGCACA28700.026.8122319
CAGTCAC26950.026.79957627
TATATCT26650.026.42562539
TCGGAAG28850.026.2826583
TACTCTA29900.026.187454
ACTCCAG29050.025.9457523
TGATATA27250.025.76099636
GTACTCT30150.025.6717993
TCCAGTC29900.025.58460825
Produced by FastQC (version 0.11.2)