FastQCFastQC Report
Tue 28 Mar 2017
HGJJWBCXY_l01n01_transplanta1_23.3510000008373e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGJJWBCXY_l01n01_transplanta1_23.3510000008373e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17388790
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAG353190.20311361515091045No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTATGCC302750.1741064214358791TruSeq Adapter, Index 22 (97% over 37bp)
CTGACATGTGTGCGAGTCAACGGGTGAGTAAACCCGTAAGGCGCAAGGAAG221240.12723139447885679No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGTAAGTAT213500.12278025095478179No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTA204640.117685014310944No Hit
CCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAGCGGAGTCCTATAA184690.10621210561516932No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC41400.035.162844
GCACACG43500.034.3967111
ACGTCTG45600.032.8121815
GTATGCC44750.032.42994345
TCGTATG45550.031.95916643
AGTCACG46250.031.42699228
CACACGT48800.030.89082312
GTCACGG48300.030.13971529
CTCGTAT49100.029.74012242
GGTAGCA49350.029.49937634
CACGTCT51200.029.13545814
AGCACAC55000.027.61376210
GTAGCAT53100.027.45846635
CGGTAGC54250.026.7504333
CATCTCG56150.026.1672439
ACGGTAG58800.024.75762432
TCCAGTC62900.024.00173625
TCGGAAG62100.023.9849973
ACACGTC62500.023.9754913
GAGCACA65600.023.6659959